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The map label for this gene is ispG [H]

Identifier: 49187177

GI number: 49187177

Start: 4096751

End: 4097863

Strand: Direct

Name: ispG [H]

Synonym: BAS4180

Alternate gene names: 49187177

Gene position: 4096751-4097863 (Clockwise)

Preceding gene: 49187176

Following gene: 49187182

Centisome position: 78.35

GC content: 38.63

Gene sequence:

>1113_bases
GTGAATGAAATGACTCATCGTACAAAAACACGTCCAGTTAAAGTCGGTAATTTAACAATTGGCGGTAATAATGAATTAAT
TATACAAAGTATGACAACAACAAAAACACATGATGTTGAAGCAACAGTTGCTGAAATTAAACGTTTAGAAGAAGCTGGCT
GTCAAGTCGTGCGTGTTGCTGTTCCAGACGAACGCGCAGCAAATGCCATTGCTGATATTAAAAAACAAATCAACATTCCA
CTTGTTGCTGATATTCATTTTGATTATCGCCTTGCTTTAAAAGCAATTGAAGGTGGCATTGATAAAGTACGTATCAATCC
AGGTAACATTGGTCGTCGCCATAAAGTAGAAGCTGTTGTAAATGCAGCAAAAGAACGCGGTATTCCAATCCGTATCGGTG
TAAACGCAGGTTCATTAGAACGTCACATTTTAGAAAAGTACGGATACCCAACTGCAGATGGTATGGTGGAGAGCGCCTTG
CATCATATTAAAATTTTAGAGGACTTAGATTTCCACGATATTATCGTATCTATGAAAGCCTCTGATGTTAACTTAGCAAT
TGAAGCATACGAAAAAGCTGCACGTGCTTTTGATTACCCATTACATTTAGGTATCACAGAATCTGGAACATTATTTGCCG
GAACTGTAAAAAGTGCTGCTGGTCTTGGAGCAATCTTAAATAAAGGTATCGGAAATACACTACGTATTTCACTTAGTGCT
GACCCAGTTGAAGAAGTAAAAGTTGCGCGTGAACTATTAAAGTCATTCGGCCTTGCATCTAATGCAGCAACACTTATTTC
TTGTCCAACTTGTGGTCGTATTGAAATTGATTTAATTAGCATCGCTAACGAGGTGGAAGAGTACATCTCTACACTGCAAG
TACCAATTAAAGTTGCCGTACTTGGTTGTGCTGTAAATGGTCCTGGTGAAGCTCGTGAAGCTGATATCGGTATTGCCGGT
GCACGCGGAGAAGGATTATTATTCCGTAAAGGGCAAGTTGTTCGTAAAGTACCAGAAGAAATAATGGTAGAAGAACTAAA
AAAAGAAATTGACGTAATTGCTGCTGAAATGGCTGCTGAACGAGAAAAAGAAAAAGAAACACAAGAACAATAA

Upstream 100 bases:

>100_bases
AAACCGAGCACTTCGGTTTCTATAGCCCTGTATCACGTATACTATCATTGATATAAGGGACTAAATAAGAAACTTCTTTT
ATAAGGAGAGCGATCTATTC

Downstream 100 bases:

>100_bases
AAAGAAGGTTGCCCACAATATTTGTGAGCAACCTTCTTTTATACACAAAAAAGCTCGTCAGTTCTATAACTGACGAGCTT
TTACTTTGCACATTTTGGAC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAANAIADIKKQINIP
LVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVVNAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAEREKEKETQEQ

Sequences:

>Translated_370_residues
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAANAIADIKKQINIP
LVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVVNAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAEREKEKETQEQ
>Mature_370_residues
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAANAIADIKKQINIP
LVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVVNAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAEREKEKETQEQ

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=365, Percent_Identity=47.3972602739726, Blast_Score=347, Evalue=7e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40028; Mature: 40028

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVA
CCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
VPDERAANAIADIKKQINIPLVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVV
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
NAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKA
HHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
CCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECC
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAV
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEE
LGCAVNGPGEAREADIGIAGARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAE
EEEEECCCCCCCCCCCEEECCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
REKEKETQEQ
HHHHHHHCCC
>Mature Secondary Structure
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVA
CCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
VPDERAANAIADIKKQINIPLVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVV
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
NAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKA
HHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
CCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECC
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAV
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEE
LGCAVNGPGEAREADIGIAGARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAE
EEEEECCCCCCCCCCCEEECCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
REKEKETQEQ
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA