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The map label for this gene is ldh [H]

Identifier: 49187068

GI number: 49187068

Start: 4001812

End: 4002912

Strand: Reverse

Name: ldh [H]

Synonym: BAS4070

Alternate gene names: 49187068

Gene position: 4002912-4001812 (Counterclockwise)

Preceding gene: 49187069

Following gene: 49187067

Centisome position: 76.56

GC content: 39.33

Gene sequence:

>1101_bases
ATGGCATTAGAAATCTTCGAATACTTAGAAAAATATGATTATGAGCAAGTAGTATTTTGTCAAGATAAAGAATCAGGTTT
AAAAGCAATCATTGCAATTCATGATACAACACTTGGACCTGCTCTTGGTGGAACAAGAATGTGGACATATGATTCTGAAG
AAGCGGCGATTGAAGATGCATTGCGTCTTGCAAAAGGGATGACATACAAAAACGCAGCAGCTGGTTTAAACTTAGGTGGT
GCGAAAACAGTAATTATCGGTGATCCACGTAAAGATAAGAGCGAAGCGATGTTCCGTGCATTAGGTCGTTATATTCAAGG
ACTAAACGGACGTTACATTACAGCTGAAGATGTTGGTACAACAGTAGATGATATGGATATTATCCACGAAGAAACTGACT
TTGTAACAGGTATTTCACCATCATTCGGTTCTTCTGGTAACCCATCTCCAGTAACTGCATACGGTGTTTATCGTGGTATG
AAAGCTGCTGCGAAAGAAGCTTTCGGTACTGATAACTTAGAAGGAAAAGTAATTGCTGTTCAAGGCGTTGGTAACGTAGC
ATACCACCTATGCAAACATTTACACGCCGAAGGAGCAAAACTAATTGTTACTGATATTAATAAAGAAGCTGTACAACGTG
CAGTAGAAGAATTCGGTGCAACAGCAGTTGAGCCAAATGAAATTTACGGTGTTGAATGTGATATTTACGCACCATGTGCA
TTAGGCGCAACAGTTAATGACGAAACTATTCCACAACTTAAAGCAAAAGTAATTGCAGGTTCTGCAAATAACCAATTAAA
AGAAGATCGTCACGGTGACATCATTCATGAAATGGGTATTGTATACGCACCAGACTATGTAATTAATGCAGGTGGCGTAA
TTAACGTAGCAGACGAGTTATATGGATACAATAGAGAACGTGCATTAAAACGCGTAGAATCAATTTATGACACAATTGCA
AAGGTAATCGAAATTTCAAAACGCGATGGCATTGCAACTTATGTAGCAGCTGATCGTCTAGCTGAAGAGCGCATTGCAAG
CTTGAAAAACTCTCGTAGCACATACTTACGCAACGGTCACGACATTATTAGCCGTCGCTAA

Upstream 100 bases:

>100_bases
CGTGCTGATTCAGCAGAAACAAAAGTATATTCATTAGCATTGGCAGTTGCCACTGCTTCAAAATAAACCAAATAGGGTAA
AACATTAGGGGGAAACGACA

Downstream 100 bases:

>100_bases
TCATTCATATATTACTTTTACAAAAGGTGTGGTTACCTCTTATGAGGTTTCCACCTCCTTTTGAATTTATTAGTGGAGGT
AGCAACAGTGTCTGTAAATC

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDALRLAKGMTYKNAAAGLNLGG
AKTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGTTVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGM
KAAAKEAFGTDNLEGKVIAVQGVGNVAYHLCKHLHAEGAKLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCA
LGATVNDETIPQLKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERALKRVESIYDTIA
KVIEISKRDGIATYVAADRLAEERIASLKNSRSTYLRNGHDIISRR

Sequences:

>Translated_366_residues
MALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDALRLAKGMTYKNAAAGLNLGG
AKTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGTTVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGM
KAAAKEAFGTDNLEGKVIAVQGVGNVAYHLCKHLHAEGAKLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCA
LGATVNDETIPQLKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERALKRVESIYDTIA
KVIEISKRDGIATYVAADRLAEERIASLKNSRSTYLRNGHDIISRR
>Mature_365_residues
ALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDALRLAKGMTYKNAAAGLNLGGA
KTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGTTVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGMK
AAAKEAFGTDNLEGKVIAVQGVGNVAYHLCKHLHAEGAKLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCAL
GATVNDETIPQLKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERALKRVESIYDTIAK
VIEISKRDGIATYVAADRLAEERIASLKNSRSTYLRNGHDIISRR

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=304, Percent_Identity=27.3026315789474, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI4885281, Length=304, Percent_Identity=26.6447368421053, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649283, Length=296, Percent_Identity=26.0135135135135, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 39851; Mature: 39720

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDA
CCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHH
LRLAKGMTYKNAAAGLNLGGAKTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGT
HHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHCCCC
TVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGMKAAAKEAFGTDNLEGKVIAV
CHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEE
QGVGNVAYHLCKHLHAEGAKLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCA
ECCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECEECCEE
LGATVNDETIPQLKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADEL
CCCCCCCCHHHHHHHHEEECCCCCCHHHCCCCCHHHHCCCEECCCEEEECCCEEEEHHHH
YGYNRERALKRVESIYDTIAKVIEISKRDGIATYVAADRLAEERIASLKNSRSTYLRNGH
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHCCCH
DIISRR
HHHCCC
>Mature Secondary Structure 
ALEIFEYLEKYDYEQVVFCQDKESGLKAIIAIHDTTLGPALGGTRMWTYDSEEAAIEDA
CHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHH
LRLAKGMTYKNAAAGLNLGGAKTVIIGDPRKDKSEAMFRALGRYIQGLNGRYITAEDVGT
HHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHCCCC
TVDDMDIIHEETDFVTGISPSFGSSGNPSPVTAYGVYRGMKAAAKEAFGTDNLEGKVIAV
CHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEE
QGVGNVAYHLCKHLHAEGAKLIVTDINKEAVQRAVEEFGATAVEPNEIYGVECDIYAPCA
ECCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECEECCEE
LGATVNDETIPQLKAKVIAGSANNQLKEDRHGDIIHEMGIVYAPDYVINAGGVINVADEL
CCCCCCCCHHHHHHHHEEECCCCCCHHHCCCCCHHHHCCCEECCCEEEECCCEEEEHHHH
YGYNRERALKRVESIYDTIAKVIEISKRDGIATYVAADRLAEERIASLKNSRSTYLRNGH
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHCCCH
DIISRR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9188201 [H]