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The map label for this gene is bioB [H]

Identifier: 49187021

GI number: 49187021

Start: 3958794

End: 3959792

Strand: Reverse

Name: bioB [H]

Synonym: BAS4023

Alternate gene names: 49187021

Gene position: 3959792-3958794 (Counterclockwise)

Preceding gene: 49187022

Following gene: 49187020

Centisome position: 75.73

GC content: 38.14

Gene sequence:

>999_bases
ATGAAACAAGTACAAACAAAAAGGGATTGGAAAAAACTTGCATATGACGTAGTAGAAGAAAAAATGATTACGAAAGAGGA
TGCAATAGCGATATTAGAAGCTGATGATACAGAAGTTTTAGAGATTATGAATGCTGCTTACATTATTCGCCATCATTACT
TTGGTAAGAAAGTAAAATTAAATATGATTATTAATACGAAATCTGGATTATGTCCTGAAGATTGCGGATATTGTTCACAG
TCGATTATTTCAGAAGCACCAATCGATAAATATGCATGGCTAACGCAGGAAAAGATTGTAGAAGGAGCACACGAAGCAAT
ACGCCGTAAAGCTGGTACATATTGTATTGTAGCATCTGGTCGTCGCCCGACGGATAAAGAGGTGAATCACGTAATTGGAG
CAGTTAAGGAGATTCGTGAAACGACAGATTTGAAGATTTGTTGTTGTTTAGGTTTCTTAAACGAAGATCAAGCAGGACGT
TTAGCTGAAGCTGGTGTACATCGTTATAACCACAACTTAAATACACATGCGAATAATTACGAAAGCATTTGCTCCACTCA
TACGTATGATGATCGTGTTGATACAGTTCAAAAAGCGAAACAAGCGGGTATTTCACCATGTTCTGGGGCAATCTTTGGAA
TGGGAGAGACAATAGAAGAGCGCGCTGAAATTGCATTCGAATTACAACGTATAGATGCAGATTCAATTCCATGTAATTTC
CTTGTTGCTGTAAAGGGTACGCCGCTTGAAGGACAAAAGGAGTTAACGCCAGTAGAATGTTTAAAAGTGTTGGCAATGAT
GCGTTTTGTAAACCCAACAAAAGAAATTCGTATTTCAGGAGGGCGTGAAATCAATTTACGTTCTGTACAGCCAATCGGTT
TATTTGCAGCAAACTCTATTTTTGTTGGAGACTACTTAACGACAGCTGGACAAGAACCGACTGCAGACTGGGGTATGATT
GAAGATTTAGGATTTGAGATTGAAGAATGTGCGTTATAA

Upstream 100 bases:

>100_bases
ATGCTTCGCATATATGAAAGAGACTTCACGGGAAATGAAGGGATAATGGCGACGTATCATGCTTTATTTATGCATATAAC
AAAAGAGGGGAAGAGATGAA

Downstream 100 bases:

>100_bases
GATAGAGGAAAAAGTAAACTTGTAAAAGAGTTTGCTTTTTTTGTAAATAAAATGTAATAAATAGTTGTGAACGCTTGATA
CTAGTGGCTTTTGAGCGAAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKQVQTKRDWKKLAYDVVEEKMITKEDAIAILEADDTEVLEIMNAAYIIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGR
LAEAGVHRYNHNLNTHANNYESICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSIFVGDYLTTAGQEPTADWGMI
EDLGFEIEECAL

Sequences:

>Translated_332_residues
MKQVQTKRDWKKLAYDVVEEKMITKEDAIAILEADDTEVLEIMNAAYIIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGR
LAEAGVHRYNHNLNTHANNYESICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSIFVGDYLTTAGQEPTADWGMI
EDLGFEIEECAL
>Mature_332_residues
MKQVQTKRDWKKLAYDVVEEKMITKEDAIAILEADDTEVLEIMNAAYIIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGR
LAEAGVHRYNHNLNTHANNYESICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSIFVGDYLTTAGQEPTADWGMI
EDLGFEIEECAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=295, Percent_Identity=34.2372881355932, Blast_Score=189, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321725, Length=309, Percent_Identity=32.0388349514563, Blast_Score=159, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36997; Mature: 36997

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVQTKRDWKKLAYDVVEEKMITKEDAIAILEADDTEVLEIMNAAYIIRHHYFGKKVKL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEE
NMIINTKSGLCPEDCGYCSQSIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASG
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
RRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGRLAEAGVHRYNHNLNTHANNY
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCH
ESICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSI
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCEEEEECCE
FVGDYLTTAGQEPTADWGMIEDLGFEIEECAL
EECCHHHHCCCCCCCCCCCHHHHCCCHHHHCC
>Mature Secondary Structure
MKQVQTKRDWKKLAYDVVEEKMITKEDAIAILEADDTEVLEIMNAAYIIRHHYFGKKVKL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEE
NMIINTKSGLCPEDCGYCSQSIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASG
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
RRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGRLAEAGVHRYNHNLNTHANNY
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCH
ESICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSI
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCEEEEECCE
FVGDYLTTAGQEPTADWGMIEDLGFEIEECAL
EECCHHHHCCCCCCCCCCCHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA