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The map label for this gene is yhcR [H]

Identifier: 49187007

GI number: 49187007

Start: 3946359

End: 3947948

Strand: Direct

Name: yhcR [H]

Synonym: BAS4009

Alternate gene names: 49187007

Gene position: 3946359-3947948 (Clockwise)

Preceding gene: 49187003

Following gene: 49187009

Centisome position: 75.48

GC content: 37.11

Gene sequence:

>1590_bases
ATGTGGAAAAAAATCATTCCTGCTGTTGCCGTTTTAAGTACCATTACTTTTTCAAGCGTATTTGCCGCTCCCCCATCTCA
GACTCCAGCTGAACAAAACCGCTACATAGACGTACAAATACTTGGTATTAATGATTTCCATGGACAACTAGATACTGTTA
AAAAAATTAATAACAAAGAAGCTGGTGGCGCTGATTACTTAGCTACTTATTTAAAAGAACGTAAAAAACAAAACCCTAAT
ACACTTCTTGTACATGCTGGTGATATTGTAGGTGCTAGTCCACCAGTTTCAGCATTATTACAAGATGAGCCTACAATTGA
ATTCTTAAATGATTTAAAATTTGATGTTGGTACAATCGGAAACCACGAATTTGATGAAGGTATTGAGGAAATGCGCCGCC
TCATTTATGGCGGTTACCATGAGAAAACTGGCAATTTTAAAGGCGCAGACTTCCCTTATGTCGCTGCAAACTTCTATAAT
AAATCCACTGGCCGCTTATTTTTACCGCCATTTACTATCAAAATGGTAAATGGTGTGCCTGTAGGATTCATTGGCGTTGT
TACAACTGATACACCAAATGTCGTGATGCCTACTATGCTTAAAAATGTAGAAATCACTGACGAAGTAGAAGCTATCAATA
AATCAGCACAACAATTAAAACGTCTTGGCGTTAAATCCATGGTCGTCCTTGCTCATGTTGGCGGAACAACAGATGAATCT
GGTGTGACAAATGGAGACCTTACTCGAATTGCAAATGAAACAGATCCGGAAGTTGATGTTATTTTTGGTGGGCATAGTCA
TACGTATGTAAATGGTACTGTAAATAATAAACTAATCGTCCAAGCGAACTCTTACGGAACAGCTTTCTCAGACGTAGATG
TAACAATTGATCGTAAAACAAAAGATATTGTAAAGAAAAAAGCTGAAGTTGTTACAACATACCATGAAGGTGTAGAACCT
GATAAACAAGTAAAGAAAAAATTAGATCAGTATAAAGAAAAAATCGCACCACTTGTAAATGAAGTTGTCGGAAAATCTAC
AGCGCCTATCGACCGTAAACAAAATGATGCTGGTGAGTCCACTCTTGGAAATTTAGTTGCCGATGCCCAGCGCCAAACGA
TGCAAACTCAAATCGCACTTATGAATCCAGGTGGCATTCGTAATGACTTAGATGCTGGTGATATTACATGGGGCGAATTA
TACGGCATTCAGCCTTTTGGAAACCAATTAATTAAAGTAGATTTAACAGGCCAAGATATTCGTGACATTTTAAATCAACA
ATGGCAAAAAGGAACGACGAGAATGCTTCAAATTTCAGGTATTCAATATACGTGGGATGCAAATAAACCAAACGGTGAAA
AAGTTACAAATATACGTTTAACAAGCGGAGAAGAATTATCTCCATCTAAAACGTATAGCGTAGTTGCGAATGCTTTCTTA
GCTTCTGGCGGTGATGGATTTGTATCATTTAAAAATGGTAAAAATACAGAAACTGGTCCAAACGACTTTGAAGCGCTAGT
CGATTACGTAAAACAATTAAAAGAACCTATTCAGCCAATCATCGATGGTAGAATTCAAAAAATAAATTAA

Upstream 100 bases:

>100_bases
AATTTTTCGAAAAAACTAAATTTTTATATAGATATAATCCTATGAATCTATTAAAATTATGTCGTATGATGTCAGATTAT
AAAAAAAGGAGAATTATATT

Downstream 100 bases:

>100_bases
GTACACTCATAATAAACAGTCTTAATTTTTTATCGTAAAAAATACACATGGACCTTTTATTTTATCCGTCATATATGTGA
AACCTAAAAAGGATACTTAC

Product: 5'-nucleotidase family protein

Products: ribonucleoside; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MWKKIIPAVAVLSTITFSSVFAAPPSQTPAEQNRYIDVQILGINDFHGQLDTVKKINNKEAGGADYLATYLKERKKQNPN
TLLVHAGDIVGASPPVSALLQDEPTIEFLNDLKFDVGTIGNHEFDEGIEEMRRLIYGGYHEKTGNFKGADFPYVAANFYN
KSTGRLFLPPFTIKMVNGVPVGFIGVVTTDTPNVVMPTMLKNVEITDEVEAINKSAQQLKRLGVKSMVVLAHVGGTTDES
GVTNGDLTRIANETDPEVDVIFGGHSHTYVNGTVNNKLIVQANSYGTAFSDVDVTIDRKTKDIVKKKAEVVTTYHEGVEP
DKQVKKKLDQYKEKIAPLVNEVVGKSTAPIDRKQNDAGESTLGNLVADAQRQTMQTQIALMNPGGIRNDLDAGDITWGEL
YGIQPFGNQLIKVDLTGQDIRDILNQQWQKGTTRMLQISGIQYTWDANKPNGEKVTNIRLTSGEELSPSKTYSVVANAFL
ASGGDGFVSFKNGKNTETGPNDFEALVDYVKQLKEPIQPIIDGRIQKIN

Sequences:

>Translated_529_residues
MWKKIIPAVAVLSTITFSSVFAAPPSQTPAEQNRYIDVQILGINDFHGQLDTVKKINNKEAGGADYLATYLKERKKQNPN
TLLVHAGDIVGASPPVSALLQDEPTIEFLNDLKFDVGTIGNHEFDEGIEEMRRLIYGGYHEKTGNFKGADFPYVAANFYN
KSTGRLFLPPFTIKMVNGVPVGFIGVVTTDTPNVVMPTMLKNVEITDEVEAINKSAQQLKRLGVKSMVVLAHVGGTTDES
GVTNGDLTRIANETDPEVDVIFGGHSHTYVNGTVNNKLIVQANSYGTAFSDVDVTIDRKTKDIVKKKAEVVTTYHEGVEP
DKQVKKKLDQYKEKIAPLVNEVVGKSTAPIDRKQNDAGESTLGNLVADAQRQTMQTQIALMNPGGIRNDLDAGDITWGEL
YGIQPFGNQLIKVDLTGQDIRDILNQQWQKGTTRMLQISGIQYTWDANKPNGEKVTNIRLTSGEELSPSKTYSVVANAFL
ASGGDGFVSFKNGKNTETGPNDFEALVDYVKQLKEPIQPIIDGRIQKIN
>Mature_529_residues
MWKKIIPAVAVLSTITFSSVFAAPPSQTPAEQNRYIDVQILGINDFHGQLDTVKKINNKEAGGADYLATYLKERKKQNPN
TLLVHAGDIVGASPPVSALLQDEPTIEFLNDLKFDVGTIGNHEFDEGIEEMRRLIYGGYHEKTGNFKGADFPYVAANFYN
KSTGRLFLPPFTIKMVNGVPVGFIGVVTTDTPNVVMPTMLKNVEITDEVEAINKSAQQLKRLGVKSMVVLAHVGGTTDES
GVTNGDLTRIANETDPEVDVIFGGHSHTYVNGTVNNKLIVQANSYGTAFSDVDVTIDRKTKDIVKKKAEVVTTYHEGVEP
DKQVKKKLDQYKEKIAPLVNEVVGKSTAPIDRKQNDAGESTLGNLVADAQRQTMQTQIALMNPGGIRNDLDAGDITWGEL
YGIQPFGNQLIKVDLTGQDIRDILNQQWQKGTTRMLQISGIQYTWDANKPNGEKVTNIRLTSGEELSPSKTYSVVANAFL
ASGGDGFVSFKNGKNTETGPNDFEALVDYVKQLKEPIQPIIDGRIQKIN

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=537, Percent_Identity=25.3258845437616, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=25.1366120218579, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1790658, Length=569, Percent_Identity=22.1441124780316, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922446, Length=557, Percent_Identity=28.1867145421903, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24654424, Length=557, Percent_Identity=28.1867145421903, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24641187, Length=550, Percent_Identity=25.8181818181818, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI221329836, Length=551, Percent_Identity=25.7713248638838, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19921980, Length=449, Percent_Identity=25.6124721603563, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24652512, Length=449, Percent_Identity=25.6124721603563, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI161076508, Length=449, Percent_Identity=25.6124721603563, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922444, Length=575, Percent_Identity=25.5652173913043, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28573524, Length=464, Percent_Identity=25.4310344827586, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.3.5

Molecular weight: Translated: 57988; Mature: 57988

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKKIIPAVAVLSTITFSSVFAAPPSQTPAEQNRYIDVQILGINDFHGQLDTVKKINNKE
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCC
AGGADYLATYLKERKKQNPNTLLVHAGDIVGASPPVSALLQDEPTIEFLNDLKFDVGTIG
CCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCC
NHEFDEGIEEMRRLIYGGYHEKTGNFKGADFPYVAANFYNKSTGRLFLPPFTIKMVNGVP
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCEEEEEECCCC
VGFIGVVTTDTPNVVMPTMLKNVEITDEVEAINKSAQQLKRLGVKSMVVLAHVGGTTDES
EEEEEEEECCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCC
GVTNGDLTRIANETDPEVDVIFGGHSHTYVNGTVNNKLIVQANSYGTAFSDVDVTIDRKT
CCCCCCHHHHCCCCCCCEEEEECCCCEEEEECCCCCEEEEEECCCCCEECCEEEEECCCH
KDIVKKKAEVVTTYHEGVEPDKQVKKKLDQYKEKIAPLVNEVVGKSTAPIDRKQNDAGES
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
TLGNLVADAQRQTMQTQIALMNPGGIRNDLDAGDITWGELYGIQPFGNQLIKVDLTGQDI
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHEECCCCCCCEEEEEEECCHHH
RDILNQQWQKGTTRMLQISGIQYTWDANKPNGEKVTNIRLTSGEELSPSKTYSVVANAFL
HHHHHHHHHCCCCEEEEEECEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
ASGGDGFVSFKNGKNTETGPNDFEALVDYVKQLKEPIQPIIDGRIQKIN
HCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure
MWKKIIPAVAVLSTITFSSVFAAPPSQTPAEQNRYIDVQILGINDFHGQLDTVKKINNKE
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCC
AGGADYLATYLKERKKQNPNTLLVHAGDIVGASPPVSALLQDEPTIEFLNDLKFDVGTIG
CCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCC
NHEFDEGIEEMRRLIYGGYHEKTGNFKGADFPYVAANFYNKSTGRLFLPPFTIKMVNGVP
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCEEEEEECCCC
VGFIGVVTTDTPNVVMPTMLKNVEITDEVEAINKSAQQLKRLGVKSMVVLAHVGGTTDES
EEEEEEEECCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCC
GVTNGDLTRIANETDPEVDVIFGGHSHTYVNGTVNNKLIVQANSYGTAFSDVDVTIDRKT
CCCCCCHHHHCCCCCCCEEEEECCCCEEEEECCCCCEEEEEECCCCCEECCEEEEECCCH
KDIVKKKAEVVTTYHEGVEPDKQVKKKLDQYKEKIAPLVNEVVGKSTAPIDRKQNDAGES
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
TLGNLVADAQRQTMQTQIALMNPGGIRNDLDAGDITWGELYGIQPFGNQLIKVDLTGQDI
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHEECCCCCCCEEEEEEECCHHH
RDILNQQWQKGTTRMLQISGIQYTWDANKPNGEKVTNIRLTSGEELSPSKTYSVVANAFL
HHHHHHHHHCCCCEEEEEECEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
ASGGDGFVSFKNGKNTETGPNDFEALVDYVKQLKEPIQPIIDGRIQKIN
HCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5'-ribonucleotide; H2O

Specific reaction: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]