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The map label for this gene is dacF [H]

Identifier: 49186984

GI number: 49186984

Start: 3926657

End: 3927847

Strand: Reverse

Name: dacF [H]

Synonym: BAS3986

Alternate gene names: 49186984

Gene position: 3927847-3926657 (Counterclockwise)

Preceding gene: 49186988

Following gene: 49186983

Centisome position: 75.12

GC content: 36.27

Gene sequence:

>1191_bases
ATGAAGCGAGTTTTTGGAATACTTGTTTGTTTCATGTTATTGCTTTCGGGTACTTCAGTTAGTTTCGCACAATCTGAGAA
AACGAAGCAGGATAAAACAGAAGAAACAACGCCGAAGTTAGCAGAACAAGCGTCGTCAGCAATTGTAATTGAACAAGATA
CAGGTAAAGTTTTGTTTGATAAAAATCCAAATGAGAAATTACCGCCTGCTAGTATGACGAAGATTATGACAATGCTATTA
ATTATGGAACAAGTTGAAAAAGGAAAATTAAAACTGACCGATAAAGTTAGAGCGAGTGAGCATGCAGCTTCAATGGGTGG
ATCACAAATCTTTTTAGAGCCTGGAGAAGAGATGACTGTAAATGAAATGCTAAAGGGTATTGCAATTGCATCTGGAAATG
ATGCGTCTGTTGCAGTAGCGGAGCATATCGCTGGTTCAGAAGAAGGTTTTGTAAATATGATGAACAAAAAAGCGAAAGAT
TTAGGGCTAAAAAATACTCATTTTCAAAATCCAACAGGTCTTCCGGCTAAAGACCATTATTCTACAGCAAATGATATGGC
TATCATGGCGAAAGAATTGATGAAGTACCCACTTATTCGCAAATACACAGGTAAATACGAAGACTATTTACGTGAAGATA
CGGATAAGAAGTTTTGGCTCGTTAATACGAATAAGTTAGTACGTTTTTATCCTGGAGTAGATGGTGTAAAAACGGGCTTT
ACGACAGAAGCAAAATATTGTTTAACAGCATCGGCTGAGAAGAATGGGATGCGTGTTATTTCAGTTGTTATGGGAGCACC
TACATCAAAAGAACGGAACAATCAAGTAACGAAGCTTCTTGACTACGCATTTGGTCAATATATGACAAAAAAATTGTATA
CACGAGGCGAAAAAATTAAAACTGTCCAAGTAGGAAAAGGGAAAAAAGAAAAAGTAGATTTAGTTGCGTCAGACAATGTA
TCTCTTCTTATGAAGAAGGGCGAAAATATGGACAAGGTAAAGCAAGAAGTAATTGCTGAAAAGAAAGTGAAAGCGCCGAT
TAAAAAAGGTGATGCACTTGGTACGCTTGTTATTAAAAAAGATAAAGATGTTTTATTAAAACAAACAATTGTAGCAAAAG
AAGATGTTGCTGCAGCGAGCTGGTGGGAGTTATTTAAAAGAAGTTTTGGGATGTTTTCAACATCAAAATAG

Upstream 100 bases:

>100_bases
GGTGATAAATGTGTTTGTCAACTGAAAATGTTAGAAAAATATGGTATTAAAAAATAATAAATTGGAAAAAATAGGTTTGA
TATAGAATGGAGGGTTGGTC

Downstream 100 bases:

>100_bases
CGGCAAATATTTTGCCGCTTTTCTATTCGTATGTAAGTAAATAATTGCTGAATGGTTTCACTTTATGGCGAAATAGTTCT
TCTTTTGTCAGTAGGAAGGA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVLFDKNPNEKLPPASMTKIMTMLL
IMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKAKD
LGLKNTHFQNPTGLPAKDHYSTANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKTGF
TTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEKIKTVQVGKGKKEKVDLVASDNV
SLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVIKKDKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK

Sequences:

>Translated_396_residues
MKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVLFDKNPNEKLPPASMTKIMTMLL
IMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKAKD
LGLKNTHFQNPTGLPAKDHYSTANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKTGF
TTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEKIKTVQVGKGKKEKVDLVASDNV
SLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVIKKDKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK
>Mature_396_residues
MKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVLFDKNPNEKLPPASMTKIMTMLL
IMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKAKD
LGLKNTHFQNPTGLPAKDHYSTANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKTGF
TTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEKIKTVQVGKGKKEKVDLVASDNV
SLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVIKKDKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=354, Percent_Identity=35.0282485875706, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1786851, Length=347, Percent_Identity=34.5821325648415, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082022, Length=364, Percent_Identity=29.6703296703297, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082054, Length=253, Percent_Identity=31.2252964426877, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43961; Mature: 43961

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVLFD
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCEEEC
KNPNEKLPPASMTKIMTMLLIMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEMTV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCH
NEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKAKDLGLKNTHFQNPTGLPAKDHY
HHHHHHEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
STANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKTGF
CCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCC
TTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEKIK
CCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
TVQVGKGKKEKVDLVASDNVSLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVIKK
EEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
DKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVLFD
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCEEEC
KNPNEKLPPASMTKIMTMLLIMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEMTV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCH
NEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKAKDLGLKNTHFQNPTGLPAKDHY
HHHHHHEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
STANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKTGF
CCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCC
TTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEKIK
CCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
TVQVGKGKKEKVDLVASDNVSLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVIKK
EEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
DKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]