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The map label for this gene is nplT [H]

Identifier: 49186921

GI number: 49186921

Start: 3872447

End: 3874207

Strand: Direct

Name: nplT [H]

Synonym: BAS3923

Alternate gene names: 49186921

Gene position: 3872447-3874207 (Clockwise)

Preceding gene: 49186916

Following gene: 49186923

Centisome position: 74.06

GC content: 35.66

Gene sequence:

>1761_bases
ATGCTTAAAGAAGCCATTTATCATAGGCCAAAAGATAATTACGCATACGCTTACGATGAAAAAACGATTCACATCCGAAT
ACGTACGAAAAGAGACGATGTTCAAAGCACCACTCTTATTTATGGTGATCCTTATGAATGGAAAGACGGGAAGTGGATTT
CTTCAAGTACACCGATGAAAAAAACTGGTTCTACTGCATTGTTTGATTATTGGTTCATTTCCATCGAGCCGAAATTTAAG
CGCTTACGTTATGGATTTGAATTAAAAAACGATACAGACACTCTTATTTATGCAGAGCGAGGATTTTTTTCTACCACTCC
AAATGATGACGTTGGTAACTTTTTCTGTTTTCCATTTATTCATGCAAATGATGTATTCAAAGCACCTTCTTGGATTAAAG
ATACTGTTTGGTATCAAATTTTCCCAGAACGGTTCGCTAATGGAGATCATACGCTGAATCCAGAAAACACCCTTCCTTGG
GGCAGCGCTGAGCCAACTCCAACTAATTTTTTCGGTGGAGATTTTGCTGGTATTATTCAAAACCTTGATTACCTTGTTAA
GCTTGGAATTTCAGGAATATATTTCACACCTATTTTCAAAGCACATTCAAACCATAAATATGACACAATTGACTACATGG
AAATCGATCCACAATTTGGTACAAAAGAAACATTCAAAGAACTCGTTCAGGCATGTCATACACACGGTATAAAAGTAATG
CTCGATGCTGTATTCAATCACAGTGGATACTTTTTCGATAAATTTCAAGACGTACTACAAAATGGTGAGCAGTCAGCATA
TAAAGAATGGTTCCATATTCATGAGTTCCCAATTAGAACTGAGCCACTCCCGAATTATGATACTTTCGCGTTTACACCGT
ATATGCCGAAATTAAACACAGCGCACCCGGATGTAAAAGAATACTTACTTGAAGTAGGGCGTTATTGGGTGAGAGAATTC
AATATAGACGGTTGGCGCCTTGATGTAGCAAATGAAGTCGACCATAACTTTTGGAGAGAATTTCGAAGTGAGATAAAAGC
ATTAAATTCTGAAGTATATATTTTAGGAGAAATTTGGCACGATGCACTTCCATGGCTACAAGGAGATCAATTCGATGCTG
TCATGAGCTATCCTGTTACAAACGCCCTACTGTCTTACTTTGCTAACGATTCCATTAAGGCAAATGAATTTATGAAACAA
ATTACAGAATCTCTACATTCCTACTCTATGAATGTAAATGAAGCAGCATTTCATTTATTAGATAGCCATGATACACCAAG
AATTTTGACAACATGTAACGGAGATAAAAATAAGTTAAAATTACTTTATGTATTCCATCTTTCTTTCATCGGCTCTCCTT
GTATTTATTATGGAGACGAAATTGGTATGGACGGCGGTATGGACCCAGATTGTCGCAAATGTATGGTTTGGGATACGGAA
GAACAAGATCATACATTATTTACACATGTACAAACATTAATTTCATTACGAAAGCAATATAAAGCATTTGGAGGACATGG
TACTTTCCAATTCATTGAAGCAAATGATGAATATAATTATATTTCTTATACGAAAACATATGAAGATGAAACGATCTTTT
TCGTTTTAAATCCGACTAATAGTGATATTACAGCTTCACTCCCTCTCCATGTTACTGGAAAGAAAATCATTAATATTTAT
ACAAACGAAGAATTTTCAGCGGAAGCAAGTGTATTACAAGTTACACTTCCTCCATATGGATTCTCCATATTAAAATGGTA
A

Upstream 100 bases:

>100_bases
AATATTAAAGCTATGCAAAAAGCAATCGTTTGCAACATATGCTTTATTATTATACGTAAGGGAACAATATGCATGAAGAT
AGTAAAGGGGGATTTTCTAT

Downstream 100 bases:

>100_bases
CCAAAAAAATCCTTATACCAAAGTGGTATAAGGATTTTTCTTATTTCAATTTGAATACCATTGCTTCATACGGACGTAAT
GGTATGTTTTCTATCGGTCC

Product: alpha-amylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMKKTGSTALFDYWFISIEPKFK
RLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFIHANDVFKAPSWIKDTVWYQIFPERFANGDHTLNPENTLPW
GSAEPTPTNFFGGDFAGIIQNLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKETFKELVQACHTHGIKVM
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNTAHPDVKEYLLEVGRYWVREF
NIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWHDALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQ
ITESLHSYSMNVNEAAFHLLDSHDTPRILTTCNGDKNKLKLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTNSDITASLPLHVTGKKIINIY
TNEEFSAEASVLQVTLPPYGFSILKW

Sequences:

>Translated_586_residues
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMKKTGSTALFDYWFISIEPKFK
RLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFIHANDVFKAPSWIKDTVWYQIFPERFANGDHTLNPENTLPW
GSAEPTPTNFFGGDFAGIIQNLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKETFKELVQACHTHGIKVM
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNTAHPDVKEYLLEVGRYWVREF
NIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWHDALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQ
ITESLHSYSMNVNEAAFHLLDSHDTPRILTTCNGDKNKLKLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTNSDITASLPLHVTGKKIINIY
TNEEFSAEASVLQVTLPPYGFSILKW
>Mature_586_residues
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMKKTGSTALFDYWFISIEPKFK
RLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFIHANDVFKAPSWIKDTVWYQIFPERFANGDHTLNPENTLPW
GSAEPTPTNFFGGDFAGIIQNLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKETFKELVQACHTHGIKVM
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNTAHPDVKEYLLEVGRYWVREF
NIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWHDALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQ
ITESLHSYSMNVNEAAFHLLDSHDTPRILTTCNGDKNKLKLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTNSDITASLPLHVTGKKIINIY
TNEEFSAEASVLQVTLPPYGFSILKW

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=409, Percent_Identity=23.4718826405868, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1786604, Length=507, Percent_Identity=31.9526627218935, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1790687, Length=394, Percent_Identity=25.6345177664975, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789995, Length=484, Percent_Identity=23.1404958677686, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32565753, Length=119, Percent_Identity=31.9327731092437, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=233, Percent_Identity=25.7510729613734, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319776, Length=405, Percent_Identity=21.4814814814815, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321731, Length=409, Percent_Identity=21.5158924205379, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321726, Length=229, Percent_Identity=24.4541484716157, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=229, Percent_Identity=24.4541484716157, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=229, Percent_Identity=24.4541484716157, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=229, Percent_Identity=24.4541484716157, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24586587, Length=424, Percent_Identity=23.8207547169811, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24586591, Length=408, Percent_Identity=23.0392156862745, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586593, Length=410, Percent_Identity=22.4390243902439, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586589, Length=401, Percent_Identity=24.4389027431421, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583747, Length=228, Percent_Identity=28.0701754385965, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583749, Length=228, Percent_Identity=28.0701754385965, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586599, Length=223, Percent_Identity=26.457399103139, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI221330053, Length=181, Percent_Identity=27.6243093922652, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45549022, Length=244, Percent_Identity=25.4098360655738, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583745, Length=223, Percent_Identity=27.3542600896861, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=25.3333333333333, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 68264; Mature: 68264

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMK
CCCHHHHCCCCCCEEEEECCEEEEEEEEECCCCCCCEEEEECCCCCCCCCEEECCCCCCC
KTGSTALFDYWFISIEPKFKRLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFI
CCCCEEEEEEEEEEECCHHHHHEECEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEEE
HANDVFKAPSWIKDTVWYQIFPERFANGDHTLNPENTLPWGSAEPTPTNFFGGDFAGIIQ
ECCCCCCCCCHHHHHHHEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
NLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKETFKELVQACHTHGIKVM
HHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHEE
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNT
EEHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCC
AHPDVKEYLLEVGRYWVREFNIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWH
CCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
DALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQITESLHSYSMNVNEAAFHLL
HHCCCCCCCCCCHHECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
DSHDTPRILTTCNGDKNKLKLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
CCCCCCEEEEEECCCCCCEEEEEEEEHHHHCCCCEEECCCCCCCCCCCCCCCEEEEECCC
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTN
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECCCC
SDITASLPLHVTGKKIINIYTNEEFSAEASVLQVTLPPYGFSILKW
CCEEEEEEEEECCCEEEEEEECCCCCCCCEEEEEEECCCCCEECCC
>Mature Secondary Structure
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMK
CCCHHHHCCCCCCEEEEECCEEEEEEEEECCCCCCCEEEEECCCCCCCCCEEECCCCCCC
KTGSTALFDYWFISIEPKFKRLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFI
CCCCEEEEEEEEEEECCHHHHHEECEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEEE
HANDVFKAPSWIKDTVWYQIFPERFANGDHTLNPENTLPWGSAEPTPTNFFGGDFAGIIQ
ECCCCCCCCCHHHHHHHEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
NLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKETFKELVQACHTHGIKVM
HHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHEE
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNT
EEHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCC
AHPDVKEYLLEVGRYWVREFNIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWH
CCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
DALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQITESLHSYSMNVNEAAFHLL
HHCCCCCCCCCCHHECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
DSHDTPRILTTCNGDKNKLKLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
CCCCCCEEEEEECCCCCCEEEEEEEEHHHHCCCCEEECCCCCCCCCCCCCCCEEEEECCC
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTN
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECCCC
SDITASLPLHVTGKKIINIYTNEEFSAEASVLQVTLPPYGFSILKW
CCEEEEEEEEECCCEEEEEEECCCCCCCCEEEEEEECCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]