Click here to switch to the map view.

The map label for this gene is dacA [H]

Identifier: 49185390

GI number: 49185390

Start: 2381451

End: 2382269

Strand: Reverse

Name: dacA [H]

Synonym: BAS2382

Alternate gene names: 49185390

Gene position: 2382269-2381451 (Counterclockwise)

Preceding gene: 49185391

Following gene: 49185376

Centisome position: 45.56

GC content: 32.72

Gene sequence:

>819_bases
ATGACAGAATATATCGTACTAGAACAACTGGATAAAGGAAATATACAGTTAGATGAAGTAGTAAAAATAAGTAACGAAGT
TTTCCGAGCAGAAACGAGTCCTATACAAGTAACTTCGAAAGATAAAACAACGGTTCGTGATTTACTTCATGCATTGTTAT
TGACAGGGAATAATCGTTCCACACTTGCCCTTGCAGAACACATTGCTGGAAACGAAGATAATTTCACTCAGCTAATGAAT
GAAAAAGCAAAACAACTAAAGTTATCACAACCATCTCCGTTTCTAAACGCTACTGGAATGAATAACGGTACAAATAAACT
TTCAACTACAACAGCAATTGAAGTTGGTAAACTAGCAACAAGATTAGTAACAGACTTTCCAGATGTACTGAACGTTACAA
AACTAACCTCTTACCAGTTCACCTTCAAAGATACCAAAGTTTTCAATACCAATAAAATGATTTATTCCCTAAATAAAAAC
ATTAAACTCCAAGGTGTTGATGGCTTACAAACTAGTTTTTCAACTAATAGTAATTATAGTTTTGTCAGTACAGCAAAACG
AGGAGATACTAGACTTATTTCAGTAATATTAGACGCCGATAATGAAAACAGTACTTTTATTGAAACAAAAAAATTACTAC
AGTACGGCTTTGATCCCTCCTCATACTCTGCACTCCAAGCATTTAAAGATGCTGTTACATCTTGGGCAGTTTTGCTTCAG
TTTAAAAATTTGATCATACAAACATTGTTGATTTTCTTTATTATTACAATTTTAATGCTTTTACATATACGTCAAAAAAA
ATCCGAGGATTTCAACTAG

Upstream 100 bases:

>100_bases
TTCAAGCTAAATCAGCTATTTTAATAGATGCCAATTCAGGTGAAGTTGTTTATAAAAAAATGAAGAAAATTCTATACAAT
CGGCTACTTTATCTAAATTA

Downstream 100 bases:

>100_bases
GAAATCTTCTTATATATAAAGAACAGCTTGCATTTACAGTGCAAGCCGTTTTCTAAATTTATAGTCCTCTCCCAATTAAA
TTCGCTGAAACAGGCCGAAG

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTEYIVLEQLDKGNIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLLTGNNRSTLALAEHIAGNEDNFTQLMN
EKAKQLKLSQPSPFLNATGMNNGTNKLSTTTAIEVGKLATRLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKN
IKLQGVDGLQTSFSTNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWAVLLQ
FKNLIIQTLLIFFIITILMLLHIRQKKSEDFN

Sequences:

>Translated_272_residues
MTEYIVLEQLDKGNIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLLTGNNRSTLALAEHIAGNEDNFTQLMN
EKAKQLKLSQPSPFLNATGMNNGTNKLSTTTAIEVGKLATRLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKN
IKLQGVDGLQTSFSTNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWAVLLQ
FKNLIIQTLLIFFIITILMLLHIRQKKSEDFN
>Mature_271_residues
TEYIVLEQLDKGNIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLLTGNNRSTLALAEHIAGNEDNFTQLMNE
KAKQLKLSQPSPFLNATGMNNGTNKLSTTTAIEVGKLATRLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNI
KLQGVDGLQTSFSTNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWAVLLQF
KNLIIQTLLIFFIITILMLLHIRQKKSEDFN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=223, Percent_Identity=30.4932735426009, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082022, Length=223, Percent_Identity=26.457399103139, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786851, Length=226, Percent_Identity=26.1061946902655, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 30565; Mature: 30434

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYIVLEQLDKGNIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLLTGNNRS
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCC
TLALAEHIAGNEDNFTQLMNEKAKQLKLSQPSPFLNATGMNNGTNKLSTTTAIEVGKLAT
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFSTNSNYS
HHHHCCHHHHHHHHHEEEEEEECCCEEEECCCEEEEECCCEEEEECCCCHHCCCCCCCCE
FVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWAVLLQ
EEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
FKNLIIQTLLIFFIITILMLLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TEYIVLEQLDKGNIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHALLLTGNNRS
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCC
TLALAEHIAGNEDNFTQLMNEKAKQLKLSQPSPFLNATGMNNGTNKLSTTTAIEVGKLAT
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFSTNSNYS
HHHHCCHHHHHHHHHEEEEEEECCCEEEECCCEEEEECCCEEEEECCCCHHCCCCCCCCE
FVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWAVLLQ
EEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
FKNLIIQTLLIFFIITILMLLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]