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The map label for this gene is moaA [H]

Identifier: 49184996

GI number: 49184996

Start: 1991729

End: 1992742

Strand: Direct

Name: moaA [H]

Synonym: BAS1985

Alternate gene names: 49184996

Gene position: 1991729-1992742 (Clockwise)

Preceding gene: 49184994

Following gene: 49184997

Centisome position: 38.09

GC content: 36.09

Gene sequence:

>1014_bases
ATGCACGAGAACATGAAAGATTCTTTAAAGCGGCCACTTCAAGATTTACGCATTTCAGTTATTGATCGTTGTAATTTTAG
GTGCACATATTGTATGCCTGCTGAAGTGTTCGGACCTGATTATGCTTTTTTACAGGAAGAGTGTTTATTAACGTTTGATG
AAATAGAAAGGTTAGCGAGATTATTTATAAGTATGGGAGTTAATAAAATTAGGCTTACTGGCGGTGAACCTTTATTGCGT
AAAGACTTACCGAAGTTAATTGCAAGACTTGCAAAGCTAGAAGGCTTAAAAGATATTGGGCTCACAACAAACGGAATTCA
TTTAGCAAAACAAGCTAAGGCGCTAAAAGAAGCAGGATTAAAGCGTGTAAATATTAGTTTAGATGCAATAGAGGATCGTG
TCTTCCAAAAAATTAATGGAAGACATGTAAGTACGAAACCTGTACTGAAAGGAATTGAAGCAGCGAAGGAAGCTGGATTA
GAAGTAAAGGTAAATATGGTCGTAAAAAAAGGGATGAATGATAGTCAAATTCTTCCTATGGCTCAGTATTTTAAAGAACA
AGAAATACAGTTACGTTTCATTGAGTTTATGGATGTAGGCAGTACGAACGGATGGAACTTTGAACAAGTCGTTACGAAGG
AACAATTAATAGAGAAAATTAATCGTGTATATCCAATTGAACCTGTTAAACCTCGTTACTTTGGAGAAGTTGCGAAATTG
TATCGATATGTAGGAAACGATGCTGAAGTTGGATTTATTACTTCAGTATCTGAGTCATTTTGTTCTTCTTGTACGAGAGC
ACGTATTTCGGCAGACGGCAAGTTTTATACGTGCTTATTTGGAGAAAAAGGAACGGATTTACGATCGCTTCTTCGAGAAA
ATATTTCTGATGCATCACTACTAAAAATTCTCCAACATACATGGGAGTTTAGAACAGATCGATATTCAGATGAAAGAACG
GCGGAAAGTACAAATAAACGTCAAAAAGTTGAGATGTCTTACATTGGTGGATAG

Upstream 100 bases:

>100_bases
TGAGAAAAGTCACATTGAATATGTAGCTTCTTTTTTATAATAAAGGCAAATAAAAAAATGAAAGCTTACTAAGGATTGCA
GCAGAAAAGAAGGGATATAG

Downstream 100 bases:

>100_bases
CGAAAGGAGGATTCCTTATGCAAGAGCGATATTCAAGACAAGTATTGTTTTCTGGAATAGGTGAAATGGGACAAAGGAAA
ATAAGGGAAAAGCATGTGCT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MHENMKDSLKRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEECLLTFDEIERLARLFISMGVNKIRLTGGEPLLR
KDLPKLIARLAKLEGLKDIGLTTNGIHLAKQAKALKEAGLKRVNISLDAIEDRVFQKINGRHVSTKPVLKGIEAAKEAGL
EVKVNMVVKKGMNDSQILPMAQYFKEQEIQLRFIEFMDVGSTNGWNFEQVVTKEQLIEKINRVYPIEPVKPRYFGEVAKL
YRYVGNDAEVGFITSVSESFCSSCTRARISADGKFYTCLFGEKGTDLRSLLRENISDASLLKILQHTWEFRTDRYSDERT
AESTNKRQKVEMSYIGG

Sequences:

>Translated_337_residues
MHENMKDSLKRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEECLLTFDEIERLARLFISMGVNKIRLTGGEPLLR
KDLPKLIARLAKLEGLKDIGLTTNGIHLAKQAKALKEAGLKRVNISLDAIEDRVFQKINGRHVSTKPVLKGIEAAKEAGL
EVKVNMVVKKGMNDSQILPMAQYFKEQEIQLRFIEFMDVGSTNGWNFEQVVTKEQLIEKINRVYPIEPVKPRYFGEVAKL
YRYVGNDAEVGFITSVSESFCSSCTRARISADGKFYTCLFGEKGTDLRSLLRENISDASLLKILQHTWEFRTDRYSDERT
AESTNKRQKVEMSYIGG
>Mature_337_residues
MHENMKDSLKRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEECLLTFDEIERLARLFISMGVNKIRLTGGEPLLR
KDLPKLIARLAKLEGLKDIGLTTNGIHLAKQAKALKEAGLKRVNISLDAIEDRVFQKINGRHVSTKPVLKGIEAAKEAGL
EVKVNMVVKKGMNDSQILPMAQYFKEQEIQLRFIEFMDVGSTNGWNFEQVVTKEQLIEKINRVYPIEPVKPRYFGEVAKL
YRYVGNDAEVGFITSVSESFCSSCTRARISADGKFYTCLFGEKGTDLRSLLRENISDASLLKILQHTWEFRTDRYSDERT
AESTNKRQKVEMSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=301, Percent_Identity=41.5282392026578, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI22027536, Length=301, Percent_Identity=41.5282392026578, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI1786999, Length=301, Percent_Identity=35.8803986710963, Blast_Score=200, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17561210, Length=332, Percent_Identity=34.9397590361446, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI32566331, Length=331, Percent_Identity=34.1389728096677, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32566329, Length=332, Percent_Identity=34.0361445783133, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI28574490, Length=314, Percent_Identity=37.2611464968153, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI28574488, Length=301, Percent_Identity=38.5382059800665, Blast_Score=208, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 38454; Mature: 38454

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHENMKDSLKRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEECLLTFDEIERLAR
CCCCHHHHHHCCHHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHH
LFISMGVNKIRLTGGEPLLRKDLPKLIARLAKLEGLKDIGLTTNGIHLAKQAKALKEAGL
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCC
KRVNISLDAIEDRVFQKINGRHVSTKPVLKGIEAAKEAGLEVKVNMVVKKGMNDSQILPM
EEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCHH
AQYFKEQEIQLRFIEFMDVGSTNGWNFEQVVTKEQLIEKINRVYPIEPVKPRYFGEVAKL
HHHHHHHHHHHHEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
YRYVGNDAEVGFITSVSESFCSSCTRARISADGKFYTCLFGEKGTDLRSLLRENISDASL
HHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHH
LKILQHTWEFRTDRYSDERTAESTNKRQKVEMSYIGG
HHHHHHHHHHHCCCCCCHHHHHHCCHHHEEEEHCCCC
>Mature Secondary Structure
MHENMKDSLKRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEECLLTFDEIERLAR
CCCCHHHHHHCCHHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHH
LFISMGVNKIRLTGGEPLLRKDLPKLIARLAKLEGLKDIGLTTNGIHLAKQAKALKEAGL
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCC
KRVNISLDAIEDRVFQKINGRHVSTKPVLKGIEAAKEAGLEVKVNMVVKKGMNDSQILPM
EEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCHH
AQYFKEQEIQLRFIEFMDVGSTNGWNFEQVVTKEQLIEKINRVYPIEPVKPRYFGEVAKL
HHHHHHHHHHHHEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
YRYVGNDAEVGFITSVSESFCSSCTRARISADGKFYTCLFGEKGTDLRSLLRENISDASL
HHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHH
LKILQHTWEFRTDRYSDERTAESTNKRQKVEMSYIGG
HHHHHHHHHHHCCCCCCHHHHHHCCHHHEEEEHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA