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The map label for this gene is narH [H]

Identifier: 49184989

GI number: 49184989

Start: 1985425

End: 1986900

Strand: Direct

Name: narH [H]

Synonym: BAS1978

Alternate gene names: 49184989

Gene position: 1985425-1986900 (Clockwise)

Preceding gene: 49184988

Following gene: 49184990

Centisome position: 37.97

GC content: 39.23

Gene sequence:

>1476_bases
TTGAAGATTAAAGCACAAGTCGGAATGGTAATGAACCTAGATAAATGCATCGGCTGCCATACTTGTAGCGTAACGTGCAA
AAACACCTGGACAAATCGTCCAGGTGCTGAATATATGTATTTCAATAACGTAGAAACAAAACCTGGTATTGGTTATCCGA
AGCAGTGGGAGGATCAGGAGAAATATAAAGGCGGCTGGGAATTGAAAAATGGTGAAATTCAGCTGAAATCCGGTTCGAAA
ATGAAGCGCCTTATGAATATTTTCCACAATCCAGATCAACCAACAATCGATGATTACTTTGAACCGTGGAACTATGATTA
TGAAACATTAACAACTAGTCCGCAGCGTAAGCATCAGCCAGTTGCTCGTCCAAAATCAGCAATTACAGGCGAATTTATCG
ACAAAATTGAATGGGGTCCAAACTGGGAAGATGATTTAGCTGGTGGACATATAACAGGATTACAAGATCCGAACGTAAAG
AAAATGGAAGAAGAAATTAAAACCGATTTTGAAAATGTTTTTATGATGTATTTACCACGCATATGCGAACATTGTATGAA
TCCATCTTGCGTATCGTCTTGTCCATCTGGTGCGATGTATAAACGGGAAGAAGATGGGATTGTTCTTGTAGATCAAAATG
CATGTCGTGCATGGAGATTCTGCGTATCGTCTTGCCCATATAAAAAAGTGTACGTTAACTGGCAAACGAATAAAGCGGAA
AAATGTACAATGTGTTTCCCGCGTATTGAAGCTGGTATGCCAACAATTTGTTCGGAAACATGTGTAGGGCGTATTCGGTA
TATTGGAGTAATGCTATATGACGCTGACAAGGTAAAAGAAGCGGCTTCTGTTGAGAATGAAAAAGATTTATATGAATCTC
AGCTTACTGTTTTCCTAGATCCAAATGATCCAGAAGTAGTAGCTGAAGCGAAAAAACAAGGCATTCCTGAAGAATGGATT
AAAGCGGCGCAAGAGTCGCCAATCTATAAAATGATTATCGATTGGAAAATTGCTCTGCCTCTTCATCCAGAGTATCGTAC
AATGCCGATGGTTTGGTATATCCCGCCGCTTAGCCCAATTATGAACATGGTGGAAGGGAAAGGTAGTAACTGGCAAGCAG
AAGAGATTTTCCCAGCTATTGATAATATGCGTATTCCAATTCAATATTTAGCGAATTTACTCACTGCAGGAGATGAATCA
CATATTCGACTTACATTGAAAAAAATGGCTGTTATGCGAACACATATGAGAGCACTGCAAATTAATAAAGAACCAAATGA
GGCTGTATTAAAAGAACTTGGCTTAACGAAACAGGATGTAGAAGATATGTATCGTCTTCTTGCTATCGCAAAATATAAAG
ATCGTTTTGTTATCCCGACGTCTCACCGCGAACAAGTTGCAGACTTATATAACGAACAAGGAAGCTGTGGTTTATCATTT
GCAGGTGGTCCAGGTTCTTGTATGACAATTTCTTAA

Upstream 100 bases:

>100_bases
GGATATGGTCAATTAAGTTATGGATTTAACTATTATGGTCCAACTGGGAACCAGCGCGATTTAAACGTTGTAATTCGCAA
ACTGAAGGAGGTAGATTGGC

Downstream 100 bases:

>100_bases
ATAATGGGGGCGGAAAAATATGAAACAAAGTCTACAAATTGCTTTTTCTTGTTCTTCATTCCTTCTCTCTTACCCGGAAC
TTGGGTGGAGAGAAGCTTTA

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTTSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYVNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVVAEAKKQGIPEEWI
KAAQESPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYNEQGSCGLSF
AGGPGSCMTIS

Sequences:

>Translated_491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTTSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYVNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVVAEAKKQGIPEEWI
KAAQESPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYNEQGSCGLSF
AGGPGSCMTIS
>Mature_491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTTSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYVNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVVAEAKKQGIPEEWI
KAAQESPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYNEQGSCGLSF
AGGPGSCMTIS

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=484, Percent_Identity=60.1239669421488, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=487, Percent_Identity=59.3429158110883, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=35.6060606060606, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=35.6060606060606, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=32.183908045977, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 56196; Mature: 56196

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQE
CEECCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
KYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTTSPQRKHQP
HHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHCCCCCHHHCCC
VARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYVNWQTNKAE
HHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCC
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLD
CHHEECHHHHCCCCHHHHHHHHHHEEEEEEEEECHHHHHHHHHCCCHHHHHHCCEEEEEC
PNDPEVVAEAKKQGIPEEWIKAAQESPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPI
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHH
MNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQ
HHHHCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHC
INKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYNEQGSCGLSF
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCCCEEEE
AGGPGSCMTIS
CCCCCCEEEEC
>Mature Secondary Structure
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQE
CEECCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
KYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTTSPQRKHQP
HHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHCCCCCHHHCCC
VARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYVNWQTNKAE
HHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCC
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLD
CHHEECHHHHCCCCHHHHHHHHHHEEEEEEEEECHHHHHHHHHCCCHHHHHHCCEEEEEC
PNDPEVVAEAKKQGIPEEWIKAAQESPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPI
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHH
MNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQ
HHHHCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHC
INKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYNEQGSCGLSF
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCCCEEEE
AGGPGSCMTIS
CCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]