Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 49184936

GI number: 49184936

Start: 1940301

End: 1941776

Strand: Direct

Name: gatA [H]

Synonym: BAS1925

Alternate gene names: 49184936

Gene position: 1940301-1941776 (Clockwise)

Preceding gene: 49184935

Following gene: 49184938

Centisome position: 37.11

GC content: 36.18

Gene sequence:

>1476_bases
ATGGAGATGCAGTTCAATACACTATTACAAAAAGAATTAACAATTCATGATATACAAACAGCGATGGAAGATGAAAAGTT
AACTTCAAAAGAATTAGTTATGTATTATCTTCATAGAATTGCAAAGTATGATCAAGACGGACCGAAAATTAATTCTATTT
TAGAAATAAATCCGGACGCTATTTTTATTGCAGAAGCGTTAGATCATGAAAGAAAGATTAAAGGTATAAGAGGTCCATTA
CATGGTATACCTGTTTTACTTAAAGACAATATTGAAACGAACGACTCTATGCATACAAGTGCAGGTACGATTGCTCTAGA
ACAACATATAAGTAGTGAAGATGCATTTCTCGTTACGAAGCTGAGAGAAGCAGGGGCAGTAATAATAGGAAAAGCAAATA
TGACAGAATTAGCAAATGGAATGTCTTTTGACATGTGGGCTGGATATAGTGCAAGAGGTGGGCAAACAATCAATCCTTAC
GGTACAGGTGAGGATGACATGTTTGTTGGAGGCTCAAGTACAGGGTCTGCCATAGCGGTTGCTGCTAACTTTACAGTAGT
ATCTGTTGGAACAGAAACAGATGGTTCTATATTGAGTCCAGCTGTTCAAAACTCTGTAGTAGGTATTAAACCGACTGTTG
GTTTAATTAGTCGTAGAGGTATTATTCCGTTCACATATTCGCAAGATACAGCCGGTCCATTTGCTAGAACCGTAACAGAT
GCAGCAATTTTACTAGGGAGTTTAACTGTGGTAGATGAGAAAGATGTAGCTACTCATAAAAGTAAAGGGATAGCAGAGCA
TGATTATACGAAATACCTTGATGTTAACGGTTTAAATGGAGCGAAGATTGGTGTATATAGCAATGCACCAAAAGAGTATT
ATGAATCTGGTGAGTATGATGAAAAATTGTTTAAGAAAACGATTGAGGTATTACGTAGTGAGGGAGCGACAGTAGTAGAA
GATATTGATATTCCGTCTTTTCATAGAGAATGGAGCTGGGGTGTATCGCTCTATGAATTAAAGCATAGTTTAGATAACTA
TCTTTCGAAATTACCTTCTACTATTCCAGTACATTCTATTTCAGAGTTAATGGAGTTTAATGAAAACATAGCAGAAAGAG
CTTTGAAATATGGACAAACTAAGTTGGAGAGAAGAAAAGATGTTCCTAATACGTTAAGAAATCCTGAATATATAAACGCA
AGATTAGAAGATATATATTTTTCTCAAGAACAAGGCATTGATTTTGCACTGGAAAAATATAATCTTGATGCGATTCTGTT
CCCTTCTTATATAGGCTCCACTATATGTGCGAAAGCAGGTTATCCGTCTATAGCAATACCTGCAGGGTATATGGAAGGTG
GAAGACCTTTTGGGATTACTCTTGCAAGTACAGCTTTTAGTGAAGGGACATTAATTAAACTAGCATACGCTTTTGAACAA
GCGACAAACCATAGAAAAATCCCGGGATTGTCATAA

Upstream 100 bases:

>100_bases
ATTCTTATAGAGGGATTGTAGTTGTATATGTGAGGAGCGGAGAAAATGAAAAAGTAATAGAGACATGCTATCAATAAAGG
GTTATAGAAGGAGAATGAAA

Downstream 100 bases:

>100_bases
GTAAAATATACAAAAAGGACAGGAATTCCTGTCCTTTTTGCTTTATTGATTACATTGAAATATTTTTTTGATCTGTTTTA
CGATATTTTAATAGGCTAAA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGIRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVIIGKANMTELANGMSFDMWAGYSARGGQTINPY
GTGEDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTVVDEKDVATHKSKGIAEHDYTKYLDVNGLNGAKIGVYSNAPKEYYESGEYDEKLFKKTIEVLRSEGATVVE
DIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDVPNTLRNPEYINA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
ATNHRKIPGLS

Sequences:

>Translated_491_residues
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGIRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVIIGKANMTELANGMSFDMWAGYSARGGQTINPY
GTGEDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTVVDEKDVATHKSKGIAEHDYTKYLDVNGLNGAKIGVYSNAPKEYYESGEYDEKLFKKTIEVLRSEGATVVE
DIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDVPNTLRNPEYINA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
ATNHRKIPGLS
>Mature_491_residues
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAEALDHERKIKGIRGPL
HGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVIIGKANMTELANGMSFDMWAGYSARGGQTINPY
GTGEDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
AAILLGSLTVVDEKDVATHKSKGIAEHDYTKYLDVNGLNGAKIGVYSNAPKEYYESGEYDEKLFKKTIEVLRSEGATVVE
DIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDVPNTLRNPEYINA
RLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
ATNHRKIPGLS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=26.2230919765166, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI195972892, Length=524, Percent_Identity=23.4732824427481, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=491, Percent_Identity=25.2545824847251, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=22.970297029703, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=415, Percent_Identity=27.710843373494, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=207, Percent_Identity=35.7487922705314, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648113, Length=471, Percent_Identity=26.963906581741, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21356731, Length=253, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648435, Length=256, Percent_Identity=28.125, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648437, Length=256, Percent_Identity=28.125, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45550774, Length=256, Percent_Identity=28.125, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24644968, Length=520, Percent_Identity=22.5, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=30.6818181818182, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=30.6818181818182, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24652985, Length=238, Percent_Identity=26.0504201680672, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19922090, Length=238, Percent_Identity=26.0504201680672, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24652981, Length=238, Percent_Identity=26.0504201680672, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24652983, Length=238, Percent_Identity=26.0504201680672, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 53933; Mature: 53933

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCE
IFIAEALDHERKIKGIRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTK
EEEEHHHCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEE
LREAGAVIIGKANMTELANGMSFDMWAGYSARGGQTINPYGTGEDDMFVGGSSTGSAIAV
HHHCCEEEEECCCHHHHHCCCCEEHCCCCCCCCCCEECCCCCCCCCEEECCCCCCCEEEE
AANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
EEEEEEEEECCCCCCCCCCHHHHCCCEECCCCHHHHHCCCCCEEEECCCCCCCHHHHHHH
AAILLGSLTVVDEKDVATHKSKGIAEHDYTKYLDVNGLNGAKIGVYSNAPKEYYESGEYD
HHHHHHCEEEECCCHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHCCCHH
EKLFKKTIEVLRSEGATVVEDIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSI
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SELMEFNENIAERALKYGQTKLERRKDVPNTLRNPEYINARLEDIYFSQEQGIDFALEKY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHEECHHCCCEEEEEEE
NLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
CCCEEEECHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHH
ATNHRKIPGLS
HCCCCCCCCCC
>Mature Secondary Structure
MEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCE
IFIAEALDHERKIKGIRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTK
EEEEHHHCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEE
LREAGAVIIGKANMTELANGMSFDMWAGYSARGGQTINPYGTGEDDMFVGGSSTGSAIAV
HHHCCEEEEECCCHHHHHCCCCEEHCCCCCCCCCCEECCCCCCCCCEEECCCCCCCEEEE
AANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTD
EEEEEEEEECCCCCCCCCCHHHHCCCEECCCCHHHHHCCCCCEEEECCCCCCCHHHHHHH
AAILLGSLTVVDEKDVATHKSKGIAEHDYTKYLDVNGLNGAKIGVYSNAPKEYYESGEYD
HHHHHHCEEEECCCHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHCCCHH
EKLFKKTIEVLRSEGATVVEDIDIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSI
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SELMEFNENIAERALKYGQTKLERRKDVPNTLRNPEYINARLEDIYFSQEQGIDFALEKY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHEECHHCCCEEEEEEE
NLDAILFPSYIGSTICAKAGYPSIAIPAGYMEGGRPFGITLASTAFSEGTLIKLAYAFEQ
CCCEEEECHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHH
ATNHRKIPGLS
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA