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The map label for this gene is ogt [H]

Identifier: 49184904

GI number: 49184904

Start: 1913341

End: 1913793

Strand: Direct

Name: ogt [H]

Synonym: BAS1892

Alternate gene names: 49184904

Gene position: 1913341-1913793 (Clockwise)

Preceding gene: 49184902

Following gene: 49184905

Centisome position: 36.59

GC content: 37.97

Gene sequence:

>453_bases
ATGTACCAAGCTTATTATGAAAGTGAATTAGGCTTGCTAGAAATTACAGCGAACGATAAAGGAATTACGTCTGTTATATT
TGTTGATGAGAGACAAGAAGAACATACAAATGAAATGATAGATCAATGTATAAATGAGCTCGATGAATATTTTAAGGGGA
ATAGAAAAGAGTTCACAGTCCCGTTGTCTCCTGAGGGAACAGCATTTCAAAAAAATGTATGGGATGCGCTCTATACGATT
CCTTATGGCGTAAGTGCTTCATATTTAGATATTGCAGAGAAAGTCGGAAATACGAAAGCTGTACGAGCAATAGGAGGAGC
AAATAGTCGCAATCCGATTTCAATAATCGTTCCGTGTCATCGTGTAATAGGAAAGAGCGGGAAACTTGTTGGGTATGCGG
GCGGTTTATGGAGGAAAGAGTGGTTATTAAAACACGAAGGTATTTTGAAATGA

Upstream 100 bases:

>100_bases
TATCATTCTCTTTTTTGTTTTCACTAATAAAACGCTTATACGAAAAATTTTGTCTATTCGTGTTACAATAACAAATATGA
ATTGAATTGGAGGATGACAT

Downstream 100 bases:

>100_bases
CGTATGGATGGGAAGAGGGATTGTTTTGAAATGTATAAATTGTGGGCAACTTTGCGAGGGAAATCAGTTAAATTGTGAAA
GTTGCCAGCGGACTTTGCAT

Product: methylated-DNA--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MYQAYYESELGLLEITANDKGITSVIFVDERQEEHTNEMIDQCINELDEYFKGNRKEFTVPLSPEGTAFQKNVWDALYTI
PYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEGILK

Sequences:

>Translated_150_residues
MYQAYYESELGLLEITANDKGITSVIFVDERQEEHTNEMIDQCINELDEYFKGNRKEFTVPLSPEGTAFQKNVWDALYTI
PYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEGILK
>Mature_150_residues
MYQAYYESELGLLEITANDKGITSVIFVDERQEEHTNEMIDQCINELDEYFKGNRKEFTVPLSPEGTAFQKNVWDALYTI
PYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCHRVIGKSGKLVGYAGGLWRKEWLLKHEGILK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=168, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=54.7169811320755, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1788542, Length=158, Percent_Identity=35.4430379746835, Blast_Score=100, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=108, Percent_Identity=44.4444444444444, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=108, Percent_Identity=44.4444444444444, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=40.7407407407407, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16841; Mature: 16841

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQAYYESELGLLEITANDKGITSVIFVDERQEEHTNEMIDQCINELDEYFKGNRKEFTV
CCCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PLSPEGTAFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCH
ECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECH
RVIGKSGKLVGYAGGLWRKEWLLKHEGILK
HHHCCCCCEEEECCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYQAYYESELGLLEITANDKGITSVIFVDERQEEHTNEMIDQCINELDEYFKGNRKEFTV
CCCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PLSPEGTAFQKNVWDALYTIPYGVSASYLDIAEKVGNTKAVRAIGGANSRNPISIIVPCH
ECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECH
RVIGKSGKLVGYAGGLWRKEWLLKHEGILK
HHHCCCCCEEEECCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]