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The map label for this gene is trsA [H]

Identifier: 49184852

GI number: 49184852

Start: 1868488

End: 1869726

Strand: Direct

Name: trsA [H]

Synonym: BAS1840

Alternate gene names: 49184852

Gene position: 1868488-1869726 (Clockwise)

Preceding gene: 49184851

Following gene: 49184853

Centisome position: 35.74

GC content: 35.59

Gene sequence:

>1239_bases
ATGCTAATTGTTAATAGAGAAGAGTATAGCAAAAGTGATTTTGATTTGAGATTACAAGCTTATGAGGAAATGGAACAATT
TCAAGAAGCAGCAGGAAATAGATTTGCACTTTGTCTGAAAGATCCATTCGATATTATTACGCTTGTGTTTTTCTTAAAAG
AAAAGAAATCATCCGTGTTACTTATACATGAAGATACGCCAAAAGAAACGGCTATTGAAATGGCGAAGCGTGCAAATTGT
ATCGGAATTTTATATGGAGAAAATAGCGATTTTACGAAATTAGAAGCAGTGAATTATTTAGCAGAAGAGCCTTCTTTATT
GCAATATAGTTCTGGAACTACAGGAGAACCGAAACTGATTCGTAGAGCATGGACGGAAGTTGATACAGAAATTAAGGTTT
ATAATGAAGCTTTAAACTGTGACATAGATGAAGTACCGATTGTTATGGCTCCTGTTTCACATTCATACGGACTAATATGT
GGTACGTTATCTGCAATTACGAGGGGAAGCAAACCTATCATCATTACGAATAAAAACCCTAAATTCGCATTAAATATAGT
TCGCAATACAGAAAAACATATCGTATATGCAGTACCACTTATGCTACATATTATGGGGAGTTTTCCGCAAGGAACGTTTC
AGTTTCATAAAATTATGACATCCGGAGCACCTTTGCCAGAAGCATTATTTTATAAACTAAAAGAAACGACAACATATATG
ATGCAACAATACGGTTGTTCTGAAGCAGGTTGTATTAGTATATGTCATGATATGAAAAGCCATTTAGATTTAGGAAACCC
GCTACCTCATGCGAGTATAAGTATAGGTTCAGATGAAAATGCACCGGAAGAAATCATAGTGAAAATGAATGATAAGGAAA
TTTTCACGAAAGATTTAGGGTATAAATCTGAGCGTGGGCTTCATTTTATGGGGCGTATGGACGATGTGATTAACGTTTCA
GGATTAAAAGTGTTTCCTATAGAGGTAGAAGAAACGATGCTTCGATTGGAAGGTGTGCAAGAGGCGATTGTATATCGAGG
GAAACATCCTGTCATGGGGGAAATTGTTAAAGCGAAAGTAATCTCTCATATTGATCCAGTTCAAATAAGAGAATGGTGTA
TGCAGCATTTACCATCTTATAAAGTTCCGCATGAGATTGAAAGTGTAACTGAAATTCCGAAAAATAAAACTGGAAAAGTA
AGTAGAAAGTTACTAGAGATGGGAGAGGTTACAACATGA

Upstream 100 bases:

>100_bases
TTGTATATAGATGTGGAATCGCTAGTTCATGAAGTTCCAAATCCATTGTATAGAGCAAGACAACTTAACATACAAAAATC
AGTTGCTACAGGGGGAAATT

Downstream 100 bases:

>100_bases
GACGGGAAGCGTTAAAGAATGCTGTATTAAAAATTATGACAGAAAAAATGGAACTGAAAAATGTAACGCATTTAGAAGAA
ACGATGCGTTTAAATCAAGA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: TrsI; AMP-binding ligase; Quinoxaline-2-carboxylic acid-activating enzyme [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MLIVNREEYSKSDFDLRLQAYEEMEQFQEAAGNRFALCLKDPFDIITLVFFLKEKKSSVLLIHEDTPKETAIEMAKRANC
IGILYGENSDFTKLEAVNYLAEEPSLLQYSSGTTGEPKLIRRAWTEVDTEIKVYNEALNCDIDEVPIVMAPVSHSYGLIC
GTLSAITRGSKPIIITNKNPKFALNIVRNTEKHIVYAVPLMLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYM
MQQYGCSEAGCISICHDMKSHLDLGNPLPHASISIGSDENAPEEIIVKMNDKEIFTKDLGYKSERGLHFMGRMDDVINVS
GLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKVISHIDPVQIREWCMQHLPSYKVPHEIESVTEIPKNKTGKV
SRKLLEMGEVTT

Sequences:

>Translated_412_residues
MLIVNREEYSKSDFDLRLQAYEEMEQFQEAAGNRFALCLKDPFDIITLVFFLKEKKSSVLLIHEDTPKETAIEMAKRANC
IGILYGENSDFTKLEAVNYLAEEPSLLQYSSGTTGEPKLIRRAWTEVDTEIKVYNEALNCDIDEVPIVMAPVSHSYGLIC
GTLSAITRGSKPIIITNKNPKFALNIVRNTEKHIVYAVPLMLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYM
MQQYGCSEAGCISICHDMKSHLDLGNPLPHASISIGSDENAPEEIIVKMNDKEIFTKDLGYKSERGLHFMGRMDDVINVS
GLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKVISHIDPVQIREWCMQHLPSYKVPHEIESVTEIPKNKTGKV
SRKLLEMGEVTT
>Mature_412_residues
MLIVNREEYSKSDFDLRLQAYEEMEQFQEAAGNRFALCLKDPFDIITLVFFLKEKKSSVLLIHEDTPKETAIEMAKRANC
IGILYGENSDFTKLEAVNYLAEEPSLLQYSSGTTGEPKLIRRAWTEVDTEIKVYNEALNCDIDEVPIVMAPVSHSYGLIC
GTLSAITRGSKPIIITNKNPKFALNIVRNTEKHIVYAVPLMLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYM
MQQYGCSEAGCISICHDMKSHLDLGNPLPHASISIGSDENAPEEIIVKMNDKEIFTKDLGYKSERGLHFMGRMDDVINVS
GLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKVISHIDPVQIREWCMQHLPSYKVPHEIESVTEIPKNKTGKV
SRKLLEMGEVTT

Specific function: Involved in triostin biosynthesis. Activates quinoxaline-2-carboxylic acid (QA) via catalysis of the ATP- pyrophosphate exchange reaction dependent on QA, and the formation of the corresponding adenylate. Also activates structural analogs of QA such as qu

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157311624, Length=368, Percent_Identity=25.5434782608696, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI157311622, Length=368, Percent_Identity=25.5434782608696, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI58082049, Length=368, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI115511026, Length=126, Percent_Identity=38.8888888888889, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI38505220, Length=361, Percent_Identity=24.0997229916898, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI28416953, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=348, Percent_Identity=26.1494252873563, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI221142682, Length=347, Percent_Identity=21.9020172910663, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI25141359, Length=320, Percent_Identity=28.4375, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=361, Percent_Identity=25.7617728531856, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=365, Percent_Identity=24.1095890410959, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994690, Length=364, Percent_Identity=23.9010989010989, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71994703, Length=364, Percent_Identity=23.9010989010989, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994694, Length=364, Percent_Identity=23.9010989010989, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=438, Percent_Identity=22.6027397260274, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 46510; Mature: 46510

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVNREEYSKSDFDLRLQAYEEMEQFQEAAGNRFALCLKDPFDIITLVFFLKEKKSSVL
CEEECCHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCEE
LIHEDTPKETAIEMAKRANCIGILYGENSDFTKLEAVNYLAEEPSLLQYSSGTTGEPKLI
EEECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHH
RRAWTEVDTEIKVYNEALNCDIDEVPIVMAPVSHSYGLICGTLSAITRGSKPIIITNKNP
HHHHHHHCCHHEEEHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCC
KFALNIVRNTEKHIVYAVPLMLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYM
CEEEEEEECCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MQQYGCSEAGCISICHDMKSHLDLGNPLPHASISIGSDENAPEEIIVKMNDKEIFTKDLG
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHCC
YKSERGLHFMGRMDDVINVSGLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKV
CCCCCCEEECCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ISHIDPVQIREWCMQHLPSYKVPHEIESVTEIPKNKTGKVSRKLLEMGEVTT
HHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLIVNREEYSKSDFDLRLQAYEEMEQFQEAAGNRFALCLKDPFDIITLVFFLKEKKSSVL
CEEECCHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCEE
LIHEDTPKETAIEMAKRANCIGILYGENSDFTKLEAVNYLAEEPSLLQYSSGTTGEPKLI
EEECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHH
RRAWTEVDTEIKVYNEALNCDIDEVPIVMAPVSHSYGLICGTLSAITRGSKPIIITNKNP
HHHHHHHCCHHEEEHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCC
KFALNIVRNTEKHIVYAVPLMLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYM
CEEEEEEECCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MQQYGCSEAGCISICHDMKSHLDLGNPLPHASISIGSDENAPEEIIVKMNDKEIFTKDLG
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHCC
YKSERGLHFMGRMDDVINVSGLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKV
CCCCCCEEECCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ISHIDPVQIREWCMQHLPSYKVPHEIESVTEIPKNKTGKVSRKLLEMGEVTT
HHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA