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The map label for this gene is ldh

Identifier: 49184796

GI number: 49184796

Start: 1809442

End: 1810386

Strand: Reverse

Name: ldh

Synonym: BAS1784

Alternate gene names: 49184796

Gene position: 1810386-1809442 (Counterclockwise)

Preceding gene: 49184799

Following gene: 49184789

Centisome position: 34.62

GC content: 37.46

Gene sequence:

>945_bases
ATGAAAAAAGGTATTAACCGTGTTGTATTAGTAGGAACAGGAGCAGTTGGATGTAGTTATGCTTACTGCATGATTAACCA
AGCTGTAGCTGAAGAATTTGTTTTAGTTGATGTAAACGAAGCAAAAGCTGAAGGAGAAGCGATGGATTTAAGTCATGCCG
TTCCATTTGCTCCAGCTCCAACTAGAGTATGGAAAGGTAGCTACGAAGATTGTAAAGATGCTGACCTTGTAGTCATTACG
GCTGGATTACCACAAAAGCCAGGCGAAACACGTTTAGATTTAGTTGAGAAAAATGCAAAAATCTTTAAACAAATCGTGCG
CAGTATTATGGATAGCGGATTTGATGGTATCTTCTTAATCGCAACTAACCCTGTCGATATTTTAACTTACGTAACTTGGA
AAGAATCTGGTTTACCGAAAGAACGCGTAATTGGTTCTGGCACAACACTTGATTCTGCTCGTTTCCGCTATATGTTAGGT
GAGTACTTCAATATTGGTCCACACAACATTCATGCTTATATTATCGGAGAACATGGTGATACGGAACTTCCTGTTTGGAG
TCACGTATCAGTTGGTATTCAAAAACTACAAACACTGCTTGAAAAAGACAATACGTATAATCAAGAAGATTTAGACAAAA
TCTTCATAAACGTTCGTGATGCAGCTTACCATATTATTGAACGAAAAGGTGCAACATACTATGGTATTGGTATGTCACTT
CTACGCGTTACTAAGGCAATTTTAAACGACGAAAATAGTGTATTAACTGTTTCGGCATATTTAGAAGGACAATACGGTCA
AAAAGATGTGTATATCGGTGTACCTGCTGTTTTAAATCGCGGCGGTGTGCGTGAAATTTTAGAAGTAGAATTAAGTGAAG
ACGAAGAATTAAAATTTGATCACTCAGTTCAAGTGTTGAAAGAAACAATGGCTCCTGTTCTTTAA

Upstream 100 bases:

>100_bases
GAATATCTTTTATAAAATGTAATAAACTAGATATGAATCTATAGAAGTAAGACGTATTATCTTACATAATAAAATCAATG
AAATTGCGAGGAATTTTGAA

Downstream 100 bases:

>100_bases
TCATATATGTGAAAAAGCAATCCAATTTATTTTGGATTGCTTTTTTGTTTATTTTCTATTTTTTAATCGATTCCTCATTA
TTTACATCAAGTACATCTAA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 1

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAPTRVWKGSYEDCKDADLVVIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
EYFNIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFDHSVQVLKETMAPVL

Sequences:

>Translated_314_residues
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAPTRVWKGSYEDCKDADLVVIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
EYFNIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFDHSVQVLKETMAPVL
>Mature_314_residues
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAPTRVWKGSYEDCKDADLVVIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
EYFNIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFDHSVQVLKETMAPVL

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=41.6393442622951, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=41.6393442622951, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI5031857, Length=303, Percent_Identity=40.9240924092409, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI260099723, Length=303, Percent_Identity=40.9240924092409, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=40.2640264026403, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=40.2640264026403, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI9257228, Length=302, Percent_Identity=41.3907284768212, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI4504973, Length=302, Percent_Identity=41.3907284768212, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=38.3606557377049, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI260099727, Length=194, Percent_Identity=47.4226804123711, Blast_Score=187, Evalue=8e-48,
Organism=Homo sapiens, GI260099725, Length=194, Percent_Identity=47.4226804123711, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI207028494, Length=204, Percent_Identity=39.7058823529412, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI103472011, Length=293, Percent_Identity=25.938566552901, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=28.6486486486486, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI21735621, Length=318, Percent_Identity=24.5283018867925, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1789632, Length=324, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535107, Length=294, Percent_Identity=41.156462585034, Blast_Score=227, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17554310, Length=318, Percent_Identity=27.9874213836478, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322765, Length=193, Percent_Identity=30.5699481865285, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17136226, Length=308, Percent_Identity=38.961038961039, Blast_Score=235, Evalue=2e-62,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=28.9902280130293, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24647881, Length=305, Percent_Identity=27.2131147540984, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24663599, Length=326, Percent_Identity=23.0061349693252, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH1_BACAN (Q81RW4)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_844332.1
- RefSeq:   YP_018568.2
- RefSeq:   YP_028048.1
- ProteinModelPortal:   Q81RW4
- SMR:   Q81RW4
- EnsemblBacteria:   EBBACT00000013196
- EnsemblBacteria:   EBBACT00000013940
- EnsemblBacteria:   EBBACT00000021770
- GeneID:   1086286
- GeneID:   2816222
- GeneID:   2850587
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_1923
- KEGG:   bar:GBAA_1923
- KEGG:   bat:BAS1784
- TIGR:   BA_1923
- TIGR:   GBAA_1923
- GeneTree:   EBGT00050000001153
- HOGENOM:   HBG566126
- OMA:   KVVDSAY
- ProtClustDB:   PRK00066
- BioCyc:   BANT260799:BAS1784-MONOMER
- BioCyc:   BANT261594:GBAA1923-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34788; Mature: 34788

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00064 L_LDH; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 178-178 BINDING 91-91 BINDING 123-123 BINDING 154-154 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAP
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
TRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLI
CHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFNIGPHNIHAYIIGEHGD
ECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCC
TELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEHHHHHHHHHHHCCCEEEHHHHHH
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFD
HHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECHHHHCCCCHHHHHEEECCCCCCCCCH
HSVQVLKETMAPVL
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAP
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
TRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLI
CHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFNIGPHNIHAYIIGEHGD
ECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCC
TELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEHHHHHHHHHHHCCCEEEHHHHHH
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFD
HHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECHHHHCCCCHHHHHEEECCCCCCCCCH
HSVQVLKETMAPVL
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629