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The map label for this gene is gatA [H]

Identifier: 49184794

GI number: 49184794

Start: 1806584

End: 1808194

Strand: Direct

Name: gatA [H]

Synonym: BAS1782

Alternate gene names: 49184794

Gene position: 1806584-1808194 (Clockwise)

Preceding gene: 49184793

Following gene: 49184795

Centisome position: 34.55

GC content: 35.57

Gene sequence:

>1611_bases
ATGAAGAAATGGGTGAAGGTAACGCTGTCTATCGCTGGAGGTATTGTCTTATTAGCTTGTGCCGGCGGATATTACGTGTA
TAAAAATTATTTTCCAAAGGAACCTGAGCGTATTGTATATGATAAAGAAAGAGTGCTACAACCGATACATAATCAGCTTA
AAGGAATCAATATAGAAAATGTAAAGATAAAAGAAAAAGAAGTTGTAAATGCGACTGTAGATGAGCTTCAAAAAATGATT
GATGATGGAAAATTGTCATATGAAGAATTAACGAGTATTTATCTCTTTAGAATACAAGAACATGATCAAAATGGAATAAC
ATTAAATTCTGTTACAGAGATAAATCCTAATGCGATGGAAGAAGCAAGAAAGTTAGATCAAGAGCGAAGTAGAAACAAAA
AGTCGAATTTATATGGTATTCCTGTCGTTGTAAAAGATAATGTACAAACGGCAAAAGTGATGCCGACTAGTGCAGGAACT
TATGTATTAAAAGATTGGATTGCAGATCAAGACGCAACAATTGTGAAGCAGTTGAAAGAAGAAGGGGCATTTGTTTTAGG
AAAAGCGAATATGTCTGAGTGGGCGAATTATTTATCTTTTACAATGCCAAGCGGGTATAGCGGGAAGAAAGGACAAAATT
TGAATCCATACGGTCCGATTATGTTTGATACATCGGGATCAAGTTCAGGATCTGCTACAGTAGTTGCAGCAGATTTTGCA
CCGCTTGCAGTCGGAACAGAAACGACAGGATCGATTGTAGCACCCGCAGCGCAGCAATCAGTAGTTGGGTTACGTCCATC
TTTAGGTAGGGTAAGTAGAACAGGAATTATTCCGTTAGCTGAAACTCTTGATACAGCAGGACCTATGGCAAGAACGGTAA
AAGATGCAGCAACGTTATTTAATGCAATGATAGGTTATGACGAAAAAGATGTTATGACAGAAAAAGTGAAAGATAAAGAA
AGAATTGATTATACAAAGGATTTATCAATAGATGGATTAAAAGGGAAAAAGATAGGGCTTCTTTTTTCTGTAGACCAACA
AGACGAAAATAGAAAAGCAGTAGCAGAAAAGATTAGAAAAGACCTTCAAGATGCAGGTGCGATATTAACTGATTATATTC
AGTTAAACAATGGGGGTGTAGATAATTTACAAACATTAGAATATGAGTTCAAGCATAATGTAAACGACTATTTTTCACAG
CAAAAGAATGTACCTGTAAAATCGTTAAAAGAGATTATAGCGTTTAATAAAAGAGATAGTAATAGACGAATAAAATATGG
ACAAACATTAATTGAGGCGTCTGAAAAATCTACTATAACGAAAGATGAGTTTGAAAAAGTAGTGCAAACGAGTCAAGAAA
ATGCAAAAAAAGAGCTGAATAAATATTTAGTAGAAAAGGGTTTAGACGCTTTAGTTATGATTAACAATGAGGAAGTTCTT
CTATCCGCTGTAGCTGGCTATCCAGAATTAGCGGTGCCAGCAGGATATGATAACAATGGAGAGCCAGTAGGTGCAGTATT
TGTCGGGAAACAATTCGGTGAGAAGGAACTGTTCAATATTGGATATGCGTATGAACAGCAGTCTAAAAATAGAAAACCAC
CTAAATTGTAA

Upstream 100 bases:

>100_bases
TCTTTCATTTTTTCATTTAGCATTTTGATTGGAGAGAGTTAAGTAAAAGGTAACTCTCTTTTTCGTATAAAGAAGCACCT
TATAGAGTGGAGGAAGCAAC

Downstream 100 bases:

>100_bases
AAGGTTAAACGAAAAAAAGAGCTCAACTGAAGTAAGTAGGTGAGCTAATATAAATAGAATCTAACAATATAAAAAGAAAA
AAAGGATTCTTCCTAATTAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKKWVKVTLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVAADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGRVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLKEIIAFNKRDSNRRIKYGQTLIEASEKSTITKDEFEKVVQTSQENAKKELNKYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL

Sequences:

>Translated_536_residues
MKKWVKVTLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVAADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGRVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLKEIIAFNKRDSNRRIKYGQTLIEASEKSTITKDEFEKVVQTSQENAKKELNKYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
>Mature_536_residues
MKKWVKVTLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATVDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVAADFA
PLAVGTETTGSIVAPAAQQSVVGLRPSLGRVSRTGIIPLAETLDTAGPMARTVKDAATLFNAMIGYDEKDVMTEKVKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQ
QKNVPVKSLKEIIAFNKRDSNRRIKYGQTLIEASEKSTITKDEFEKVVQTSQENAKKELNKYLVEKGLDALVMINNEEVL
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=258, Percent_Identity=32.5581395348837, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI195972892, Length=247, Percent_Identity=32.7935222672065, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=235, Percent_Identity=29.7872340425532, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=520, Percent_Identity=21.9230769230769, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=275, Percent_Identity=24.3636363636364, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=224, Percent_Identity=36.1607142857143, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319685, Length=187, Percent_Identity=31.5508021390374, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=25.3493013972056, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=24.031007751938, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356731, Length=277, Percent_Identity=24.187725631769, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=29.4354838709677, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=29.4354838709677, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=29.4354838709677, Blast_Score=76, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 59474; Mature: 59474

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWVKVTLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIEN
CCCEEEEEEEHHCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATVDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEECEECCCCHHHH
EARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVAADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
PLAVGTETTGSIVAPAAQQSVVGLRPSLGRVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NAMIGYDEKDVMTEKVKDKERIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQQKNVPVKSLKEIIAFNKRDS
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCC
NRRIKYGQTLIEASEKSTITKDEFEKVVQTSQENAKKELNKYLVEKGLDALVMINNEEVL
CCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
EHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKWVKVTLSIAGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIEN
CCCEEEEEEEHHCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATVDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEECEECCCCHHHH
EARKLDQERSRNKKSNLYGIPVVVKDNVQTAKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPIMFDTSGSSSGSATVVAADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
PLAVGTETTGSIVAPAAQQSVVGLRPSLGRVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NAMIGYDEKDVMTEKVKDKERIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTDYIQLNNGGVDNLQTLEYEFKHNVNDYFSQQKNVPVKSLKEIIAFNKRDS
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCC
NRRIKYGQTLIEASEKSTITKDEFEKVVQTSQENAKKELNKYLVEKGLDALVMINNEEVL
CCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDNNGEPVGAVFVGKQFGEKELFNIGYAYEQQSKNRKPPKL
EHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA