Click here to switch to the map view.

The map label for this gene is asnO [H]

Identifier: 49184639

GI number: 49184639

Start: 1641221

End: 1643044

Strand: Reverse

Name: asnO [H]

Synonym: BAS1625

Alternate gene names: 49184639

Gene position: 1643044-1641221 (Counterclockwise)

Preceding gene: 49184642

Following gene: 49184637

Centisome position: 31.42

GC content: 36.73

Gene sequence:

>1824_bases
ATGTGTGGAATTACAGGTTGGGTAGATTGGAAAGAAGACCTTTCAAATCAACATGTTATTTTAAAAAAGATGGCAAATCG
TATTCAGCATCGCGGTCCTGATGCTGAAGGATTTTGGTTTTCACCTCGTGCGGCCTTTGCACACCGTCGTTTAATTGTAA
TTGATCCAGAAGGCGGGGCACAGCCGAAGACATTTCGTGCTGGTGATTATACGTATGCTCTTACTTATAATGGAGAAATT
TATAATTTCCGTGAGCTGAGGGAACAACTTCAAAAATGTGGTCATACATTTGAAACGCATTCAGATACAGAAGTGTTACT
ACATGCTTATTTAGAATGGAAAGAAGACTGCGTGCAACATTTAAACGGCATTTTCGCTTTCGCCTTATGGGATGATCAGA
AGCAACGACTGTTTTTAGCACGTGATCATTTAGGTGTAAAACCGCTCTTTTATACAGAAAGAAATGACAGTATTATTTTC
GGATCTGAAATAAAAGCATTATTAGCTCATCCATCTGTTCCTGCTGAAATTGATGCGGATGGCATAAATGAAATATTTGG
ATTAGGGCTATTTCGAACTCCAGGATGTGGCGTCTTTAAACATATTGAAGAAGTGCGTGCCGGACATTCTATCACATTTA
CACGTGATAAAAAAGTAGTTACGAAGTATTGGAATTTAGAAAGTAAGGTCCATACAGATTCTATAGAAGATACATCATCA
CATATTTTATCTATTTTACAAGATACAGTAAAAAGACAATTAATTGCCGACGTCCCTCTCGTTTGCATGTTATCAGGAGG
ATTAGATTCTAGCGGTATTACTGCACTAGCAGGAAAAGAATTTGCTGCTGAAAATAAAACACTTCATACGTATTCCGTTG
ACTTTGTAAATAGCGCAAAAGATTTCGAGCTGACATTTGCTCGCACTGGTTTAGACGCTCCTTGGGTAAAACGCGTTTCT
GAACATGTAGGGACATCTCATCATGATATTATTGTGAATGCTGAAGAATTAGCTAATCACTTATTCGTTCCCCTCCGTGC
AAAAGATTTACCGAGTGCTGGCGAAATGGAAACTTCACTATATTTACTATTTTGCGAAATGAAAAAAGATGCGACCGTAG
CTTTATCCGGCGAATCTGCTGATGAAGTATTCGGTGGATACCCTTGGTTTCATCAAGAAGAATTACTATATGTCGATAAG
TTTCCTTGGCTAACAAATTGGAAGAATACATCTCCTCTTCTACTACATGAAATAAGTAACCAATGTAATCTAGAAAACTA
TATTGATACACGCTTCCAAGAAGCGATACTTGAAGTACCTACTCTTGAAGGCGAAAGTAAAAAATCCGCGAAACAACGTC
AAATGTTTTATTTATTTTTAACGAGATTTCTTCCGTTCTTACTTGACCGTAAAGATCGTATGAGTATGGCTGTAGGATTT
GAAGTTCGTGTACCGTTTTGCGATTATCGATTAGTTGAATATTTATGGAACGTTCCTTTCAACATAAAAAGCATTGATAA
TATTGAAAAAGGTATTTTACGTAGAGCACTGAAACCTGCTTTACCTGACGATGTACGTAATAGAAGAAAAAGTGCGTATC
CAACTTCACAAGACCCACAGTATTTACAAACAATCCGTCATTTATCACTCGACATGTGCAGTAATAAAAACAACCCTATT
TTCTCACTTATTAATCATTCCACATTACTTGCCATTGCCGATGGAAGTAATAAAGAAATTAACAATTTCGAAGCAAGAAG
TGCTATGGAATACATGCTACAAGTAAATGAATGGTTAAAAACATATCATATTCATATTGCTTAA

Upstream 100 bases:

>100_bases
AATTTGATTCATGTAGTACACTATTTATATAGTAACTCTTTAACTTAGTGTACATTTTATTTCATTAATCTACTATTTTA
AATTAGGAGATGAGATGTGT

Downstream 100 bases:

>100_bases
CAAAAAGGAGTTCAAGGATAAAAACCTTGAACTCCTTTTATATTAATTGGCACATCGTTTATTTTCAGTGTCAATGGACC
ATCCAATAATTGTGGCGCAA

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREQLQKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDADGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEISNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPQYLQTIRHLSLDMCSNKNNPI
FSLINHSTLLAIADGSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA

Sequences:

>Translated_607_residues
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREQLQKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDADGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEISNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPQYLQTIRHLSLDMCSNKNNPI
FSLINHSTLLAIADGSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA
>Mature_607_residues
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREQLQKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDADGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEISNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPQYLQTIRHLSLDMCSNKNNPI
FSLINHSTLLAIADGSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=554, Percent_Identity=27.9783393501805, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI168229252, Length=554, Percent_Identity=27.9783393501805, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI168229250, Length=554, Percent_Identity=27.9783393501805, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI296010848, Length=528, Percent_Identity=27.8409090909091, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI296010852, Length=454, Percent_Identity=26.2114537444934, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI296010850, Length=454, Percent_Identity=26.2114537444934, Blast_Score=123, Evalue=5e-28,
Organism=Escherichia coli, GI1786889, Length=411, Percent_Identity=31.1435523114355, Blast_Score=162, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI25147557, Length=545, Percent_Identity=26.7889908256881, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71993933, Length=545, Percent_Identity=26.7889908256881, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI25147560, Length=321, Percent_Identity=26.791277258567, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321563, Length=431, Percent_Identity=28.7703016241299, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325403, Length=420, Percent_Identity=29.7619047619048, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI45553209, Length=378, Percent_Identity=28.8359788359788, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69558; Mature: 69558

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGA
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELREQLQKCGHTFETHSDTEVLLHAYLEWKEDCVQH
CCCEEECCCEEEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIFGSEIKALLAHPSVPAEIDAD
HCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCCCCCC
GINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTGLDAPWVKRVSEHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSL
CEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHEEEEEECCCCCCCCCCCCEE
YLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDKFPWLTNWKNTSPLLLHEISN
EEEEEECCCCCEEEECCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCC
QCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEE
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPQ
EEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHH
YLQTIRHLSLDMCSNKNNPIFSLINHSTLLAIADGSNKEINNFEARSAMEYMLQVNEWLK
HHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
TYHIHIA
HEEEEEC
>Mature Secondary Structure
MCGITGWVDWKEDLSNQHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGA
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELREQLQKCGHTFETHSDTEVLLHAYLEWKEDCVQH
CCCEEECCCEEEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIFGSEIKALLAHPSVPAEIDAD
HCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCCCCCC
GINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTGLDAPWVKRVSEHVGTSHHDIIVNAEELANHLFVPLRAKDLPSAGEMETSL
CEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHEEEEEECCCCCCCCCCCCEE
YLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDKFPWLTNWKNTSPLLLHEISN
EEEEEECCCCCEEEECCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCC
QCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEE
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPQ
EEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHH
YLQTIRHLSLDMCSNKNNPIFSLINHSTLLAIADGSNKEINNFEARSAMEYMLQVNEWLK
HHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
TYHIHIA
HEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]