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The map label for this gene is ansA [H]

Identifier: 49184423

GI number: 49184423

Start: 1434140

End: 1435114

Strand: Reverse

Name: ansA [H]

Synonym: BAS1406

Alternate gene names: 49184423

Gene position: 1435114-1434140 (Counterclockwise)

Preceding gene: 49184427

Following gene: 49184409

Centisome position: 27.45

GC content: 37.03

Gene sequence:

>975_bases
TTGAAAAGAATCCTAGTTTTACACACAGGTGGAACAATTGCAATGGAGGAAGATAAAGAAACTGGGGTCGTACAACCAGG
TGAAAAAAATCCTCTTTTAAAATTCATTCCTGATTTAGAAGGCGATGTTGATTTAATCGTTGAAGATGTGTTCCATCTAC
CATCTCCTCATATGACACCAAGCGAAATGTTACAATTACAAGTCATCATTGATGAAAGAGTAAAACAAGACAACATTCAT
GGCGTAGTCATTACGCATGGTACGGATACATTAGAAGAAACAGCCTATTTCTTAGATTTAACAGTACAAGCAACTATTCC
GATCGTTGTAACAGGTGCAATGCGATCTAGTAATGAATTAGGTGCTGATGGTCTATATAACTTCTTATCAGCTGTAAAAG
TCGCTAGCAGTAGTGAAGCCGCAGAAAAAGGTGTTTTAGTCGTATTAAACGACGAAATTCATTGCGCTACAAACGTAACG
AAAACACATACAAGCAATGTAGCAACATTCCAAAGTCCACAGTATGGACCAATTGGTATGGTAACAAAACGCGGTGTTGT
ATTCCACCATGCTTTAGTACATCACGAAACATATTCAGTAAATAAGGTTTCTAAAAACGTAGTCGTTCTAAAAGCATACG
CAGGCATGGACGATACATTGTTAGCAGCAATTGAAAACCTTCCAGTAGACGGGATTGTTATTGAAGCACTCGGACAAGGT
AATTTACCTCCAAGAACACTTCCTAGTCTAGAACGATTAATAAACAAAGGAATTCCTGTCGTATTAGTTTCTCGCTGTTT
CAACGGCATCGTTCAAGATGTATATTCATATGAAGGCGGCGGCAAACAATTAAAAGATATGGGTATTGTATTCACATACG
GTTTAAATGCTCAAAAAGCACGTATTAAATTATTAGTTGCATTAGAGAATACAACTTCTCATGAAGCAATCCAAAATATG
TTTATGCACGATTAA

Upstream 100 bases:

>100_bases
TTGTCATTTTCGAGGCATACTTTTATACATTTCCATTGTCGAATTTACTCAAAACATGTTATGTTCTAAATGTATTTTTT
ACTTGAAAGGGGTATAAGCA

Downstream 100 bases:

>100_bases
AAAAGAAGCTGTCGAAGTTTATTTCGACAGCTTCTTTTTATATTTCTCTTGTCGCAATGGTTTGCGCAATTGCATCTCCA
TGAAATCGACCGTTCTCAAT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKRILVLHTGGTIAMEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIH
GVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVT
KTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVHHETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQG
NLPPRTLPSLERLINKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHEAIQNM
FMHD

Sequences:

>Translated_324_residues
MKRILVLHTGGTIAMEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIH
GVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVT
KTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVHHETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQG
NLPPRTLPSLERLINKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHEAIQNM
FMHD
>Mature_324_residues
MKRILVLHTGGTIAMEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIH
GVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVT
KTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVHHETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQG
NLPPRTLPSLERLINKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKARIKLLVALENTTSHEAIQNM
FMHD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=313, Percent_Identity=27.4760383386581, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789327, Length=299, Percent_Identity=36.1204013377926, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1788065, Length=289, Percent_Identity=26.2975778546713, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=340, Percent_Identity=25.2941176470588, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17558280, Length=340, Percent_Identity=25.2941176470588, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=340, Percent_Identity=25.2941176470588, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI193207374, Length=340, Percent_Identity=25.2941176470588, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=338, Percent_Identity=29.2899408284024, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323187, Length=338, Percent_Identity=29.2899408284024, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323186, Length=338, Percent_Identity=29.2899408284024, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323184, Length=338, Percent_Identity=29.2899408284024, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320527, Length=333, Percent_Identity=29.1291291291291, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24645530, Length=342, Percent_Identity=26.3157894736842, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24639609, Length=341, Percent_Identity=23.1671554252199, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35284; Mature: 35284

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVLHTGGTIAMEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTP
CCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCH
SEMLQLQVIIDERVKQDNIHGVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNEL
HHHEEEEEEEHHHCCCCCCCEEEEECCCCCHHHCEEEEEEEEEEEEEEEEEECCCCCCCC
GADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQYGPIGM
CHHHHHHHHHHHHHCCCCCHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCCCE
VTKRGVVFHHALVHHETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQG
EECCCCCEEHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCC
NLPPRTLPSLERLINKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKA
CCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCE
RIKLLVALENTTSHEAIQNMFMHD
EEEEEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKRILVLHTGGTIAMEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTP
CCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCH
SEMLQLQVIIDERVKQDNIHGVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNEL
HHHEEEEEEEHHHCCCCCCCEEEEECCCCCHHHCEEEEEEEEEEEEEEEEEECCCCCCCC
GADGLYNFLSAVKVASSSEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQYGPIGM
CHHHHHHHHHHHHHCCCCCHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCCCE
VTKRGVVFHHALVHHETYSVNKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQG
EECCCCCEEHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCC
NLPPRTLPSLERLINKGIPVVLVSRCFNGIVQDVYSYEGGGKQLKDMGIVFTYGLNAQKA
CCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCE
RIKLLVALENTTSHEAIQNMFMHD
EEEEEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]