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The map label for this gene is pit [H]

Identifier: 49184378

GI number: 49184378

Start: 1390810

End: 1391808

Strand: Reverse

Name: pit [H]

Synonym: BAS1361

Alternate gene names: 49184378

Gene position: 1391808-1390810 (Counterclockwise)

Preceding gene: 49184379

Following gene: 49184377

Centisome position: 26.62

GC content: 37.24

Gene sequence:

>999_bases
ATGGATACACTCTTGATACTGACCGTTTTAGTAGTCATTTGCGCTTTAGCTTTTGACTTTATCAATGGTTTTCACGATAC
AGCAAACGCTATTGCAACGGCTGTTTCAACGAAAGCTCTAAAGCCTAGACATGCCATTGTTATGGCGGCTATTATGAACT
TTTTAGGTGCAATGACATTTACAGGTGTAGCAAAAACGATTACAAAAGATATCGTTGATCCATTCGCCTTACAAAATGGT
TCTCTTGTAATTTTAGCTGCTTTGCTTGCAGCAATAGCTTGGAACTTAATTACTTGGTACTACGGTATTCCAAGTAGTTC
TTCGCATGCAATTATCGGCTCAATCGCAGGTGCAGCAATTGCTGCCGCTGGGATTAGCTCGCTAAATCTTAAAGGATTCA
TTAAAATTATTGAAGCTTTAATCATTTCACCAATTATCGCTTTCGTTATTGGTTATACCGTATACAGTATTTTTAAAGTA
GTATTTAAAAACTTTAACTTAACGAAAACGAATAAGAATTTCCGTATATTCCAGATTTTCACTGCTGCATTACAGGCTTA
TACACATGGTACGAATGATGCGCAAAAAGCAATGGGAATCATTACGATGGCTCTTATGGCTAATAACTATCATACTTCGA
GCGACATCCCGTTCTGGGTACAATTATCTTGTGCAATTGCAATGGGTCTTGGTACTTCTGTCGGTGGATGGAAAATTATT
AAAACTGTCGGTGGACAAATCATGAAAATTCGTCCTGTAAATGGTGTAGCTGCTGATTTATCATCATCTCTTGTTATTTT
CGGGGCAACATTTATTCACTTACCAGTTAGTACGACGCACGTTATCTCCTCTTCTATTTTAGGGGTTGGTGCTTCTCATC
GTGTAAAAGGTGTAAAATGGGGAACTGCAAAACGCATGTTAATTACTTGGGTTATCACACTTCCTATTTCAGCTTCATTA
GCTGCTTTATTTTACTCTTTATTACACTTAATCTTTTAA

Upstream 100 bases:

>100_bases
AAGAGATTTACGAAGAGCTTGAAGAAGTAGCGGATAGCTGCCAAAGCGTTGCAAACGTATTAGAAACAATTATTATGAAG
AACGCGTAAGGAGTTTGATC

Downstream 100 bases:

>100_bases
TAAAAAGTCGTCTTCCTTATTTTTCTAGGAAGACGACTTTTTTGTGAACAAAGCTTTTTCTCATGAAAAAGCGTGTATAA
TAATTGGTGGATAAGTTATA

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIVMAAIMNFLGAMTFTGVAKTITKDIVDPFALQNG
SLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAIAAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKV
VFKNFNLTKTNKNFRIFQIFTAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKWGTAKRMLITWVITLPISASL
AALFYSLLHLIF

Sequences:

>Translated_332_residues
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIVMAAIMNFLGAMTFTGVAKTITKDIVDPFALQNG
SLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAIAAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKV
VFKNFNLTKTNKNFRIFQIFTAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKWGTAKRMLITWVITLPISASL
AALFYSLLHLIF
>Mature_332_residues
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIVMAAIMNFLGAMTFTGVAKTITKDIVDPFALQNG
SLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAIAAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKV
VFKNFNLTKTNKNFRIFQIFTAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKWGTAKRMLITWVITLPISASL
AALFYSLLHLIF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=152, Percent_Identity=32.2368421052632, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI5803173, Length=158, Percent_Identity=28.4810126582279, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789360, Length=218, Percent_Identity=33.0275229357798, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789907, Length=218, Percent_Identity=32.5688073394495, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25146401, Length=154, Percent_Identity=33.7662337662338, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17539280, Length=165, Percent_Identity=29.6969696969697, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566716, Length=184, Percent_Identity=27.7173913043478, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=166, Percent_Identity=25.9036144578313, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=154, Percent_Identity=27.9220779220779, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35348; Mature: 35348

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIVMAAIMNFLGAMTF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVDPFALQNGSLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAI
HHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKVVFKNFNLTKTNKNFRIFQIF
HHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEHHHHH
TAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCCCCHHHH
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKW
HHCCCCEEEEEECCCHHHHHCCCEEEEEHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCC
GTAKRMLITWVITLPISASLAALFYSLLHLIF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIVMAAIMNFLGAMTF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVDPFALQNGSLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAI
HHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKVVFKNFNLTKTNKNFRIFQIF
HHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEHHHHH
TAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCCCCHHHH
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKW
HHCCCCEEEEEECCCHHHHHCCCEEEEEHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCC
GTAKRMLITWVITLPISASLAALFYSLLHLIF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]