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The map label for this gene is kinE [H]

Identifier: 49184273

GI number: 49184273

Start: 1297424

End: 1298632

Strand: Reverse

Name: kinE [H]

Synonym: BAS1256

Alternate gene names: 49184273

Gene position: 1298632-1297424 (Counterclockwise)

Preceding gene: 49184278

Following gene: 49184269

Centisome position: 24.84

GC content: 33.09

Gene sequence:

>1209_bases
ATGCTCGTATACCATTTATTTTGGAATCAAAAAGGGAAACGTTCTCCTAAATTAAATTCAGCAATATTTATTGTATTATG
TTGCCTCGTTACTATACTATGTATAACTTTCGCAGCAAAAACAATTTACGGATTTCAATTTGATATGCGTCATATCGTAT
TAATTGTCGGTACATTAACCGGAGGTCCTATTGCTGGTGGATCTATATTAGTTGTATTAAATATATACCGCTTCTTATTA
GGAGGAATAGGTGTTTTCCCATCACTTATCGGTTCTATCCTTCTATTTATTGTCCTATTATTTACATACAAATTTTTCAA
TCGAACTTCTAATCGTATAAAAATAACACTTGCTATTATCTACAGTCTCATATACGGATTTAGTTGGATACCTTTCTTCC
TTTCCAAGGTTACAAACAAGGCTGATTATATACCACATATTATTGTGTATGAGTTATGTACGATACTGGGTACGATTCTT
ATTTTATATTTACTACATATATTACAAACTCAAGTTCGACTCCAAAACGAACTTATGAATGCTGAAAAATTTCATTTAAT
TGGTGAAATGGCAGCGTCTATCTCTCATGAAATTCGCAATCCGTTAACTTCAACGAAAGGATTTTTGCAACTTTTACAAT
CAGATACATGCACTGAGCAGGAGCGAAAATTATATATCGACATAGCCATCAACGGAATCGAACAAGCAAACCACGTTCTT
ACAGATTATTTAACCTTTGCAAAACCAAGTATTGAAAAAGAACAAAGATTACAGTTAGAAGAAGAGCTGCTGCACGCATT
ATCGTTAATTACACCGCTTGCTAATTTAACAAATGTGCGTACACATTATATAAAGCAAAGCACTTCTTTTTTTATCGCGG
GAGAAAAACAAAAACTAAATCAATGCTTGTTAAATATTTTAAAAAACTGCATTGAGGCTATGCCAAAAGGCGGTGATCTT
TATTTCACATTAGTTCCTGATCATAAGCATATTCAGTTATATATAAAAGATACAGGAGTTGGTATGGATCAAGAACAAGT
AAAACGCCTTGGATCCCCTTTCTACTCAACAAAAGAAAAAGGTACTGGCCTTGGAATGATGGTCGTATTCAGTGTTGTTC
AAGCTATGAATGGAAAAATTGATATTATAAGCGAAAAAGGTACAGGTACAACCTTCTTATTAACTTTTCCACTCATACAA
AAAACGTGA

Upstream 100 bases:

>100_bases
TTTTATAGTATGATTAATTTAATGTAAGAAGAGAGGAGGCGTCCCACTGAAATCCTTTATTATTTATCTCTTTTTAAATA
TATTATTCATCCTTGTTACA

Downstream 100 bases:

>100_bases
TGAAATACTCATCACGTTTTTTCTGTTCCTTCAACTAATTCAGCAAAAGCTTTCACTTTGCCATGTGGTTTTTGATTTTC
TTTACGTGCTTTCCAAGCTC

Product: sporulation kinase B

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLTGGPIAGGSILVVLNIYRFLL
GGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAIIYSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTIL
ILYLLHILQTQVRLQNELMNAEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLNQCLLNILKNCIEAMPKGGDL
YFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEKGTGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQ
KT

Sequences:

>Translated_402_residues
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLTGGPIAGGSILVVLNIYRFLL
GGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAIIYSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTIL
ILYLLHILQTQVRLQNELMNAEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLNQCLLNILKNCIEAMPKGGDL
YFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEKGTGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQ
KT
>Mature_402_residues
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLTGGPIAGGSILVVLNIYRFLL
GGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAIIYSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTIL
ILYLLHILQTQVRLQNELMNAEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLNQCLLNILKNCIEAMPKGGDL
YFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEKGTGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQ
KT

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=254, Percent_Identity=28.740157480315, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788549, Length=227, Percent_Identity=29.5154185022026, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=28.82096069869, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=25.1063829787234, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786783, Length=219, Percent_Identity=26.027397260274, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994928, Length=221, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=292, Percent_Identity=23.2876712328767, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=28.2700421940928, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87081816, Length=220, Percent_Identity=26.8181818181818, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=22.4770642201835, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790300, Length=251, Percent_Identity=24.3027888446215, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45353; Mature: 45353

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLT
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEECC
GGPIAGGSILVVLNIYRFLLGGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
YSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTILILYLLHILQTQVRLQNELMN
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHH
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
QCLLNILKNCIEAMPKGGDLYFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEK
HHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCCCC
GTGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQKT
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHCC
>Mature Secondary Structure
MLVYHLFWNQKGKRSPKLNSAIFIVLCCLVTILCITFAAKTIYGFQFDMRHIVLIVGTLT
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEECC
GGPIAGGSILVVLNIYRFLLGGIGVFPSLIGSILLFIVLLFTYKFFNRTSNRIKITLAII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
YSLIYGFSWIPFFLSKVTNKADYIPHIIVYELCTILGTILILYLLHILQTQVRLQNELMN
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEKFHLIGEMAASISHEIRNPLTSTKGFLQLLQSDTCTEQERKLYIDIAINGIEQANHVL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHH
TDYLTFAKPSIEKEQRLQLEEELLHALSLITPLANLTNVRTHYIKQSTSFFIAGEKQKLN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
QCLLNILKNCIEAMPKGGDLYFTLVPDHKHIQLYIKDTGVGMDQEQVKRLGSPFYSTKEK
HHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCCCC
GTGLGMMVVFSVVQAMNGKIDIISEKGTGTTFLLTFPLIQKT
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]