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The map label for this gene is glcD [H]

Identifier: 49184228

GI number: 49184228

Start: 1256168

End: 1257580

Strand: Reverse

Name: glcD [H]

Synonym: BAS1210

Alternate gene names: 49184228

Gene position: 1257580-1256168 (Counterclockwise)

Preceding gene: 49184229

Following gene: 49184227

Centisome position: 24.05

GC content: 39.56

Gene sequence:

>1413_bases
ATGTTAGAAAAACAAATTATTGATTCATTCGTATCCATTGTTGGCGAAGATAATGTAGATACATCCAATATGGGGCGTTT
AACGTATAGTTATGATGCCACTCCAAACTTCCAAGCAATGCCTGATGCAGTCATTGCTCCTCGTAATACAAATGAAATAG
CTGAAGTATTAAAAGTATGTAACACTCACAAAATCCCTGTATATGTTCGTGGTTCCGGGACGAACCTTTGCGCAGGAACA
TGTCCACTTGAAGGTGGTATCGTCCTTATCTTCCGCCATATGAATAACATTTTAGAAATTGATGAAGAAAATTTAACAAT
TACTGTACAAGCTGGTGTTATTACGCTTGATATTATTAAAGCGGTGGAAGAAAAAGGTTTATTTTATCCACCAGATCCAA
GCTCAATGAAAATTTCTACAATTGGCGGTAACATTAATGAAAACTCAGGTGGATTACGTGGATTAAAATATGGCGTAACA
CGTGATTATGTGATGGGGCTTGAACTTGTCTTACCAAATGGTGATATTATTCGTACTGGTGGTAAATTAGCAAAAGATGT
AGCTGGTTACGATTTAACTCGTTTATTTATCGGTTCTGAAGGGACTCTTGGCGTCGTAACAGAAGCAATATTAAAACTTG
TCCCTATGCCTGAAACGAAGAAAACGATGCTTGCACTATATGAAGATATTAACGAAGCTGCACGTGCTGTTTCTTCTATT
ATTGCAAATAAAATCATTCCAGCGACACTTGAGTTTTTAGACCAACCGACAATTGAAGTAGTAGAAGAGTTCGCACAAAT
CGGTTTACCAACTGATGTAAAAGCAATTTTATTAATTGAACAAGACGGTCCGCCTGAAGTTGTTAATCGAGATATTGAAA
AGATGGCTAACGTTTGTCGTTCCATGAATGCAGTTGATGTTCGCGTCGCAAAAGATGAAGCGGAAGCAGATGCGCTTCGA
ACAGCTCGCCGTAGTGCACTGTCAGCACTGGCAAGATTAAAGCCTACTACAATATTAGAAGATGCAACGGTACCACGCTC
ACAGATTGCTCCGATGGTTGAAGCTATTAATGCCATTGCCAAAAAATATAATATTCCTATTTGTACGTTTGGGCATGTTG
GCGATGGCAACCTACACCCAACTTGTATGACAGATGCTCGCAACGAAGAAGAAATGCACCGAGCTGAACAAGCTTTTGCT
GAAATATTTGCGAAAGCAATCGAACTTGGTGGCACAATCACTGGTGAACATGGTGTTGGTGCGATGAAAGCTCCGTATTT
AGAAATGAAGTTAGGTAAAGAAGGTATTACCGCTATGCAAGGAATTAAACACGCCTTCGATCCAAATAACATTATGAATC
CAGGAAAGATGTTCGCGAAGGACACTCGTAAAAGAGTGGTGGTTGAGCGATGA

Upstream 100 bases:

>100_bases
ATAGAAAGAATCCCCCGACTTTTTTATCATTATAATGAAAGCACTGTGACAACGTTTTTTATTATGACTCATTACGTTAA
CAGTTTGAGGGGGATTTCCA

Downstream 100 bases:

>100_bases
CAACATTAAATAAAGAAAACATTCAAAAAGAATTTAAAGAACGATTAAGTGAAGATGAACTACTAAACTGTATGCGATGC
GGTTTCTGTTTACCAACTTG

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCRSMNAVDVRVAKDEAEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHVGDGNLHPTCMTDARNEEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER

Sequences:

>Translated_470_residues
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCRSMNAVDVRVAKDEAEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHVGDGNLHPTCMTDARNEEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER
>Mature_470_residues
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCRSMNAVDVRVAKDEAEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHVGDGNLHPTCMTDARNEEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=467, Percent_Identity=30.8351177730193, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI37595754, Length=490, Percent_Identity=29.3877551020408, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI119964728, Length=432, Percent_Identity=28.0092592592593, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI4501993, Length=228, Percent_Identity=28.5087719298246, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789351, Length=430, Percent_Identity=37.906976744186, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1787977, Length=512, Percent_Identity=27.34375, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI48994907, Length=484, Percent_Identity=25.4132231404959, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI48994914, Length=177, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=467, Percent_Identity=32.762312633833, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI71992381, Length=467, Percent_Identity=32.3340471092077, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI71992392, Length=384, Percent_Identity=32.8125, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17534361, Length=465, Percent_Identity=28.8172043010753, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17556096, Length=219, Percent_Identity=29.2237442922374, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320027, Length=445, Percent_Identity=30.561797752809, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320764, Length=474, Percent_Identity=28.6919831223629, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320023, Length=453, Percent_Identity=28.476821192053, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI18921117, Length=425, Percent_Identity=29.1764705882353, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24639277, Length=425, Percent_Identity=29.1764705882353, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24639275, Length=425, Percent_Identity=29.1764705882353, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24653753, Length=255, Percent_Identity=27.4509803921569, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 51048; Mature: 51048

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVC
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHH
NTHKIPVYVRGSGTNLCAGTCPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIK
CCCCCEEEEECCCCCEEEECCCCCCCEEEEEEHHHHHEEECCCCEEEEEECCEEHHHHHH
AVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVMGLELVLPNGDIIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHEEEEEEECCCCEEECC
GKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCR
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
SMNAVDVRVAKDEAEADALRTARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIA
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
KKYNIPICTFGHVGDGNLHPTCMTDARNEEEMHRAEQAFAEIFAKAIELGGTITGEHGVG
HHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVC
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHH
NTHKIPVYVRGSGTNLCAGTCPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIK
CCCCCEEEEECCCCCEEEECCCCCCCEEEEEEHHHHHEEECCCCEEEEEECCEEHHHHHH
AVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVMGLELVLPNGDIIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHEEEEEEECCCCEEECC
GKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCR
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
SMNAVDVRVAKDEAEADALRTARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIA
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
KKYNIPICTFGHVGDGNLHPTCMTDARNEEEMHRAEQAFAEIFAKAIELGGTITGEHGVG
HHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]