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The map label for this gene is cwlH [H]

Identifier: 49183853

GI number: 49183853

Start: 880712

End: 881905

Strand: Reverse

Name: cwlH [H]

Synonym: BAS0829

Alternate gene names: 49183853

Gene position: 881905-880712 (Counterclockwise)

Preceding gene: 49183854

Following gene: 49183850

Centisome position: 16.87

GC content: 37.19

Gene sequence:

>1194_bases
ATGGATTTATGCTCCTTCAGCCACATTCGAAGGTACGAAAGCAACACTATTCGAAAAAAAGTTTCTATGGAAAATAGAAG
CGCCGCATTTTTCAAAACACCATCTCATTTACCTACAAATAACGTTTATGGTGTTACATTTAAAGAAATGATTGTTCCAA
AAGGGAATGAAAATATTCGTCCTGGCTACGCAATGGAACCGAAATACATTACAATTCATGAAACTGCTAATACAAGTAAA
GGTGCGAACGCCTTAAATCATGCGAAGTACTTAGATAATCAAGCCCGCGGAAATACTGATCGTTCAGCATCTTGGCATTT
TACAGTAGATGACAAAGAAATTTATCAACATTTACCATTAAATGAAGTTGGTTGGCATGCCGGAAATAAAACTGGAAACT
ATGAATCTATCGGAATTGAAATTGCTGTGAATAGTGATGGGAATTATACGAAAGCGGTAGAAAACGCAAAAAAGTTAGCT
GCTTATTTAATGAATGAATTAAACATATCTCTAGATCACGTGCAAAAGCATCAATTTTGGAGCGGAAAAAACTGCCCTGC
ATTTATGATTCAACGCGGACAATGGGATGCTTTCTTAAAAGGTACGAACGCTTACTATAATGAACATCATAAAGATGTTA
TTCCGCCGCCAGAAGTCCCTCATGAAAAAGACGACATTACAGGTGGATGGTATGAACAAGACATTCGTCAATTGGCTGCT
AGAAAAATCATGTTCGGTGACGGCAATGGTTCTTACTGGCCAAACCGTCTTGTAACAAGAGCTGAGTTTGCAAATTTAAT
GTCGAGAGCTTTAAATTTACCTGCTGGAAACGCTAAATTTACAGATTTAAACGAAGCACATCCATCTTTAGTAGATGGAA
TTAACCGCGCCGCTAGCGCAGGTATCATTAATGGTCGTGGGAATAATAAATTTGATCCTAATGCTACAATAACACGTGAT
GAAGCTGTTATTATGATTGACCGTGCACTAGAATATAACTGGATTTATAGAAAAGAAGTGAAATTACCATTCACTGACCA
ACACTTAGCATACGATAAAAAAGCATTACAAAACGTTTATGCATATGGAATTGTAAAAGGGAACGAACGAAATGAATTCG
TACCAAAAGGCACAGCAACACGCGCTGAATCCGCCGCATTTTTAAATCGAATGCTTAAAGTTATTGAAGCTTAA

Upstream 100 bases:

>100_bases
TCCAATCATTTAAAGAAGAAATGAAATTACAGCCACAAACAGTAGCTCCTACTGAACAAGCTGGGAATTGGTTTAAAATT
CAAATGAACGGAACTGAGAA

Downstream 100 bases:

>100_bases
ATAAAAAAACAAAAGGCATAAGAGATAATATTTCTCTTATGCCTTTTATAAAACATATTAGTTATTTTTAGTCATAAATC
TTAAGCGTATCCCTAATAAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MDLCSFSHIRRYESNTIRKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNENIRPGYAMEPKYITIHETANTSK
GANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENAKKLA
AYLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHHKDVIPPPEVPHEKDDITGGWYEQDIRQLAA
RKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPSLVDGINRAASAGIINGRGNNKFDPNATITRD
EAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGTATRAESAAFLNRMLKVIEA

Sequences:

>Translated_397_residues
MDLCSFSHIRRYESNTIRKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNENIRPGYAMEPKYITIHETANTSK
GANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENAKKLA
AYLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHHKDVIPPPEVPHEKDDITGGWYEQDIRQLAA
RKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPSLVDGINRAASAGIINGRGNNKFDPNATITRD
EAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGTATRAESAAFLNRMLKVIEA
>Mature_397_residues
MDLCSFSHIRRYESNTIRKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNENIRPGYAMEPKYITIHETANTSK
GANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENAKKLA
AYLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHHKDVIPPPEVPHEKDDITGGWYEQDIRQLAA
RKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPSLVDGINRAASAGIINGRGNNKFDPNATITRD
EAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGTATRAESAAFLNRMLKVIEA

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 44916; Mature: 44916

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLCSFSHIRRYESNTIRKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNENIR
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEHHHEECCCCCCCCC
PGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPL
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHCCC
NEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENAKKLAAYLMNELNISLDHVQKHQFW
CHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
SGKNCPAFMIQRGQWDAFLKGTNAYYNEHHKDVIPPPEVPHEKDDITGGWYEQDIRQLAA
CCCCCCEEEEECCCCCEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPSLVDGINRAASA
HEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVY
CEEECCCCCCCCCCCEEECCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
AYGIVKGNERNEFVPKGTATRAESAAFLNRMLKVIEA
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLCSFSHIRRYESNTIRKKVSMENRSAAFFKTPSHLPTNNVYGVTFKEMIVPKGNENIR
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEHHHEECCCCCCCCC
PGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPL
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHCCC
NEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENAKKLAAYLMNELNISLDHVQKHQFW
CHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
SGKNCPAFMIQRGQWDAFLKGTNAYYNEHHKDVIPPPEVPHEKDDITGGWYEQDIRQLAA
CCCCCCEEEEECCCCCEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPSLVDGINRAASA
HEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVY
CEEECCCCCCCCCCCEEECCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
AYGIVKGNERNEFVPKGTATRAESAAFLNRMLKVIEA
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]