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The map label for this gene is braB [H]

Identifier: 49183789

GI number: 49183789

Start: 819068

End: 820384

Strand: Reverse

Name: braB [H]

Synonym: BAS0765

Alternate gene names: 49183789

Gene position: 820384-819068 (Counterclockwise)

Preceding gene: 49183790

Following gene: 49183784

Centisome position: 15.69

GC content: 35.61

Gene sequence:

>1317_bases
ATGCGTACAACTTTAAAACCAGCGCAAATACTTTCGATTAGTTTATTACTATTCGCAGTTTTCTTCGGTGCTGGTAATAT
GATTTTCCCGCCCCTTCTCGGCCTTTCTTCCGGAGAAAACATGTGGATTTCCATTACAGGATTTATTATTACAGACGTCG
GTTTATCTTTACTAGCTATCGTCGCTGTTGCCCTTGCCGGTGGTAGTTTTAACACTTTAGCAAGCCGTGTTCACCCAAAA
TTCGCAGCAATATTCGCTATCATTATTTATCTTTCAATCGGCCCACTATTTGTTATTCCACGAACTGGATCTGTATCTTA
TGAAATTGGGATTGCACCACTTTTCCCAGACCAATGGTACTCTATGTTAATCTTTAGTGCGATTTTCTTTACAGTCGTCT
ACTTCTTATCATTAAATCCATCAAAATTAGTAGATCATATCGGAAAAATATTAACACCAATTTTACTTGGAATTATTGCG
ATTATGGCAACAAAAGCAATTCTTTCACCAGGAACGTTTGCAGAACCTGTTGGTGACTATAAAGAAATTCCGTTTTTCAA
AGGATTTCTTGAAGGATTTTTAACGTTAGATGCAATCGGTGCACTCGTACTATCAACGATTGTTGTAAATGCAATTCGCC
AAAATGGTATACCAGAGAAGAAATCAATTGCGAAATATACAATTATTTGCGGAAGCATTGCCGCTCTATTTTTAACAATT
GTGTACTTCTTACTCGGTTATATCGGGGCTTCAAACGGCAACTTAGGACAATTCGAGAACGGTGGTCAACTATTAGCAAC
TGTTATGTATCACTTCTTTGGGACAAGCGGTAATGTTTTATTAAGCATCGCCATTATCTTCGCTTGTTTAACGACAGCAA
TTGGTGTTGTAAGTGCATTTGCCAACTATTTTGCAACAGTATTAACAAATGTTTCTTACAAAAAGCTCGTACTATACGTT
TGTATTTTTAGCTTCATTATTTCAAACTTAGGATTAAGTTTACTAATTAAAATTACTTTGCCTGTATTAATTATTCTATA
TCCAATTACAATCATTTTAATTTTCGTATCATTTATCGATAAATATACGAAACGTAAACCTTCTGTCTACATCGGGGCAA
TGATCGCAGCATTCCTTATTAGTTGTATTCATGCACTTGATAATGTGGGAATGATACCAAACTTTATTGCTAATATTGTA
CACACCATTCCTTTTTATAATTTAGGAATTGGCTGGATTATCCCAGCCATCATCGGTGGTATAATTGGATACTTTATTCC
GCAAACAGAAGCTGAAGGCGAAGTTTCTACAAAATAA

Upstream 100 bases:

>100_bases
CCTATTGCTTTCTAGTCATCAGACCTTTAAAATAATAACAGTTACTCGACAAATTGTTTGAATTTTCGACAAAAACACTC
AGTAGGAGGATTTTCTTTAT

Downstream 100 bases:

>100_bases
AGAAAAGCAGCAAGCATGAGATACATGCTTGCTGCTTTTTATTTTCACATCGTATGCTTAAAAGTAAATAACTCTTCTTT
CGACTGCACAACAGATTGTT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWISITGFIITDVGLSLLAIVAVALAGGSFNTLASRVHPK
FAAIFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWYSMLIFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLGIIA
IMATKAILSPGTFAEPVGDYKEIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIPEKKSIAKYTIICGSIAALFLTI
VYFLLGYIGASNGNLGQFENGGQLLATVMYHFFGTSGNVLLSIAIIFACLTTAIGVVSAFANYFATVLTNVSYKKLVLYV
CIFSFIISNLGLSLLIKITLPVLIILYPITIILIFVSFIDKYTKRKPSVYIGAMIAAFLISCIHALDNVGMIPNFIANIV
HTIPFYNLGIGWIIPAIIGGIIGYFIPQTEAEGEVSTK

Sequences:

>Translated_438_residues
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWISITGFIITDVGLSLLAIVAVALAGGSFNTLASRVHPK
FAAIFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWYSMLIFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLGIIA
IMATKAILSPGTFAEPVGDYKEIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIPEKKSIAKYTIICGSIAALFLTI
VYFLLGYIGASNGNLGQFENGGQLLATVMYHFFGTSGNVLLSIAIIFACLTTAIGVVSAFANYFATVLTNVSYKKLVLYV
CIFSFIISNLGLSLLIKITLPVLIILYPITIILIFVSFIDKYTKRKPSVYIGAMIAAFLISCIHALDNVGMIPNFIANIV
HTIPFYNLGIGWIIPAIIGGIIGYFIPQTEAEGEVSTK
>Mature_438_residues
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWISITGFIITDVGLSLLAIVAVALAGGSFNTLASRVHPK
FAAIFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWYSMLIFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLGIIA
IMATKAILSPGTFAEPVGDYKEIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIPEKKSIAKYTIICGSIAALFLTI
VYFLLGYIGASNGNLGQFENGGQLLATVMYHFFGTSGNVLLSIAIIFACLTTAIGVVSAFANYFATVLTNVSYKKLVLYV
CIFSFIISNLGLSLLIKITLPVLIILYPITIILIFVSFIDKYTKRKPSVYIGAMIAAFLISCIHALDNVGMIPNFIANIV
HTIPFYNLGIGWIIPAIIGGIIGYFIPQTEAEGEVSTK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=420, Percent_Identity=35.4761904761905, Blast_Score=274, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47219; Mature: 47219

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWISITGFIITDVGLSLLAI
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
VAVALAGGSFNTLASRVHPKFAAIFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWY
HHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCHHHHH
SMLIFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLGIIAIMATKAILSPGTFAEPVGDY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
KEIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIPEKKSIAKYTIICGSIAALFLTI
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VYFLLGYIGASNGNLGQFENGGQLLATVMYHFFGTSGNVLLSIAIIFACLTTAIGVVSAF
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ANYFATVLTNVSYKKLVLYVCIFSFIISNLGLSLLIKITLPVLIILYPITIILIFVSFID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYTKRKPSVYIGAMIAAFLISCIHALDNVGMIPNFIANIVHTIPFYNLGIGWIIPAIIGG
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHCCHHHHHHHHHHH
IIGYFIPQTEAEGEVSTK
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWISITGFIITDVGLSLLAI
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
VAVALAGGSFNTLASRVHPKFAAIFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWY
HHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCHHHHH
SMLIFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLGIIAIMATKAILSPGTFAEPVGDY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
KEIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIPEKKSIAKYTIICGSIAALFLTI
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VYFLLGYIGASNGNLGQFENGGQLLATVMYHFFGTSGNVLLSIAIIFACLTTAIGVVSAF
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ANYFATVLTNVSYKKLVLYVCIFSFIISNLGLSLLIKITLPVLIILYPITIILIFVSFID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYTKRKPSVYIGAMIAAFLISCIHALDNVGMIPNFIANIVHTIPFYNLGIGWIIPAIIGG
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHCCHHHHHHHHHHH
IIGYFIPQTEAEGEVSTK
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]