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The map label for this gene is colA [H]

Identifier: 49183549

GI number: 49183549

Start: 558430

End: 561327

Strand: Direct

Name: colA [H]

Synonym: BAS0523

Alternate gene names: 49183549

Gene position: 558430-561327 (Clockwise)

Preceding gene: 49183547

Following gene: 49183550

Centisome position: 10.68

GC content: 34.47

Gene sequence:

>2898_bases
ATGAACAAGAAATCAAAGATCAATAAAGTGATGCTTAGCATTAGTACAATGGCTCTATCACTGGGGGCAATTCAAACTCA
TGTATCAGCAGAAGAAAAGGTGCCATATAATGTATTACATTCGAAACCAGTTGGAATTGAAAAACCAGTAGATGAGATTG
GACACGTTTCTAAAGCGGAGGAAACATTATCGTTTCAAGAACGGCTAAAAGTAGGAGACTTTTCACAGCGACCAGCATCT
ATTACGAACAAAGTGACAGTAAAGCAAGTTAAAGAAAGCTATTCAATGGCTGATTTAAACAAAATGAATAATCAAGAATT
AGTTGAAACGTTAGGCAGTATTAAGTGGCATCAAATTACAGACTTATTCCAGTTTAACGAAGATGCAAAAGCCTTTTATA
AAGATAAAGGGAAAATGCAAGTCGTTATAGATGAATTAGCTCATCGGGGTAGTACATTTACAAAGGATGATTCAAAAGGA
ATTCAAACGTTTACGGAAGTGTTACGTTCAGCTTTTTATCTGGCATTTTATAATAACGAGTTAAGTGAATTAAATGAAAG
AAGCTTCCAGGACAAATGTTTACCTGCTTTAAAAGCAATCGCAAAAAATCCAAACTTTAAGCTTGGTACAACTGAACAAG
ATACAGTCGTATCTGCATACGGTAAATTAATTAGTAATGCGTCAAGCGATGTTGAAACAGTTCAATACGCATCGAATATT
TTAAAGCAATACAATGATAATTTTACTACGTATGTAAATGATCGAATGAAGGGACAAGCAATATACGATATTATGCAAGG
GATTGACTATGATATACAGTCTTACTTAATTGAAGCTCGTAAAGAAGCAAATGAAACGATGTGGTACGGGAAAGTAGATG
GGTTTATTAATGAAATAAATCGTATCGCTCTTCTAAATGAAGTAACACAAGAAAATAAGTGGCTCGTTAATAATGGAATT
TATTTTGCAAGTCGTTTAGGGAAGTTTCACAGTAATCCAAATAAAGGTTTAGAAGTTGTTACACAAGCAATGCATATGTA
TCCGCGCTTAAGTGAGCCGTACTTTGTCGCAGTAGAACAAATTACAACAAATTATAATGGAAAAGATTATAGCGGGAATA
CAGTAGATTTAGAGAAAATACGTAAAGAAGGAAAAGAGCAGTACTTACCAAAAACGTATACATTTGATGATGGATCTATC
GTATTTAAAACAGGAGATAAAGTATCGGAAGAAAAAATTAAGAGACTATACTGGGCTGCAAAGGAAGTAAAAGCACAGTA
TCATCGTGTAATTGGGAATGACAAAGCGTTAGAGCCGGGCAATGCAGATGATATATTAACGATAGTAATTTATAACAGTC
CAGAAGAGTACCAGTTAAATAGACAACTGTATGGATACGAAACAAATAACGGTGGAATTTATATTGAAGAAACAGGAACA
TTCTTTACATATGAGCGCACGCCAGAACAAAGCATTTATAGTTTAGAAGAGTTATTCCGTCATGAGTTTACTCATTATCT
TCAAGGAAGATATGAAGTGCCAGGTTTATTCGGAAGAGGAGATATGTATCAAAATGAAAGGTTAACTTGGTTCCAAGAAG
GCAATGCAGAGTTTTTCGCAGGGTCTACTCGAACAAATAATGTAGTGCCGAGAAAGAGCATCATTAGTGGATTATCATCT
GATCCTGCAAGCCGTTATACAGCAGAGCGCACATTATTTGCTAAATATGGTTCTTGGGATTTCTATAATTACTCGTTCGC
ATTGCAATCTTACTTATATACCCATCAATTTGAAACGTTTGATAAAATTCAAGATTTAATTCGTGCAAATGACGTAAAAA
ATTATGATGCCTATCGTGAGAATTTAAGTAAAGACCTTAAACTAAACGAAGAGTATCAAGAGTATATGCAGCACCTAATC
GATAATCAAGATAAATATAATGTACCGGAAGTAGCAGATGATTATTTAGCTGAACATACCCCAAAATCATTAACGGCAGT
AGAGAAAGAAATTACTGAAACGTTGCCGATGAAAGATGCAAAAATGACAAAACATAGCTCCCAATTCTTTAATACATTTA
CATTAGAAGGTACGTATACAGGTAGTGTAACAAAAGGTGATTCAGAAGATTGGAACGCAATGAGTAAGAAAGTAAATGAA
GCTTTGGAACAACTTGCGCAAAAAGAATGGAGTGGCTATAAAACTGTTACAGCATATTTTGTCAATTACGGTGTAAATAG
CTCAAATCAATTTGAATATGATGTAGTCTTCCACGGTATCGCAAAAGATGACGAAGAAAATAAAGCTCCAACGGTTAATA
TAAATGGCCCTTATAATGGACTTGTAAAAGAAGGTATTCAATTTAAAAGTGACGGCTCAAAAGATGAAGATGGAAAAATC
GTTTCTTATTTATGGGACTTTGGAGATGGAAGCACAAGTGCAGAAGTAAATCCGGTACATGTATATGAAAGCGAAGGTTC
ATATAAAGTAGCGTTAATAGTAAAAGATGATAAAGGAAAAGAGAGCAAAAGCGAAATAACGGTTACGGTTAAAGGTGGAA
GTTTAACAGAATCAGAACCAAATAATCGCCCAGAGGAAGCAAATCGTATTGGACTAAACACTACTATAAAAGGTAGTCTT
ATCGGCGGGGATCACACTGATGTTTATACATTTAATGTAGCATCAGCGAAAAATATTGATATTTCCGTTTTAAATGAATA
TGGAATCGGGATGACATGGGTACTTCACCATGAATCAGATATGCAAAATTATGCTGCTTACGGTCAAGCAAATGGAAATC
ATATAGAGGCAAACTTTAATGCAAAACCAGGTAAGTATTACTTGTATGTATATAAATATGATAATGGTGATGGAACATAC
GAATTATCAGTAAAATAA

Upstream 100 bases:

>100_bases
AAATGTAAGAAGAAATAATATGAAATATTGCATTTTATATTGTTGTATCCAGATTTTATGTTAAATTCGTGAATGGAATA
TTTGAATAGGGGGCAAGGAT

Downstream 100 bases:

>100_bases
GTACTTTTCCAATGTATGTTTAACTTTACTTTTTGTGGAAAATAGTATATTATATAGATATATAATATATCGGTTATTTT
CTAGGAGGAGCCTGTCACAA

Product: collagenase,putative

Products: NA

Alternate protein names: 120 kDa collagenase [H]

Number of amino acids: Translated: 965; Mature: 965

Protein sequence:

>965_residues
MNKKSKINKVMLSISTMALSLGAIQTHVSAEEKVPYNVLHSKPVGIEKPVDEIGHVSKAEETLSFQERLKVGDFSQRPAS
ITNKVTVKQVKESYSMADLNKMNNQELVETLGSIKWHQITDLFQFNEDAKAFYKDKGKMQVVIDELAHRGSTFTKDDSKG
IQTFTEVLRSAFYLAFYNNELSELNERSFQDKCLPALKAIAKNPNFKLGTTEQDTVVSAYGKLISNASSDVETVQYASNI
LKQYNDNFTTYVNDRMKGQAIYDIMQGIDYDIQSYLIEARKEANETMWYGKVDGFINEINRIALLNEVTQENKWLVNNGI
YFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSI
VFKTGDKVSEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDILTIVIYNSPEEYQLNRQLYGYETNNGGIYIEETGT
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSIISGLSS
DPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETFDKIQDLIRANDVKNYDAYRENLSKDLKLNEEYQEYMQHLI
DNQDKYNVPEVADDYLAEHTPKSLTAVEKEITETLPMKDAKMTKHSSQFFNTFTLEGTYTGSVTKGDSEDWNAMSKKVNE
ALEQLAQKEWSGYKTVTAYFVNYGVNSSNQFEYDVVFHGIAKDDEENKAPTVNINGPYNGLVKEGIQFKSDGSKDEDGKI
VSYLWDFGDGSTSAEVNPVHVYESEGSYKVALIVKDDKGKESKSEITVTVKGGSLTESEPNNRPEEANRIGLNTTIKGSL
IGGDHTDVYTFNVASAKNIDISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEANFNAKPGKYYLYVYKYDNGDGTY
ELSVK

Sequences:

>Translated_965_residues
MNKKSKINKVMLSISTMALSLGAIQTHVSAEEKVPYNVLHSKPVGIEKPVDEIGHVSKAEETLSFQERLKVGDFSQRPAS
ITNKVTVKQVKESYSMADLNKMNNQELVETLGSIKWHQITDLFQFNEDAKAFYKDKGKMQVVIDELAHRGSTFTKDDSKG
IQTFTEVLRSAFYLAFYNNELSELNERSFQDKCLPALKAIAKNPNFKLGTTEQDTVVSAYGKLISNASSDVETVQYASNI
LKQYNDNFTTYVNDRMKGQAIYDIMQGIDYDIQSYLIEARKEANETMWYGKVDGFINEINRIALLNEVTQENKWLVNNGI
YFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSI
VFKTGDKVSEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDILTIVIYNSPEEYQLNRQLYGYETNNGGIYIEETGT
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSIISGLSS
DPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETFDKIQDLIRANDVKNYDAYRENLSKDLKLNEEYQEYMQHLI
DNQDKYNVPEVADDYLAEHTPKSLTAVEKEITETLPMKDAKMTKHSSQFFNTFTLEGTYTGSVTKGDSEDWNAMSKKVNE
ALEQLAQKEWSGYKTVTAYFVNYGVNSSNQFEYDVVFHGIAKDDEENKAPTVNINGPYNGLVKEGIQFKSDGSKDEDGKI
VSYLWDFGDGSTSAEVNPVHVYESEGSYKVALIVKDDKGKESKSEITVTVKGGSLTESEPNNRPEEANRIGLNTTIKGSL
IGGDHTDVYTFNVASAKNIDISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEANFNAKPGKYYLYVYKYDNGDGTY
ELSVK
>Mature_965_residues
MNKKSKINKVMLSISTMALSLGAIQTHVSAEEKVPYNVLHSKPVGIEKPVDEIGHVSKAEETLSFQERLKVGDFSQRPAS
ITNKVTVKQVKESYSMADLNKMNNQELVETLGSIKWHQITDLFQFNEDAKAFYKDKGKMQVVIDELAHRGSTFTKDDSKG
IQTFTEVLRSAFYLAFYNNELSELNERSFQDKCLPALKAIAKNPNFKLGTTEQDTVVSAYGKLISNASSDVETVQYASNI
LKQYNDNFTTYVNDRMKGQAIYDIMQGIDYDIQSYLIEARKEANETMWYGKVDGFINEINRIALLNEVTQENKWLVNNGI
YFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSI
VFKTGDKVSEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDILTIVIYNSPEEYQLNRQLYGYETNNGGIYIEETGT
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSIISGLSS
DPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETFDKIQDLIRANDVKNYDAYRENLSKDLKLNEEYQEYMQHLI
DNQDKYNVPEVADDYLAEHTPKSLTAVEKEITETLPMKDAKMTKHSSQFFNTFTLEGTYTGSVTKGDSEDWNAMSKKVNE
ALEQLAQKEWSGYKTVTAYFVNYGVNSSNQFEYDVVFHGIAKDDEENKAPTVNINGPYNGLVKEGIQFKSDGSKDEDGKI
VSYLWDFGDGSTSAEVNPVHVYESEGSYKVALIVKDDKGKESKSEITVTVKGGSLTESEPNNRPEEANRIGLNTTIKGSL
IGGDHTDVYTFNVASAKNIDISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEANFNAKPGKYYLYVYKYDNGDGTY
ELSVK

Specific function: Unknown

COG id: COG3291

COG function: function code R; FOG: PKD repeat

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PKD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013510
- InterPro:   IPR007280
- InterPro:   IPR002169
- InterPro:   IPR013661
- InterPro:   IPR022409
- InterPro:   IPR000601 [H]

Pfam domain/function: PF01752 Peptidase_M9; PF08453 Peptidase_M9_N; PF00801 PKD; PF04151 PPC [H]

EC number: =3.4.24.3 [H]

Molecular weight: Translated: 109913; Mature: 109913

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50093 PKD ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSKINKVMLSISTMALSLGAIQTHVSAEEKVPYNVLHSKPVGIEKPVDEIGHVSKAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHCHHHHH
ETLSFQERLKVGDFSQRPASITNKVTVKQVKESYSMADLNKMNNQELVETLGSIKWHQIT
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
DLFQFNEDAKAFYKDKGKMQVVIDELAHRGSTFTKDDSKGIQTFTEVLRSAFYLAFYNNE
HHHHCCCHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCH
LSELNERSFQDKCLPALKAIAKNPNFKLGTTEQDTVVSAYGKLISNASSDVETVQYASNI
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKQYNDNFTTYVNDRMKGQAIYDIMQGIDYDIQSYLIEARKEANETMWYGKVDGFINEIN
HHHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
RIALLNEVTQENKWLVNNGIYFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQ
HHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEE
ITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSIVFKTGDKVSEEKIKRLYWAA
HHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHH
KEVKAQYHRVIGNDKALEPGNADDILTIVIYNSPEEYQLNRQLYGYETNNGGIYIEETGT
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHEECCEEEEEEECCCCEEEEECCC
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFA
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
GSTRTNNVVPRKSIISGLSSDPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETF
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHHHH
DKIQDLIRANDVKNYDAYRENLSKDLKLNEEYQEYMQHLIDNQDKYNVPEVADDYLAEHT
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
PKSLTAVEKEITETLPMKDAKMTKHSSQFFNTFTLEGTYTGSVTKGDSEDWNAMSKKVNE
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
ALEQLAQKEWSGYKTVTAYFVNYGVNSSNQFEYDVVFHGIAKDDEENKAPTVNINGPYNG
HHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCH
LVKEGIQFKSDGSKDEDGKIVSYLWDFGDGSTSAEVNPVHVYESEGSYKVALIVKDDKGK
HHHCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCC
ESKSEITVTVKGGSLTESEPNNRPEEANRIGLNTTIKGSLIGGDHTDVYTFNVASAKNID
CCCCEEEEEEECCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCE
ISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEANFNAKPGKYYLYVYKYDNGDGTY
EEEEECCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEEECCCCEE
ELSVK
EEEEC
>Mature Secondary Structure
MNKKSKINKVMLSISTMALSLGAIQTHVSAEEKVPYNVLHSKPVGIEKPVDEIGHVSKAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHCHHHHH
ETLSFQERLKVGDFSQRPASITNKVTVKQVKESYSMADLNKMNNQELVETLGSIKWHQIT
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
DLFQFNEDAKAFYKDKGKMQVVIDELAHRGSTFTKDDSKGIQTFTEVLRSAFYLAFYNNE
HHHHCCCHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCH
LSELNERSFQDKCLPALKAIAKNPNFKLGTTEQDTVVSAYGKLISNASSDVETVQYASNI
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKQYNDNFTTYVNDRMKGQAIYDIMQGIDYDIQSYLIEARKEANETMWYGKVDGFINEIN
HHHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
RIALLNEVTQENKWLVNNGIYFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQ
HHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEE
ITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSIVFKTGDKVSEEKIKRLYWAA
HHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHH
KEVKAQYHRVIGNDKALEPGNADDILTIVIYNSPEEYQLNRQLYGYETNNGGIYIEETGT
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHEECCEEEEEEECCCCEEEEECCC
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFA
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
GSTRTNNVVPRKSIISGLSSDPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETF
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHHHH
DKIQDLIRANDVKNYDAYRENLSKDLKLNEEYQEYMQHLIDNQDKYNVPEVADDYLAEHT
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
PKSLTAVEKEITETLPMKDAKMTKHSSQFFNTFTLEGTYTGSVTKGDSEDWNAMSKKVNE
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
ALEQLAQKEWSGYKTVTAYFVNYGVNSSNQFEYDVVFHGIAKDDEENKAPTVNINGPYNG
HHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCH
LVKEGIQFKSDGSKDEDGKIVSYLWDFGDGSTSAEVNPVHVYESEGSYKVALIVKDDKGK
HHHCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCC
ESKSEITVTVKGGSLTESEPNNRPEEANRIGLNTTIKGSLIGGDHTDVYTFNVASAKNID
CCCCEEEEEEECCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCE
ISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEANFNAKPGKYYLYVYKYDNGDGTY
EEEEECCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEEECCCCEE
ELSVK
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8282691; 11792842 [H]