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The map label for this gene is rocR [H]

Identifier: 49183492

GI number: 49183492

Start: 485870

End: 487273

Strand: Reverse

Name: rocR [H]

Synonym: BAS0464

Alternate gene names: 49183492

Gene position: 487273-485870 (Counterclockwise)

Preceding gene: 49183496

Following gene: 49183491

Centisome position: 9.32

GC content: 33.83

Gene sequence:

>1404_bases
ATGCGTACAGAAGAAATTAATTTAAAACAGATCATTGAAATGAATATGCTATATGAAACTTTACTCAATGAACTCGATAT
CGGGATTCATATTATTAATGAGGAAAGTAAAACGATTATCTATAACCGCAAAATGATGGAAATTGAATCAATGGAACGCT
CAGATGTGTTATATAAAAGTCCTTTAGAAGTATTCGCATTTGAAGAAAATAAAAATAGTACTCTTATAGAAGCATTAAAA
TTAGGAAAAACAAACAAAAATATAAAACAGACGTACTTTAACAATAAAGGGCAAGAAATTACGACGATTAACGATACTTT
CCCTATAATAGAAAATGGAAAAATTAAAGGCGCTATTGAAATTTCAAAAGAGATGAATCACTTAAAGCAAACAATAAAAA
TGGACTCTTTCCGAAAACAAAACACTAAATTTACCTTTGATCACATAATTGGTGATTCTGAAGCCATTCAATCAACCATT
GCGGAAGGAAAAAGGGTAATTCGTACATCCTCCTCCATACTTCTCGTAGGAGAAACAGGAACTGGAAAAGAACTATTTGC
ACAAAGTATCCATAATGAAAGTCAACGTTCAACAAAACCGTTCATTTCACAAAACTGTGCCGCTATACCTGATACGTTAA
TGGAAAGTTTATTATTTGGTACGAACCGGGGGGCATTTACAGGAGCAATCGATAAAGCTGGTTTATTTGAAGAAGCAAAC
GGAGGAACTTTGTTATTAGATGAGATCAACTCATTAAGTCCAGCACTTCAAGCGAAGTTACTGCGAGCTATACAAGAAAA
AACAATACGAAGAATCGGAGGCACACAAGAAAAAGAAATTGATGTTCGTATTATAGCAACTATTAATGAAGACCCTCTTG
AAGCTATTACACACAATCGATTACGAGAAGACTTATATTATCGATTAAGCGTCGTTACTTTATGTCTCCCGCCTTTACGT
GAACGAAAAGAGGATATTCCGGCTCTTGTTCAGCACTTTATCGAAAAGTACAACATTCAATTTGGACTTAGTGTAACAGA
TGTAGATATACATGTAAGAGAATTCTTGTATGCATATGATTGGCCTGGAAACGTCCGAGAATTGGAACATATCATTGAAG
GTTCAATGAACTTGGTTGAAGATGAAAATATTATTACGGCGTTTCATCTTCCTACTCGCTTTCGCGAACAAATAAAAAAA
GAATTCAATACGCAACCTTTCCTAACTAATAACGCCACTGATGCGCCAAAAACATTAAAGCACACAATAGCAGAAATGGA
AAAAAACTACATCACTCAAATTTTGAAAGAGTATCACGGTAATATTTCACAAGCTGCAAAGTTTTTGGGATTAAGTAGGC
AAAACTTACAATATCGAATTAAAAAACTGCATTTACACATATGA

Upstream 100 bases:

>100_bases
TTAAAAGGAAAATAGCAAAAAAATTTTGCGATTTTATAAGAATTTTAATAAAATTATAAAAAGTCAGTCTTCATAGGGTG
AAATGAGGAGAAGATAATCT

Downstream 100 bases:

>100_bases
AATGAACATTTCTCCAAGTGCAAAAATAACTTGGTAAAATATGTTACAATAAACATATAACATACTAGAAAGGAACGATA
TATATGAGTAATGATAACAA

Product: arginine utilization regulatory protein RocR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
LGKTNKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIEISKEMNHLKQTIKMDSFRKQNTKFTFDHIIGDSEAIQSTI
AEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAITHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNIQFGLSVTDVDIHVREFLYAYDWPGNVRELEHIIEGSMNLVEDENIITAFHLPTRFREQIKK
EFNTQPFLTNNATDAPKTLKHTIAEMEKNYITQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI

Sequences:

>Translated_467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
LGKTNKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIEISKEMNHLKQTIKMDSFRKQNTKFTFDHIIGDSEAIQSTI
AEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAITHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNIQFGLSVTDVDIHVREFLYAYDWPGNVRELEHIIEGSMNLVEDENIITAFHLPTRFREQIKK
EFNTQPFLTNNATDAPKTLKHTIAEMEKNYITQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
>Mature_467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
LGKTNKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIEISKEMNHLKQTIKMDSFRKQNTKFTFDHIIGDSEAIQSTI
AEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAITHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNIQFGLSVTDVDIHVREFLYAYDWPGNVRELEHIIEGSMNLVEDENIITAFHLPTRFREQIKK
EFNTQPFLTNNATDAPKTLKHTIAEMEKNYITQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=345, Percent_Identity=40.5797101449275, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1790437, Length=391, Percent_Identity=36.3171355498721, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI87082117, Length=358, Percent_Identity=36.5921787709497, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1789233, Length=479, Percent_Identity=31.3152400835073, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789087, Length=327, Percent_Identity=39.7553516819572, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1788905, Length=317, Percent_Identity=38.4858044164038, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87082152, Length=331, Percent_Identity=39.2749244712991, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1790299, Length=330, Percent_Identity=36.3636363636364, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1786524, Length=337, Percent_Identity=35.0148367952522, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787583, Length=343, Percent_Identity=32.6530612244898, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87081858, Length=445, Percent_Identity=28.5393258426966, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=35.1464435146443, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53556; Mature: 53556

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCCCHHCC
PLEVFAFEENKNSTLIEALKLGKTNKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIE
CCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCEECCCEEEEEE
ISKEMNHLKQTIKMDSFRKQNTKFTFDHIIGDSEAIQSTIAEGKRVIRTSSSILLVGETG
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHCHHEEEECCCEEEEECCC
TGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
CCHHHHHHHHHCHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAITHNR
CCEEEEECHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHH
LREDLYYRLSVVTLCLPPLRERKEDIPALVQHFIEKYNIQFGLSVTDVDIHVREFLYAYD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEECCEEEEHHHHHHHHHHHCC
WPGNVRELEHIIEGSMNLVEDENIITAFHLPTRFREQIKKEFNTQPFLTNNATDAPKTLK
CCCCHHHHHHHHHCCCCEECCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
HTIAEMEKNYITQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCCCHHCC
PLEVFAFEENKNSTLIEALKLGKTNKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIE
CCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCEECCCEEEEEE
ISKEMNHLKQTIKMDSFRKQNTKFTFDHIIGDSEAIQSTIAEGKRVIRTSSSILLVGETG
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHCHHEEEECCCEEEEECCC
TGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
CCHHHHHHHHHCHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAITHNR
CCEEEEECHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHH
LREDLYYRLSVVTLCLPPLRERKEDIPALVQHFIEKYNIQFGLSVTDVDIHVREFLYAYD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEECCEEEEHHHHHHHHHHHCC
WPGNVRELEHIIEGSMNLVEDENIITAFHLPTRFREQIKKEFNTQPFLTNNATDAPKTLK
CCCCHHHHHHHHHCCCCEECCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
HTIAEMEKNYITQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]