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The map label for this gene is truA

Identifier: 49183490

GI number: 49183490

Start: 484795

End: 485532

Strand: Direct

Name: truA

Synonym: BAS0462

Alternate gene names: 49183490

Gene position: 484795-485532 (Clockwise)

Preceding gene: 49183488

Following gene: 49183493

Centisome position: 9.27

GC content: 34.69

Gene sequence:

>738_bases
ATGAACAATTATAAATTAACAATTCAGTACGATGGTGCACGTTTTAAAGGGTGGCAACGTCTCGGGAATAACGATAATAC
GATTCAAGGGAAAATCGAAAGTGTCATATCTGAAATGGTCGGAAAAGAAACTGAAATTATCGGTTGCAGTAGAACAGACG
CTGGTGTACATGCTTTGAATCAAGTGGCTAACTTTCAAAGTGATGAGAAGTTAGTAGAACATAAAGTGAAAAAATATTTG
AATCAATATTTGCCAAATGATATTAGCATTACGAATGTAGAAGAAGTACATGATCGCTTCCATGCTCGTTACAATTCTAA
AGCAAAAACATATCTGTATAAAATTTGGAATGAAGAGCATACGAATCCATTTATGCGTAAATACAGCATGCATGTGAATA
AAAAATTAAATGTGAAAAGCATGAAAGAAGCTGCGAAACATTTAGTTGGTTCACATGACTTTACTGCTTTTTCAAATGCA
AAATCAAAGAAAAAGTCTATGGTACGAGAAGTATATTCACTTGAAGTAATGGAAGAGGCAGGATTTGTACAAATTAGAGT
AAGTGGCAACGGATTTCTTCATAACATGGTACGAAAAATTGTTGGAGCATTAATTGAAGTTGGATTAGGACAATTAGCTG
CAGAGGCGATTCCACAAATTCTAGAGGCGAAACAACGTAATCAAATTAATTGCCTTGCTGAGGCGAGTGGTTTGTATTTG
GAGAATGTTGAGTTTTAA

Upstream 100 bases:

>100_bases
ATACAGCTCATGTAGAAAATGTAGTGAAGCTTGTTAGAAAGTAAAGTGTATTCATATTCCCTCATGATATGAGGGAATAT
TTTCTGTGAAGGAGAATAAG

Downstream 100 bases:

>100_bases
TAATCAGTAAAAAATCGAGCTGTGTAGGCTCGATTTTTTATGTTTTGGCGTTAAGAAGATTTCTCTCTCTTCATACGTAT
CTTCAAATTCCAAAAAGCAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 2; tRNA-uridine isomerase I 2

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKETEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNA
KSKKKSMVREVYSLEVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEASGLYL
ENVEF

Sequences:

>Translated_245_residues
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKETEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNA
KSKKKSMVREVYSLEVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEASGLYL
ENVEF
>Mature_245_residues
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKETEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNA
KSKKKSMVREVYSLEVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEASGLYL
ENVEF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=280, Percent_Identity=28.9285714285714, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=34.1666666666667, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71985046, Length=266, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA2_BACAN (Q6I3T8)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   YP_026742.1
- ProteinModelPortal:   Q6I3T8
- SMR:   Q6I3T8
- EnsemblBacteria:   EBBACT00000013645
- EnsemblBacteria:   EBBACT00000021480
- EnsemblBacteria:   EBBACT00000082832
- GeneID:   2848361
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   bar:GBAA_0487
- TIGR:   BA_0487
- TIGR:   GBAA_0487
- GeneTree:   EBGT00050000002107
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- ProtClustDB:   PRK12434
- BioCyc:   BANT260799:BAS0462-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27928; Mature: 27928

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKETEIIGCSRTDAGVHALN
CCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
QVANFQSDEKLVEHKVKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEH
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCC
TNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNAKSKKKSMVREVYSLEVMEEA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEASGLYL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCEE
ENVEF
ECCCC
>Mature Secondary Structure
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKETEIIGCSRTDAGVHALN
CCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
QVANFQSDEKLVEHKVKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEH
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCC
TNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNAKSKKKSMVREVYSLEVMEEA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEASGLYL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCEE
ENVEF
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629