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The map label for this gene is yfkH [H]

Identifier: 49183423

GI number: 49183423

Start: 427849

End: 428718

Strand: Direct

Name: yfkH [H]

Synonym: BAS0395

Alternate gene names: 49183423

Gene position: 427849-428718 (Clockwise)

Preceding gene: 49183422

Following gene: 49183426

Centisome position: 8.18

GC content: 36.09

Gene sequence:

>870_bases
ATGAGAAAAATTTTAGAAAAGGTAAGAAGAAATCGTACGTATTCGTTTGGTAAAGATTTGTATGACCGGACGATGCGCGA
TGATGTAGCGGGCTTGGCAGCACAGCTCGCTTATTTCTTCTTGCTTGCGATTTTTCCTGGGCTTGTTTTCTTAATTACGC
TTCTTGGATTCATTGACCTTCAAACAGAAAGTGTGCTCAATTTATTAGAACCGTACGTACCCGAAGATGCAATGTCCTTA
ATTGAAGTAAACGTGGATAAAGTTGTAAATGAGCAAAATGGTGGTTTATTATCATTTGGTTTATTGTCAATGTTATGGTT
TGCTTCAAATGGGGTAAATGCGGTTATGAATGCTTTTAACCGTGCGTATGATGTAACAGAAACGCGTTCTTTTATTAAAA
CGAGAGCTTTATCAATTGTATTTACATTAGCGATTATTTTTATGATTGTGTTTGCGCTAATTGTTCCAGTGTTTGGACAA
GTCATTGGTGCAGCGGTATTTAAAGCAATTGGTTTATCGGATAGTTTTTCATACGTGTGGAGCATTACACGGTTAGTAGC
GAGTTTCTTCGTACTATTTGCGTTGTTTAGTTTTTTATATACATTTGCACCAGATCGAAAGTTAAAAAGAAGAGAAGTCA
TTTCAGGAGCAACATTTGCTACTGTAGGATGGATTGTGGTATCGTACTCATTTGCTTATTATGTAGATAAGTTTGCAAAT
TACGCGAATACATATGGTGGTCTCGGTGGTATCATTATTTTAATGTTATGGTTTTATTTAACTGGATGGGTAATTTTACT
TGGCGGCGAAATTAATGGTTTACTCCATCATTATAGGACGGGTGACAATAATTCCCGTAATGAAAAATGA

Upstream 100 bases:

>100_bases
GTGAACTATTTATGAAAGACTCTAATGTTGAGGAAAAAAAACTGAAGCGTACATTGAGTTGAAGCAAGATAAAGAAGAGA
AGAAAAGCGTTTAAACATAT

Downstream 100 bases:

>100_bases
CTTCTTGTTCCATAATAATAAGGAACAGGGGGGATATTTCATGAGTAATAATAAAAAGAATCACAACAATAAAAATAACA
ATAAACAAAAGAGCAGTTCG

Product: ribonuclease BN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRKILEKVRRNRTYSFGKDLYDRTMRDDVAGLAAQLAYFFLLAIFPGLVFLITLLGFIDLQTESVLNLLEPYVPEDAMSL
IEVNVDKVVNEQNGGLLSFGLLSMLWFASNGVNAVMNAFNRAYDVTETRSFIKTRALSIVFTLAIIFMIVFALIVPVFGQ
VIGAAVFKAIGLSDSFSYVWSITRLVASFFVLFALFSFLYTFAPDRKLKRREVISGATFATVGWIVVSYSFAYYVDKFAN
YANTYGGLGGIIILMLWFYLTGWVILLGGEINGLLHHYRTGDNNSRNEK

Sequences:

>Translated_289_residues
MRKILEKVRRNRTYSFGKDLYDRTMRDDVAGLAAQLAYFFLLAIFPGLVFLITLLGFIDLQTESVLNLLEPYVPEDAMSL
IEVNVDKVVNEQNGGLLSFGLLSMLWFASNGVNAVMNAFNRAYDVTETRSFIKTRALSIVFTLAIIFMIVFALIVPVFGQ
VIGAAVFKAIGLSDSFSYVWSITRLVASFFVLFALFSFLYTFAPDRKLKRREVISGATFATVGWIVVSYSFAYYVDKFAN
YANTYGGLGGIIILMLWFYLTGWVILLGGEINGLLHHYRTGDNNSRNEK
>Mature_289_residues
MRKILEKVRRNRTYSFGKDLYDRTMRDDVAGLAAQLAYFFLLAIFPGLVFLITLLGFIDLQTESVLNLLEPYVPEDAMSL
IEVNVDKVVNEQNGGLLSFGLLSMLWFASNGVNAVMNAFNRAYDVTETRSFIKTRALSIVFTLAIIFMIVFALIVPVFGQ
VIGAAVFKAIGLSDSFSYVWSITRLVASFFVLFALFSFLYTFAPDRKLKRREVISGATFATVGWIVVSYSFAYYVDKFAN
YANTYGGLGGIIILMLWFYLTGWVILLGGEINGLLHHYRTGDNNSRNEK

Specific function: 3' To 5' Exoribonuclease Necessary For 3'Maturation Of tRNA Molecules. [C]

COG id: COG1295

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790319, Length=308, Percent_Identity=23.7012987012987, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017039
- InterPro:   IPR004664 [H]

Pfam domain/function: PF03631 Ribonuclease_BN [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 32544; Mature: 32544

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]