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The map label for this gene is topB

Identifier: 49183389

GI number: 49183389

Start: 391511

End: 393700

Strand: Reverse

Name: topB

Synonym: BAS0361

Alternate gene names: 49183389

Gene position: 393700-391511 (Counterclockwise)

Preceding gene: 49183392

Following gene: 49183383

Centisome position: 7.53

GC content: 36.58

Gene sequence:

>2190_bases
ATGGCAAAAAGCGTTGTAATCGCTGAAAAACCTTCCGTTGCACGAGATATTGCGCGTGTACTGAAGTGCGACAAAAAAGG
AAACGGCTACCTTGAAGGTAGTAAATATATTGTAACTTGGGCTTTAGGTCATCTTGTTACATTAGCTGATCCAGAAAGCT
ATGATGTGAAATATAAAAAGTGGAATTTAGAAGATTTACCGATGCTACCTGAGCGTTTGAAATTAACTGTTATTAAACAA
ACAGGGAAACAATTTAATGCTGTGAAGAGTCAGCTTCTTAGAAAAGATGTAAATGAAATCATTGTAGCGACAGACGCTGG
ACGTGAAGGAGAATTGGTTGCTCGTTGGATTATTGATAAGGTTCGAATTAACAAACCAATTAAACGTCTATGGATTTCAT
CTGTTACTGATAAAGCAATTAAAGATGGTTTCGCAAATTTAAAACCAGGTAAAGCATATGACAATTTATACGCTTCAGCT
GTCGCACGTTCAGAAGCTGACTGGTATATCGGTCTTAACGCGACTCGAGCTTTAACGACTCGCTTTAATGCCCAGCTTAA
CTGTGGCCGTGTGCAAACACCTACTGTTGCTATGATCGCTAATCGTGAAGATGAAATAAAGAACTTCAAAGCTCAAACTT
ACTACGGCATTGAAGCTCAAACAACAAATCAATTAAAACTAACGTGGCAAGATGCAAATGGCAATAGCCGCAGTTTTAAT
AAAGAAAAAATTGATGGTATTGTAAAAGGTTTAGATAAACATAATGCTACTGTTTTAGAAATTGATAAAAAACAGAAGAA
GTCATTCTCTCCTGGTCTTTACGATTTAACTGAATTGCAACGTGATGCCAATAAAAAGTTCGGTTACTCTGCGAAAGAAA
CATTGAATATTATGCAGAAATTGTATGAACAACATAAAGTGCTAACATACCCTCGTACAGATTCGCGTTACATTTCATCT
GATATCGTTGGAACACTTCCAGAACGTCTAAAGGCGTGCGGCGTTGGGGAATATCGTCCTTTAGCACATAAAGTATTACA
AAAGCCTATCAAGGCTAATAAATCATTTGTTGATGATAGTAAAGTAAGCGATCACCACGCAATTATTCCGACAGAAGGAT
ACGTTAACTTCTCAGCCTTCACAGATAAAGAACGTAAAATTTATGATTTAGTTGTCAAACGTTTCTTAGCTGTTTTATTC
CCAGCATTCGAATACGAACAACTAACGTTACGCACAAAGGTCGGCAATGAAACATTCATTGCACGCGGAAAGACAATTTT
ACATGCCGGTTGGAAAGAAGTATATGAAAATCGCTTTGAAGATGATGATGTAACTGATGACGTAAAAGAGCAACTTTTAC
CTCGCATTGAAAAAGGCGATACATTAACTGTAAAGTTAATTATGCAAACATCAGGTCAAACGAAAGCACCTGCACGTTTT
AACGAAGCGACTTTACTTTCAGCAATGGAAAATCCTACAAAATATATGGATACGCAAAATAAACAACTTGCTGATACGTT
AAAATCAACTGGTGGATTAGGTACTGTGGCAACACGAGCTGATATTATCGACAAACTATTCAATTCATTCTTAATTGAAA
AACGCGGGAAAGATATTCACATTACTTCAAAAGGCCGTCAGTTACTTGATTTAGTACCAGAAGAGTTAAAATCACCTACA
CTAACTGGTGAGTGGGAACAAAAACTAGAGGCAATTGCAAAAGGTAAACTGAAAAAAGAAGTATTCATTTCCGAAATGAA
GAACTATACGAAAGAAATTGTTTCTGAAATTAAATCGAGTGATAAAAAATATAAACATGACAACATTTCAACAAAGTCTT
GTCCAGATTGCGGGAAACCAATGCTAGAGGTAAACGGCAAAAAAGGAAAAATGCTCGTTTGCCAAGACCGTGAATGTGGT
CATCGTAAAAATGTATCTCGTACAACAAACGCTCGTTGCCCTCAGTGTAAGAAGAAGTTAGAATTACGTGGTGAAGGTGC
AGGACAAATCTTTGCATGTAAATGTGGCTATCGCGAGAAATTATCCACATTCCAAGAAAGACGTAAAAAGGAATCTGGAA
ACAAAGCTGATAAGCGTGACGTTCAAAAATATATGAAACAGCAGAAAAAAGAGGAAGAACCATTAAATAACCCATTCGCA
GAAGCATTAAAGAAATTAAAATTTGATTAA

Upstream 100 bases:

>100_bases
GTTCATTCCACTAAAAACCGTTCAAATATGGAGCGAAACTGAATCTCAAGAAGATGTATGCTATAGTTAACGTATGGAAT
TTTGAAAGGATGTTTTTAAT

Downstream 100 bases:

>100_bases
ACAAAAAGGTTCCTACCTCTATCGGTAGGAACCTTTTATGTTAGTGAATGATTTTACTTCCTTTTGCATTCTCAACGAAT
TTTTCACTTGATTTGTTAAT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MAKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKKWNLEDLPMLPERLKLTVIKQ
TGKQFNAVKSQLLRKDVNEIIVATDAGREGELVARWIIDKVRINKPIKRLWISSVTDKAIKDGFANLKPGKAYDNLYASA
VARSEADWYIGLNATRALTTRFNAQLNCGRVQTPTVAMIANREDEIKNFKAQTYYGIEAQTTNQLKLTWQDANGNSRSFN
KEKIDGIVKGLDKHNATVLEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISS
DIVGTLPERLKACGVGEYRPLAHKVLQKPIKANKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLF
PAFEYEQLTLRTKVGNETFIARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARF
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPT
LTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECG
HRKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFA
EALKKLKFD

Sequences:

>Translated_729_residues
MAKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKKWNLEDLPMLPERLKLTVIKQ
TGKQFNAVKSQLLRKDVNEIIVATDAGREGELVARWIIDKVRINKPIKRLWISSVTDKAIKDGFANLKPGKAYDNLYASA
VARSEADWYIGLNATRALTTRFNAQLNCGRVQTPTVAMIANREDEIKNFKAQTYYGIEAQTTNQLKLTWQDANGNSRSFN
KEKIDGIVKGLDKHNATVLEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISS
DIVGTLPERLKACGVGEYRPLAHKVLQKPIKANKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLF
PAFEYEQLTLRTKVGNETFIARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARF
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPT
LTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECG
HRKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFA
EALKKLKFD
>Mature_728_residues
AKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKKWNLEDLPMLPERLKLTVIKQT
GKQFNAVKSQLLRKDVNEIIVATDAGREGELVARWIIDKVRINKPIKRLWISSVTDKAIKDGFANLKPGKAYDNLYASAV
ARSEADWYIGLNATRALTTRFNAQLNCGRVQTPTVAMIANREDEIKNFKAQTYYGIEAQTTNQLKLTWQDANGNSRSFNK
EKIDGIVKGLDKHNATVLEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISSD
IVGTLPERLKACGVGEYRPLAHKVLQKPIKANKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFP
AFEYEQLTLRTKVGNETFIARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARFN
EATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPTL
TGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGH
RKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFAE
ALKKLKFD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI4507635, Length=622, Percent_Identity=25.8842443729904, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI10835218, Length=600, Percent_Identity=24.3333333333333, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1788061, Length=620, Percent_Identity=32.4193548387097, Blast_Score=336, Evalue=3e-93,
Organism=Escherichia coli, GI1787529, Length=669, Percent_Identity=26.0089686098655, Blast_Score=162, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI32563869, Length=747, Percent_Identity=25.3012048192771, Blast_Score=145, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=642, Percent_Identity=25.3894080996885, Blast_Score=115, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=698, Percent_Identity=21.6332378223496, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585251, Length=599, Percent_Identity=26.0434056761269, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640096, Length=618, Percent_Identity=24.4336569579288, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP3_BACAH (A0R979)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_893279.1
- ProteinModelPortal:   A0R979
- SMR:   A0R979
- STRING:   A0R979
- EnsemblBacteria:   EBBACT00000070117
- GeneID:   4546423
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_0373
- eggNOG:   COG0550
- GeneTree:   EBGT00070000031993
- HOGENOM:   HBG585507
- OMA:   CPECHKK
- ProtClustDB:   PRK07726
- BioCyc:   BTHU412694:BALH_0373-MONOMER
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01056

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 82813; Mature: 82682

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKK
CCCCEEEECCCHHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHEEEECCCCCCCCEEEE
WNLEDLPMLPERLKLTVIKQTGKQFNAVKSQLLRKDVNEIIVATDAGREGELVARWIIDK
CCCCCCCCCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHH
VRINKPIKRLWISSVTDKAIKDGFANLKPGKAYDNLYASAVARSEADWYIGLNATRALTT
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHH
RFNAQLNCGRVQTPTVAMIANREDEIKNFKAQTYYGIEAQTTNQLKLTWQDANGNSRSFN
HHCCEECCCCCCCCEEEEEECCHHHHHCCCCCEEECCEECCCCEEEEEEECCCCCCCCCC
KEKIDGIVKGLDKHNATVLEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQK
HHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LYEQHKVLTYPRTDSRYISSDIVGTLPERLKACGVGEYRPLAHKVLQKPIKANKSFVDDS
HHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
KVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFPAFEYEQLTLRTKVGNETFI
CCCCCCEEECCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
ARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARF
ECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCC
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIH
CHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ITSKGRQLLDLVPEELKSPTLTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSS
EECCCHHHHHHCHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
DKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGHRKNVSRTTNARCPQCKKKL
CCHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
ELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFA
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
EALKKLKFD
HHHHHHCCC
>Mature Secondary Structure 
AKSVVIAEKPSVARDIARVLKCDKKGNGYLEGSKYIVTWALGHLVTLADPESYDVKYKK
CCCEEEECCCHHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHEEEECCCCCCCCEEEE
WNLEDLPMLPERLKLTVIKQTGKQFNAVKSQLLRKDVNEIIVATDAGREGELVARWIIDK
CCCCCCCCCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHH
VRINKPIKRLWISSVTDKAIKDGFANLKPGKAYDNLYASAVARSEADWYIGLNATRALTT
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHH
RFNAQLNCGRVQTPTVAMIANREDEIKNFKAQTYYGIEAQTTNQLKLTWQDANGNSRSFN
HHCCEECCCCCCCCEEEEEECCHHHHHCCCCCEEECCEECCCCEEEEEEECCCCCCCCCC
KEKIDGIVKGLDKHNATVLEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQK
HHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LYEQHKVLTYPRTDSRYISSDIVGTLPERLKACGVGEYRPLAHKVLQKPIKANKSFVDDS
HHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
KVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFPAFEYEQLTLRTKVGNETFI
CCCCCCEEECCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
ARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARF
ECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCC
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIH
CHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ITSKGRQLLDLVPEELKSPTLTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSS
EECCCHHHHHHCHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
DKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGHRKNVSRTTNARCPQCKKKL
CCHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
ELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFA
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
EALKKLKFD
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA