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The map label for this gene is ykfB [H]

Identifier: 49183352

GI number: 49183352

Start: 348286

End: 349395

Strand: Reverse

Name: ykfB [H]

Synonym: BAS0324

Alternate gene names: 49183352

Gene position: 349395-348286 (Counterclockwise)

Preceding gene: 49183354

Following gene: 49183351

Centisome position: 6.68

GC content: 39.28

Gene sequence:

>1110_bases
ATGAAAATTACAGCTATTCACCTTTACGCAATTCGTTTACCGCTTCGCGATCCGTTTGTTATTAGTTATGGTTCTTATTC
TGATATGCCTTCTATTATCGTCAAAATGGAAACAGACGAAGGTATTATCGGTTACGGTGAAGGCGTTGCTGATGATCACG
TCACAGGTGAATCATGGGAAAGTACTTTCCATATTTTAAAGCATACGCTAGCACCTGCACTAATCGGACAAAATCCAATG
AATATAGAAAAAATACACGATATGATGGACAATACAATTTACGGTGTTCCAACAGCGAAAGCTGCGATTGATATTGCTTG
TTTTGATATAATGGGTAAAAAATTAAATCAACCTGTATATCAATTAATTGGTGGACGATACCATGAAGAATTTCCTATCA
CTCACGTATTAAGTATCGCAGATCCAGAAGAAATGGCCGAAGAAGCTGCCTCTATGATTCAGAAAGGTTACCAATCTTTC
AAAATGAAAGTCGGTACAAATGTAAAAGAAGATGTAAAACGAATTGAAGCAGTACGTGAGCGTGTAGGAAATGACATCGC
AATTCGTGTTGATGTAAACCAAGGTTGGAAAAATAGCGCCAACACATTAATGGCACTTCGTTCATTAGGACATTTAAACA
TCGACTGGATTGAACAGCCTGTCATCGCAGACGATATTGACGCAATGGCTCACATTCGTTCGAAAACAGACCTTCCGCTT
ATGATTGATGAAGGATTAAAGGGCTCTCGTGAAATGCGCCAAATTATTAATTTAGACGCGGCTGATAAAGTAAACATAAA
ACTAATGAAATGCGGCGGCATATACCCAGCTGTAAAACTTGCTCATCAAGCTGAAATGGCAGGCATTGAATGCCAAGTTG
GATCCATGGTCGAATCATCCGTTGCCTCTTCCGCGGGATTCCATGTCGCTTTTTCGAAAAAAATTATTACGAGCGTTGAA
CTTACAGGTCCATTAAAATTCACGAAGGACATCGGAAACTTACATTACGACGTACCATTTATTCGCTTAAACGAAAAGCC
AGGACTCGGCATTGAAATAAATGAAGATACACTACGAGAACTCACCGTCTTTCAAGACATTGTACGCTAA

Upstream 100 bases:

>100_bases
ATCATCATTTCTCTAACTCCTTATCATTAATTAACGACTATTTAACGTCAACTATATCAAATGTATAAAACTATTGTAAA
CAGGAAGGGGTCTCTACCTA

Downstream 100 bases:

>100_bases
AGGAGGGCTAACGCATGACAGTTCTATACGAGGGCAGATTAAAACAAAATAACGAGCGATTTCACGTTACATTACTATCA
CTAGAGTACATCGAACAAAT

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKITAIHLYAIRLPLRDPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWESTFHILKHTLAPALIGQNPM
NIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVYQLIGGRYHEEFPITHVLSIADPEEMAEEAASMIQKGYQSF
KMKVGTNVKEDVKRIEAVRERVGNDIAIRVDVNQGWKNSANTLMALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
MIDEGLKGSREMRQIINLDAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESSVASSAGFHVAFSKKIITSVE
LTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLRELTVFQDIVR

Sequences:

>Translated_369_residues
MKITAIHLYAIRLPLRDPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWESTFHILKHTLAPALIGQNPM
NIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVYQLIGGRYHEEFPITHVLSIADPEEMAEEAASMIQKGYQSF
KMKVGTNVKEDVKRIEAVRERVGNDIAIRVDVNQGWKNSANTLMALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
MIDEGLKGSREMRQIINLDAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESSVASSAGFHVAFSKKIITSVE
LTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLRELTVFQDIVR
>Mature_369_residues
MKITAIHLYAIRLPLRDPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWESTFHILKHTLAPALIGQNPM
NIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVYQLIGGRYHEEFPITHVLSIADPEEMAEEAASMIQKGYQSF
KMKVGTNVKEDVKRIEAVRERVGNDIAIRVDVNQGWKNSANTLMALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
MIDEGLKGSREMRQIINLDAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESSVASSAGFHVAFSKKIITSVE
LTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLRELTVFQDIVR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=389, Percent_Identity=26.4781491002571, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081876, Length=339, Percent_Identity=29.2035398230088, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI226510960, Length=346, Percent_Identity=23.121387283237, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 40962; Mature: 40962

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITAIHLYAIRLPLRDPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWE
CEEEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCHH
STFHILKHTLAPALIGQNPMNIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCHHH
QLIGGRYHEEFPITHVLSIADPEEMAEEAASMIQKGYQSFKMKVGTNVKEDVKRIEAVRE
HHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RVGNDIAIRVDVNQGWKNSANTLMALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
HCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCE
MIDEGLKGSREMRQIINLDAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESS
EECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHHHH
VASSAGFHVAFSKKIITSVELTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLRE
HHHCCCEEEEEHHHHHEEEEECCCCHHHHHHCCEEECCCEEEECCCCCCCEEECHHHHHH
LTVFQDIVR
HHHHHHHHC
>Mature Secondary Structure
MKITAIHLYAIRLPLRDPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWE
CEEEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCHH
STFHILKHTLAPALIGQNPMNIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCHHH
QLIGGRYHEEFPITHVLSIADPEEMAEEAASMIQKGYQSFKMKVGTNVKEDVKRIEAVRE
HHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RVGNDIAIRVDVNQGWKNSANTLMALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
HCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCE
MIDEGLKGSREMRQIINLDAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESS
EECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHHHH
VASSAGFHVAFSKKIITSVELTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLRE
HHHCCCEEEEEHHHHHEEEEECCCCHHHHHHCCEEECCCEEEECCCCCCCEEECHHHHHH
LTVFQDIVR
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]