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The map label for this gene is nupC [H]

Identifier: 49183345

GI number: 49183345

Start: 340869

End: 342065

Strand: Reverse

Name: nupC [H]

Synonym: BAS0317

Alternate gene names: 49183345

Gene position: 342065-340869 (Counterclockwise)

Preceding gene: 49183351

Following gene: 49183344

Centisome position: 6.54

GC content: 36.26

Gene sequence:

>1197_bases
ATGCAATATGTAATGAGCATTATCGGTATTCTTGTCGTTTTAGGTTTATGTTTTGCTTTGTCAAACAACAAAAGTAAAAT
CAACTTCCGTGCAATTGCAATTATGATTGGTTTCCAAATTTTAATCGGTTGGTTTATGTTTGGCACAAAAATTGGTCAAC
AAATTATCATCTTCATTAGTAAAGTTTTCAACAAACTAATTAAACTTGGTACGACAGGCGTCGATTTTCTCTTTAATGGA
ATTCAAAGAGATTTTGTCTTTTTCTTAAACGTATTATTAATTATCGTATTTTTCTCAGCACTACTTTCTATCTTTAGTTA
TTTAGGTGTTTTACCATTCATCGTTCGCGTTGTCGGCGGTGCCATTTCAAAAGTTACTGGTTTACCACGCGTTGAATCAT
TCCACGCAGTAAACTCTGTATTCTTCGGTTCAAGTGAAGCTTTAATCGTTATTAAAAATGATTTACAGCATTTTAACAAA
AACCGTATGTTTATCATTTGTTGTTCTGCGATGAGCTCAGTTTCTGCTTCTGTTACAGCATCATACGTAATGATGTTAGA
TGCAAAATATGTATTAGCAGCTCTTCCATTAAACTTATTCTCAAGCTTAATCGTTTGTTCGTTATTAACACCTGTTGATA
CGAAAAAGGAAGACGAAGTAGTTCAGAAATTTGACCGAACTGTATTCGGGGACAGCTTTATCGGTGCAATGATTAACGGT
GCGCTTGACGGTTTAAAAGTAGCAGGTATCGTTGCCGCATTAATGATCGCTTTCATCGGTGTGATGGAAGTTGTAAACTA
CGTAATTAGCGCAGCTTCAGGTGCAATGGGACATGCCGTTACGTTACAACAAATCTTTGGTTACGTACTTGCTCCATTTG
CATTCTTAATGGGTATTCCAGCTCAAGATATTATCCCAGCTGGCGGAATTATGGGTACGAAGATTGTATTAAACGAGTTT
GTAGCAATCCTTGATTTAAAAGGTGTTGCAGCAACATTATCTCCACGTACAGTTGGAATCGTTACAGTATTCTTAATTAG
CTTCGCAAGTATTAGCCAAATTGGAGCGATCGTTGGTACAATTCGTGCTCTTTCTGAGAAACAAGGAAGCATCGTATCGA
AATTTGGTTGGAAAATGCTATTTGCATCAACACTTGCTTCTATTTTATCTGCGACAATCGCTGGATTGTTTATTTAA

Upstream 100 bases:

>100_bases
ACATCTTACGTATGACATAAAACCTCTTTGTTTTATTTTTAGTAATATAACGAGCGTTTTATGTACAAACTTTATACGAA
CTTCTGAAAGGGGCAACTAC

Downstream 100 bases:

>100_bases
AAAAACTCCACCTTACTTCTCTTTTTCTAAAAGAAGTTTGGGTGGAGTTTTTTATCTTCTATATGTATTTTTATGTAAAT
ATCCCAAAAACATATTCCCC

Product: NupC family nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MQYVMSIIGILVVLGLCFALSNNKSKINFRAIAIMIGFQILIGWFMFGTKIGQQIIIFISKVFNKLIKLGTTGVDFLFNG
IQRDFVFFLNVLLIIVFFSALLSIFSYLGVLPFIVRVVGGAISKVTGLPRVESFHAVNSVFFGSSEALIVIKNDLQHFNK
NRMFIICCSAMSSVSASVTASYVMMLDAKYVLAALPLNLFSSLIVCSLLTPVDTKKEDEVVQKFDRTVFGDSFIGAMING
ALDGLKVAGIVAALMIAFIGVMEVVNYVISAASGAMGHAVTLQQIFGYVLAPFAFLMGIPAQDIIPAGGIMGTKIVLNEF
VAILDLKGVAATLSPRTVGIVTVFLISFASISQIGAIVGTIRALSEKQGSIVSKFGWKMLFASTLASILSATIAGLFI

Sequences:

>Translated_398_residues
MQYVMSIIGILVVLGLCFALSNNKSKINFRAIAIMIGFQILIGWFMFGTKIGQQIIIFISKVFNKLIKLGTTGVDFLFNG
IQRDFVFFLNVLLIIVFFSALLSIFSYLGVLPFIVRVVGGAISKVTGLPRVESFHAVNSVFFGSSEALIVIKNDLQHFNK
NRMFIICCSAMSSVSASVTASYVMMLDAKYVLAALPLNLFSSLIVCSLLTPVDTKKEDEVVQKFDRTVFGDSFIGAMING
ALDGLKVAGIVAALMIAFIGVMEVVNYVISAASGAMGHAVTLQQIFGYVLAPFAFLMGIPAQDIIPAGGIMGTKIVLNEF
VAILDLKGVAATLSPRTVGIVTVFLISFASISQIGAIVGTIRALSEKQGSIVSKFGWKMLFASTLASILSATIAGLFI
>Mature_398_residues
MQYVMSIIGILVVLGLCFALSNNKSKINFRAIAIMIGFQILIGWFMFGTKIGQQIIIFISKVFNKLIKLGTTGVDFLFNG
IQRDFVFFLNVLLIIVFFSALLSIFSYLGVLPFIVRVVGGAISKVTGLPRVESFHAVNSVFFGSSEALIVIKNDLQHFNK
NRMFIICCSAMSSVSASVTASYVMMLDAKYVLAALPLNLFSSLIVCSLLTPVDTKKEDEVVQKFDRTVFGDSFIGAMING
ALDGLKVAGIVAALMIAFIGVMEVVNYVISAASGAMGHAVTLQQIFGYVLAPFAFLMGIPAQDIIPAGGIMGTKIVLNEF
VAILDLKGVAATLSPRTVGIVTVFLISFASISQIGAIVGTIRALSEKQGSIVSKFGWKMLFASTLASILSATIAGLFI

Specific function: Transports Nucleosides With A High Affinity Except Guanosine And Deoxyguanosine. Driven By A Proton Motive Force. [C]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI11545853, Length=415, Percent_Identity=25.5421686746988, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI42542381, Length=426, Percent_Identity=25.8215962441315, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI227116277, Length=418, Percent_Identity=25.1196172248804, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788737, Length=392, Percent_Identity=40.5612244897959, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1788485, Length=418, Percent_Identity=31.5789473684211, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788488, Length=417, Percent_Identity=32.3741007194245, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17560276, Length=394, Percent_Identity=23.8578680203046, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71991794, Length=394, Percent_Identity=22.5888324873096, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45552519, Length=394, Percent_Identity=24.8730964467005, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45552517, Length=394, Percent_Identity=24.8730964467005, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19921868, Length=394, Percent_Identity=24.8730964467005, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281360430, Length=358, Percent_Identity=26.2569832402235, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 42689; Mature: 42689

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYVMSIIGILVVLGLCFALSNNKSKINFRAIAIMIGFQILIGWFMFGTKIGQQIIIFIS
CHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVFNKLIKLGTTGVDFLFNGIQRDFVFFLNVLLIIVFFSALLSIFSYLGVLPFIVRVVGG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AISKVTGLPRVESFHAVNSVFFGSSEALIVIKNDLQHFNKNRMFIICCSAMSSVSASVTA
HHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECHHHHCCCCCEEEEEECHHHHHHHHHHH
SYVMMLDAKYVLAALPLNLFSSLIVCSLLTPVDTKKEDEVVQKFDRTVFGDSFIGAMING
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
ALDGLKVAGIVAALMIAFIGVMEVVNYVISAASGAMGHAVTLQQIFGYVLAPFAFLMGIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
AQDIIPAGGIMGTKIVLNEFVAILDLKGVAATLSPRTVGIVTVFLISFASISQIGAIVGT
HHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IRALSEKQGSIVSKFGWKMLFASTLASILSATIAGLFI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8550462; 8867804; 9384377 [H]