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The map label for this gene is purK [H]

Identifier: 49183304

GI number: 49183304

Start: 296037

End: 297188

Strand: Direct

Name: purK [H]

Synonym: BAS0276

Alternate gene names: 49183304

Gene position: 296037-297188 (Clockwise)

Preceding gene: 49183303

Following gene: 49183305

Centisome position: 5.66

GC content: 37.93

Gene sequence:

>1152_bases
ATGACGAGAATCATTTTACCTGGAAAAACAATCGGCATTATTGGAGGCGGCCAGCTAGGAAGAATGATGGCATTGGCAGC
TAAGGAAATGGGATATAAAATTGCTGTTTTAGATCCTACAAAAAACTCACCATGTGCACAAGTTGCTGATATTGAAATTG
TTGCATCATATGACGATTTAAAAGCAATTCAGCATTTAGCAGAGATCAGTGATGTTGTCACATATGAATTTGAGAATATT
GATTATAGATGTTTACAATGGCTTGAAAAACATGCTTACTTACCGCAAGGCAGTCAGTTGTTAAGTAAAACGCAAAATCG
TTTTACAGAAAAAAATGCAATAGAAAAAGCGGGATTACCAGTAGCAACGTATAGATTGGTTCAAAATCAAGAGCAGCTTA
CTGAAGCAATCGCTGAGTTATCATATCCTTCCGTCTTAAAAACAACGACAGGTGGATATGATGGGAAAGGGCAAGTTGTT
TTAAGAAGTGAAGCTGACGTTGATGAAGCGCGAAAGCTTGCGAATGCAGCAGAGTGTATTTTAGAGAAATGGGTGCCTTT
TGAAAAAGAAGTATCAGTTATCGTAATTCGTAGTGTAAGTGGTGAAACGAAAGTATTTCCGGTAGCGGAAAATATTCATG
TAAATAACATTTTGCATGAATCCATCGTTCCAGCTCGCATTACAGAAGAACTTTCTCAAAAAGCAATTGCTTATGCAAAA
GTGCTAGCGGATGAACTAGAACTTGTGGGAACACTAGCGGTAGAGATGTTTGCTACAGCCGATGGTGAGATTTACATTAA
TGAATTGGCGCCAAGACCTCACAATTCAGGACACTATACACAGGATGCATGTGAAACGAGTCAATTCGGTCAACATATTC
GAGCAATCTGTAATTTACCTCTTGGAGAAACAAATTTGTTAAAACCAGTTGTCATGGTAAACATTTTAGGCGAACATATA
GAAGGGGTCCTAAGACAAGTGAATAGATTAACCGGGTGCTATTTACACTTGTATGGAAAAGAAGAAGCAAAAGCGCAGCG
AAAAATGGGGCATGTTAATATTTTAAATGATAATATTGAAGTCGCTCTAGAAAAAGCAAAGAGTTTGCATATTTGGGACC
ATCAAGAACAACTGTTGGAGGGAAAAAGATGA

Upstream 100 bases:

>100_bases
CTGCACAAATACTTGGATCATTCCATGATGACATACATGATGCATTAGAATTGAGAAGAGAAGCAATTGAAAAAGATGTG
CGCGAAGGTAGTGAGCTAGT

Downstream 100 bases:

>100_bases
TTAGTCGTTATACACGCCCAGAAATGGGTGCGATTTGGACGGAAGAGAACAAATTTAAAGCGTGGTTAGAAGTTGAGATT
TTAGCTTGTGAAGCATGGGC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKNSPCAQVADIEIVASYDDLKAIQHLAEISDVVTYEFENI
DYRCLQWLEKHAYLPQGSQLLSKTQNRFTEKNAIEKAGLPVATYRLVQNQEQLTEAIAELSYPSVLKTTTGGYDGKGQVV
LRSEADVDEARKLANAAECILEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAK
VLADELELVGTLAVEMFATADGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLPLGETNLLKPVVMVNILGEHI
EGVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIEVALEKAKSLHIWDHQEQLLEGKR

Sequences:

>Translated_383_residues
MTRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKNSPCAQVADIEIVASYDDLKAIQHLAEISDVVTYEFENI
DYRCLQWLEKHAYLPQGSQLLSKTQNRFTEKNAIEKAGLPVATYRLVQNQEQLTEAIAELSYPSVLKTTTGGYDGKGQVV
LRSEADVDEARKLANAAECILEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAK
VLADELELVGTLAVEMFATADGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLPLGETNLLKPVVMVNILGEHI
EGVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIEVALEKAKSLHIWDHQEQLLEGKR
>Mature_382_residues
TRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKNSPCAQVADIEIVASYDDLKAIQHLAEISDVVTYEFENID
YRCLQWLEKHAYLPQGSQLLSKTQNRFTEKNAIEKAGLPVATYRLVQNQEQLTEAIAELSYPSVLKTTTGGYDGKGQVVL
RSEADVDEARKLANAAECILEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAKV
LADELELVGTLAVEMFATADGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLPLGETNLLKPVVMVNILGEHIE
GVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIEVALEKAKSLHIWDHQEQLLEGKR

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=351, Percent_Identity=31.6239316239316, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788155, Length=371, Percent_Identity=26.1455525606469, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324702, Length=368, Percent_Identity=36.1413043478261, Blast_Score=220, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42517; Mature: 42386

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKNSPCAQVADIEIVASYDDL
CCEEEECCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECEEEEECHHHH
KAIQHLAEISDVVTYEFENIDYRCLQWLEKHAYLPQGSQLLSKTQNRFTEKNAIEKAGLP
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
VATYRLVQNQEQLTEAIAELSYPSVLKTTTGGYDGKGQVVLRSEADVDEARKLANAAECI
HHHHHHHHCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAK
HHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
VLADELELVGTLAVEMFATADGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLP
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
LGETNLLKPVVMVNILGEHIEGVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEECCCHH
VALEKAKSLHIWDHQEQLLEGKR
HHHHHHCCCEEECCHHHHHCCCC
>Mature Secondary Structure 
TRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKNSPCAQVADIEIVASYDDL
CEEEECCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECEEEEECHHHH
KAIQHLAEISDVVTYEFENIDYRCLQWLEKHAYLPQGSQLLSKTQNRFTEKNAIEKAGLP
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
VATYRLVQNQEQLTEAIAELSYPSVLKTTTGGYDGKGQVVLRSEADVDEARKLANAAECI
HHHHHHHHCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAK
HHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
VLADELELVGTLAVEMFATADGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLP
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
LGETNLLKPVVMVNILGEHIEGVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEECCCHH
VALEKAKSLHIWDHQEQLLEGKR
HHHHHHCCCEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]