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The map label for this gene is ykvZ [H]

Identifier: 49183241

GI number: 49183241

Start: 207750

End: 208727

Strand: Direct

Name: ykvZ [H]

Synonym: BAS0208

Alternate gene names: 49183241

Gene position: 207750-208727 (Clockwise)

Preceding gene: 49183240

Following gene: 49183242

Centisome position: 3.97

GC content: 36.4

Gene sequence:

>978_bases
ATGGCTAATATTAAAGATATTGCAAAAATGGCGGGAGTTTCAGTTACGACTGTTTCGAGAGTGTTGAATGATCATCCGTA
TGTAAGTGAAGAAAAAAGGAAAGCGGTTATAGAGATAGTTGAGAAGTTGAATTACTCACAAAACGCAAATGCTGTTCATT
TATCAAAAGGAAAGACGAATATTGTTGGTGTGATTCTCCCTTACATCAATCACCCGAGCTTCGATGCAATGGTAGGGGGA
ATGATGGAGGGAGCTTTAACTCATAACTACAGGGTGCTACTTTGCCAAACGAATTATAATAAAAAAGAAGAAATGAAAAG
TTTACATATGTTAAAAACGAAACAATTGGATGGTCTTATTATTTGTTCACGTGCAAATGATTGGGAAATAATAGAACCGT
ATGCTTCTTACGGTACAATCATTGCTTGTGAAGATAATGATATTTCAAACATCTCAAGTGTATATACAAATCATTCGGCA
GCTTTCCAGTTAGGAATGAATCACCTGATTGAAAAAGGTTATAAAAAAATTGGTTATTGTACGGGAAGAAAGCTAGGACC
GAGTAGTCAAAAGCGTTTTGATGTGTATAAACAGCAATTGCAATCTATAGATGAAGAAGTGAATGAAGAATGGATTTTCA
CAGAATGTTTTACATTAGAAGATGGTGTGAGAGTCGCTCATAAGTTAAAAGGTATGCAGAATCTCCCTGAAGCGTTAATA
GTAGCAGGAGATGAAGTTGCGATTGGGGTTATGACGGAAGTTGGGAAGTTGGGTATTCAAGTTCCTGAGGACTTAGCGAT
TATTGGTTTAGATAACCAACCTATTTCGCAAGTGTTGCAACTTACAACCATTGATCAAAATTTGAAGGAGATAGGGAAAA
CAGCTTTTGAAATGTTTTACCGGCATATAAGTGACAAGAGCTCTAAACAAGAAAAGGTGGAAATTCCATATGAACTTGTG
GAGCGATCTACAGTGTAA

Upstream 100 bases:

>100_bases
GAAAATTACACTTTTGTAAGAAAAACGAATGAAGATGTAATGATGAGAAGGTTTGACATATGTTCATGGAATTTATTAAT
TTAAAAGTAGAGGTGAGTGT

Downstream 100 bases:

>100_bases
TTTTAATCCGTTATGTGTATAAGTACATAACGGATTATTTTTTTGAAATATCTTTGACAGGAAACGCGTTTCATACTTTA
TAAAGGACTTAAACCGGTTT

Product: LacI family sugar-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MANIKDIAKMAGVSVTTVSRVLNDHPYVSEEKRKAVIEIVEKLNYSQNANAVHLSKGKTNIVGVILPYINHPSFDAMVGG
MMEGALTHNYRVLLCQTNYNKKEEMKSLHMLKTKQLDGLIICSRANDWEIIEPYASYGTIIACEDNDISNISSVYTNHSA
AFQLGMNHLIEKGYKKIGYCTGRKLGPSSQKRFDVYKQQLQSIDEEVNEEWIFTECFTLEDGVRVAHKLKGMQNLPEALI
VAGDEVAIGVMTEVGKLGIQVPEDLAIIGLDNQPISQVLQLTTIDQNLKEIGKTAFEMFYRHISDKSSKQEKVEIPYELV
ERSTV

Sequences:

>Translated_325_residues
MANIKDIAKMAGVSVTTVSRVLNDHPYVSEEKRKAVIEIVEKLNYSQNANAVHLSKGKTNIVGVILPYINHPSFDAMVGG
MMEGALTHNYRVLLCQTNYNKKEEMKSLHMLKTKQLDGLIICSRANDWEIIEPYASYGTIIACEDNDISNISSVYTNHSA
AFQLGMNHLIEKGYKKIGYCTGRKLGPSSQKRFDVYKQQLQSIDEEVNEEWIFTECFTLEDGVRVAHKLKGMQNLPEALI
VAGDEVAIGVMTEVGKLGIQVPEDLAIIGLDNQPISQVLQLTTIDQNLKEIGKTAFEMFYRHISDKSSKQEKVEIPYELV
ERSTV
>Mature_324_residues
ANIKDIAKMAGVSVTTVSRVLNDHPYVSEEKRKAVIEIVEKLNYSQNANAVHLSKGKTNIVGVILPYINHPSFDAMVGGM
MEGALTHNYRVLLCQTNYNKKEEMKSLHMLKTKQLDGLIICSRANDWEIIEPYASYGTIIACEDNDISNISSVYTNHSAA
FQLGMNHLIEKGYKKIGYCTGRKLGPSSQKRFDVYKQQLQSIDEEVNEEWIFTECFTLEDGVRVAHKLKGMQNLPEALIV
AGDEVAIGVMTEVGKLGIQVPEDLAIIGLDNQPISQVLQLTTIDQNLKEIGKTAFEMFYRHISDKSSKQEKVEIPYELVE
RSTV

Specific function: Repressor That Binds To The Purf Operator And Coregulates Other Genes For De Novo Purine Nucleotide Synthesis. It Is Involved In Regulation Of Purb, Purc, Purek, Purhd, Purl, Purmn And Guaba Expression. Binds Hypoxanthine And Guanine As Inducers. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=28.2282282282282, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=27.027027027027, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790369, Length=300, Percent_Identity=31.3333333333333, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=28.75, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788474, Length=314, Percent_Identity=27.7070063694268, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=27.891156462585, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786540, Length=334, Percent_Identity=26.0479041916168, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789068, Length=281, Percent_Identity=27.7580071174377, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=22.6708074534161, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790715, Length=339, Percent_Identity=22.1238938053097, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787906, Length=217, Percent_Identity=23.963133640553, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=338, Percent_Identity=21.8934911242604, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786268, Length=304, Percent_Identity=22.0394736842105, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36446; Mature: 36315

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIKDIAKMAGVSVTTVSRVLNDHPYVSEEKRKAVIEIVEKLNYSQNANAVHLSKGKTN
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
IVGVILPYINHPSFDAMVGGMMEGALTHNYRVLLCQTNYNKKEEMKSLHMLKTKQLDGLI
EEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEE
ICSRANDWEIIEPYASYGTIIACEDNDISNISSVYTNHSAAFQLGMNHLIEKGYKKIGYC
EEECCCCCCEECCHHCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
TGRKLGPSSQKRFDVYKQQLQSIDEEVNEEWIFTECFTLEDGVRVAHKLKGMQNLPEALI
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCHHEE
VAGDEVAIGVMTEVGKLGIQVPEDLAIIGLDNQPISQVLQLTTIDQNLKEIGKTAFEMFY
EECCCEEEHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHISDKSSKQEKVEIPYELVERSTV
HHHCCCCCCCHHCCCCHHHHCCCCC
>Mature Secondary Structure 
ANIKDIAKMAGVSVTTVSRVLNDHPYVSEEKRKAVIEIVEKLNYSQNANAVHLSKGKTN
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
IVGVILPYINHPSFDAMVGGMMEGALTHNYRVLLCQTNYNKKEEMKSLHMLKTKQLDGLI
EEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEE
ICSRANDWEIIEPYASYGTIIACEDNDISNISSVYTNHSAAFQLGMNHLIEKGYKKIGYC
EEECCCCCCEECCHHCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
TGRKLGPSSQKRFDVYKQQLQSIDEEVNEEWIFTECFTLEDGVRVAHKLKGMQNLPEALI
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCHHEE
VAGDEVAIGVMTEVGKLGIQVPEDLAIIGLDNQPISQVLQLTTIDQNLKEIGKTAFEMFY
EECCCEEEHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHISDKSSKQEKVEIPYELVERSTV
HHHCCCCCCCHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]