Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 49183065

GI number: 49183065

Start: 36640

End: 37623

Strand: Direct

Name: holB [H]

Synonym: BAS0030

Alternate gene names: 49183065

Gene position: 36640-37623 (Clockwise)

Preceding gene: 49183064

Following gene: 49183066

Centisome position: 0.7

GC content: 36.99

Gene sequence:

>984_bases
ATGACGAAGACGTGGGAGCAGCTTTCTGCTATACAACCCATCGGTGTAAAAATGTTGATGAATAGCATCGCAAAAGAGCG
TATATCCCACGCTTACTTGTTAGAAGGAGAGAAAGGGACAGGAAAGTTTGCGACAGCAATTCAAATGGCAAAGAGTTTCC
TCTGTTCTCAGCGGAATAGGGTAGAGCCTTGTCATGTATGTACAAATTGTAAGCGAATTGATTCAGGTAACCACCCGAAC
TTACATATTGTAAAACCAGACGGATTATCTATTAAGAAGCAACAAATTCATGATTTACAAGAAGAGTTTTCCAAAACAGG
ATTAGAAGCAAATAAAAAGGTATATATCATTGAGCACGCAGACCGTATGACAGCAAATGCAGCGAATGCACTCTTGAAAT
TTTTAGAAGAACCAAGTAGTGATACAACAGCTATTTTACTTACTGAACAAAGTCATCAAATTTTAAATACGATTTTATCT
CGCTGTCAAGTTGTTACATTTAGACCGTTACCTACGGAATCTTTAATTAGAAGATTGCAGGATGAAGGTATTACGGTATC
TTTATCGACACTTGCTGCACAACTCACGAATAGTTTTGATGAAGCTTTAACTTTATGTAATGATGAATGGTTTGCACAAG
CACGAGCTTTAGTGATAAAATTATGTGAAGCGCTCGAAAAAGATAAAGCCTCTATTTTTTTTGTACAAGAAAAATGGGGA
AAACACTTTGGAGAGAAAGAGCAATTACAGCAAGGTTTAGATATGTTACTCCTTATTTATAAGGATTTATTATATGTTCA
ACTTGGAGAAGAAGATCGGCTTGTTTTTCGTGAGCAGAAAGAGATGTTTGAATCTTTCTCCTATGCTCAGAAGCGCATTG
TATCAGCTCTCTTTAATATATTAGAGGCAAAAAATAGAATCAACGCTAATGTAAATGCGCAGCTTGTGTTCGAACAGTTA
GTGTTGCGGTTGCAGGAGGGATGA

Upstream 100 bases:

>100_bases
CTGATCAACCAATGGAAAAACTTATAGAAGAAGTTATTCAGGTTATAGAAGATAAATTGTTATAATAGAAGGAAAGACTG
AAATAAGTGAGGTATGCATT

Downstream 100 bases:

>100_bases
CCGTTTTGTATGATGTAGTAGGTGTTCGCTTTAAGAAGGCCGGAAAGGTGTATTACTTTGATCCGAATCAGTTCGATATC
TCTGAAAATGAGTTTGTAAT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGEKGTGKFATAIQMAKSFLCSQRNRVEPCHVCTNCKRIDSGNHPN
LHIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKVYIIEHADRMTANAANALLKFLEEPSSDTTAILLTEQSHQILNTILS
RCQVVTFRPLPTESLIRRLQDEGITVSLSTLAAQLTNSFDEALTLCNDEWFAQARALVIKLCEALEKDKASIFFVQEKWG
KHFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFREQKEMFESFSYAQKRIVSALFNILEAKNRINANVNAQLVFEQL
VLRLQEG

Sequences:

>Translated_327_residues
MTKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGEKGTGKFATAIQMAKSFLCSQRNRVEPCHVCTNCKRIDSGNHPN
LHIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKVYIIEHADRMTANAANALLKFLEEPSSDTTAILLTEQSHQILNTILS
RCQVVTFRPLPTESLIRRLQDEGITVSLSTLAAQLTNSFDEALTLCNDEWFAQARALVIKLCEALEKDKASIFFVQEKWG
KHFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFREQKEMFESFSYAQKRIVSALFNILEAKNRINANVNAQLVFEQL
VLRLQEG
>Mature_326_residues
TKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGEKGTGKFATAIQMAKSFLCSQRNRVEPCHVCTNCKRIDSGNHPNL
HIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKVYIIEHADRMTANAANALLKFLEEPSSDTTAILLTEQSHQILNTILSR
CQVVTFRPLPTESLIRRLQDEGITVSLSTLAAQLTNSFDEALTLCNDEWFAQARALVIKLCEALEKDKASIFFVQEKWGK
HFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFREQKEMFESFSYAQKRIVSALFNILEAKNRINANVNAQLVFEQLV
LRLQEG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=197, Percent_Identity=31.9796954314721, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787341, Length=213, Percent_Identity=29.5774647887324, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37213; Mature: 37082

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGEKGTGKFATAIQMAKSFLCSQRNR
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCC
VEPCHVCTNCKRIDSGNHPNLHIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKVYIIEHA
CCHHHHHCCCHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
DRMTANAANALLKFLEEPSSDTTAILLTEQSHQILNTILSRCQVVTFRPLPTESLIRRLQ
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCEEEEECCCCHHHHHHHHH
DEGITVSLSTLAAQLTNSFDEALTLCNDEWFAQARALVIKLCEALEKDKASIFFVQEKWG
HCCCEEEHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
KHFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFREQKEMFESFSYAQKRIVSALFNI
HCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAKNRINANVNAQLVFEQLVLRLQEG
HHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGEKGTGKFATAIQMAKSFLCSQRNR
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCC
VEPCHVCTNCKRIDSGNHPNLHIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKVYIIEHA
CCHHHHHCCCHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
DRMTANAANALLKFLEEPSSDTTAILLTEQSHQILNTILSRCQVVTFRPLPTESLIRRLQ
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCEEEEECCCCHHHHHHHHH
DEGITVSLSTLAAQLTNSFDEALTLCNDEWFAQARALVIKLCEALEKDKASIFFVQEKWG
HCCCEEEHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
KHFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFREQKEMFESFSYAQKRIVSALFNI
HCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAKNRINANVNAQLVFEQLVLRLQEG
HHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]