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The map label for this gene is yaaO [H]

Identifier: 49183063

GI number: 49183063

Start: 34555

End: 35976

Strand: Direct

Name: yaaO [H]

Synonym: BAS0028

Alternate gene names: 49183063

Gene position: 34555-35976 (Clockwise)

Preceding gene: 49183062

Following gene: 49183064

Centisome position: 0.66

GC content: 35.09

Gene sequence:

>1422_bases
ATGAATCAAAATCGCATACCTTTATATGAAGCATTAATAGAGTTTAAAGAAAGAAGACGGTTATCCTTTCATGTTCCAGG
TCATAAAAATGGTTTAAACTTCCCTCAGGAGGTAGTAGAGGAGTTTAAAGATATACTATCTATCGATGTAACTGAGTTGT
CAGGATTAGATGATTTACATAGTCCGTTTGAATGTATAGATGAGGCACAACAATTATTAGCTGATGTATATGGAGTGAAC
AAAAGTTATTTTTTAATTAATGGTTCAACAGTCGGGAATCTAGCAATGATTTTGTCTTGTTGTGGGGAACATGATATTGT
TCTTGTACAAAGAAATTGTCATAAATCGATTATTAATGGTTTAAAATTAGCGGGAGCAAATCCTATTTTTTTAGATCCAT
GGATTGATGAAGCTTATAACGTACCAGTGGGAATTCATGATGAGATTATTAAGGAAGCGATTGAAAAATACCCAAATGCG
AAAGCGCTCATTTTAACGCATCCTAATTATTATGGTATGGGGATGGATCTAGAGGCAAGCATTGCTTATGCACATACACA
TAAAATCCCTGTGCTAGTAGATGAAGCACATGGGGCTCATTTTTGTTTAGGCGGGGCATTTCCACAGTCAGCGTTAGCAT
ATGGTGCAGATATGGTAGTTCATTCTGCGCATAAAACATTACCCGCGATGACGATGGGATCCTATTTACATATAAATAGT
CGTTTAGTGAAAGAAGAAAAAGTTTCTACTTATTTAAGTATGCTACAATCTAGTAGTCCATCGTATCCAATTATGGCTTC
TCTTGATATTGCACGCTTTACAATAGCACGTATAAAAGAAAAGGGACATGATGAAATTGTCGAGTTTTTACAGGAATTTA
AAGAAGAATTGAGTACTATTCCACAAATAGCTATTTTACAATATCCATTACAAGACGGGCTAAAGATTACCGTACAGACG
CGTTGTCGGTTATCAGGATACGAATTACAGTCTGTATTTGAAAAGGTCGGTATATATACAGAAATGGCAGATCCATATAA
TGTCTTATTTATTTTGCCTTTGCAAGTAAATAAGAAATATATGAAGGCTATAGAGATGATCCGAGTAGCATTGCAATACT
ATGAGGTGAAAGACAAGACGGAATCTATTCGTTATACTTATAAAGGAGAAATTTCTCCCCTGCCGTATACATATAAACAA
CTGGAAGAATACGAAACAAAAGTAGTACCTATAGAAGAAGCAGTTGGGATGGTAGCAGCGGAAATGGTAATTCCGTATCC
ACCTGGTATTCCATTGATTATGTATGGAGAAAGAATTACTTCAGGACATAAAGAACAAATTATGTATTTAGAAAAAGCAG
GTGCTCGTTTTCAAGGTAGCAGGAAATATATGAAAGTGTATGATATAGAAAGTAGGTTTTAG

Upstream 100 bases:

>100_bases
TTCGAAAATTAGAAGTATCATATTTTTAAATAGGCATATGCCTATTATTTTTTTGTTCTGTATAATAGATAAAGAATGAA
ATAATAAGGAAGAGATATAG

Downstream 100 bases:

>100_bases
GATGAAGGGATTATTTGTAACAATTGAAGGTCCAGAAGGTTCGGGTAAAACAACATTAATTCAAAGTTTATTGCCATACT
TTGAACAAAAGGAACAAAAG

Product: lysine decarboxylase

Products: 1,5-Diaminopentane; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNQNRIPLYEALIEFKERRRLSFHVPGHKNGLNFPQEVVEEFKDILSIDVTELSGLDDLHSPFECIDEAQQLLADVYGVN
KSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNA
KALILTHPNYYGMGMDLEASIAYAHTHKIPVLVDEAHGAHFCLGGAFPQSALAYGADMVVHSAHKTLPAMTMGSYLHINS
RLVKEEKVSTYLSMLQSSSPSYPIMASLDIARFTIARIKEKGHDEIVEFLQEFKEELSTIPQIAILQYPLQDGLKITVQT
RCRLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNKKYMKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQ
LEEYETKVVPIEEAVGMVAAEMVIPYPPGIPLIMYGERITSGHKEQIMYLEKAGARFQGSRKYMKVYDIESRF

Sequences:

>Translated_473_residues
MNQNRIPLYEALIEFKERRRLSFHVPGHKNGLNFPQEVVEEFKDILSIDVTELSGLDDLHSPFECIDEAQQLLADVYGVN
KSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNA
KALILTHPNYYGMGMDLEASIAYAHTHKIPVLVDEAHGAHFCLGGAFPQSALAYGADMVVHSAHKTLPAMTMGSYLHINS
RLVKEEKVSTYLSMLQSSSPSYPIMASLDIARFTIARIKEKGHDEIVEFLQEFKEELSTIPQIAILQYPLQDGLKITVQT
RCRLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNKKYMKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQ
LEEYETKVVPIEEAVGMVAAEMVIPYPPGIPLIMYGERITSGHKEQIMYLEKAGARFQGSRKYMKVYDIESRF
>Mature_473_residues
MNQNRIPLYEALIEFKERRRLSFHVPGHKNGLNFPQEVVEEFKDILSIDVTELSGLDDLHSPFECIDEAQQLLADVYGVN
KSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNA
KALILTHPNYYGMGMDLEASIAYAHTHKIPVLVDEAHGAHFCLGGAFPQSALAYGADMVVHSAHKTLPAMTMGSYLHINS
RLVKEEKVSTYLSMLQSSSPSYPIMASLDIARFTIARIKEKGHDEIVEFLQEFKEELSTIPQIAILQYPLQDGLKITVQT
RCRLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNKKYMKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQ
LEEYETKVVPIEEAVGMVAAEMVIPYPPGIPLIMYGERITSGHKEQIMYLEKAGARFQGSRKYMKVYDIESRF

Specific function: Ldc Is Constitutively But Weakly Expressed Under Various Conditions. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1786384, Length=554, Percent_Identity=22.2021660649819, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790573, Length=574, Percent_Identity=22.9965156794425, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI221142684, Length=416, Percent_Identity=24.2788461538462, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786909, Length=315, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18 [C]

Molecular weight: Translated: 53625; Mature: 53625

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNRIPLYEALIEFKERRRLSFHVPGHKNGLNFPQEVVEEFKDILSIDVTELSGLDDLH
CCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHEECHHHHCCCHHHC
SPFECIDEAQQLLADVYGVNKSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIING
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHCC
LKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNAKALILTHPNYYGMGMDLEAS
CEECCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEE
IAYAHTHKIPVLVDEAHGAHFCLGGAFPQSALAYGADMVVHSAHKTLPAMTMGSYLHINS
EEEEEECCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECH
RLVKEEKVSTYLSMLQSSSPSYPIMASLDIARFTIARIKEKGHDEIVEFLQEFKEELSTI
HHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
PQIAILQYPLQDGLKITVQTRCRLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNKKY
CCEEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHCCEEECCCCCEEEEEEEECCCHHH
MKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQLEEYETKVVPIEEAVGMVAA
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHH
EMVIPYPPGIPLIMYGERITSGHKEQIMYLEKAGARFQGSRKYMKVYDIESRF
HHEECCCCCCCEEEECCHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MNQNRIPLYEALIEFKERRRLSFHVPGHKNGLNFPQEVVEEFKDILSIDVTELSGLDDLH
CCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHEECHHHHCCCHHHC
SPFECIDEAQQLLADVYGVNKSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIING
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHCC
LKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNAKALILTHPNYYGMGMDLEAS
CEECCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEE
IAYAHTHKIPVLVDEAHGAHFCLGGAFPQSALAYGADMVVHSAHKTLPAMTMGSYLHINS
EEEEEECCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECH
RLVKEEKVSTYLSMLQSSSPSYPIMASLDIARFTIARIKEKGHDEIVEFLQEFKEELSTI
HHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
PQIAILQYPLQDGLKITVQTRCRLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNKKY
CCEEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHCCEEECCCCCEEEEEEEECCCHHH
MKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQLEEYETKVVPIEEAVGMVAA
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHH
EMVIPYPPGIPLIMYGERITSGHKEQIMYLEKAGARFQGSRKYMKVYDIESRF
HHEECCCCCCCEEEECCHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: H+; L-Lysine [C]

Specific reaction: H+ + L-Lysine --> 1,5-Diaminopentane + CO2 [C]

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]