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The map label for this gene is alkA [H]

Identifier: 46447468

GI number: 46447468

Start: 2191751

End: 2193181

Strand: Reverse

Name: alkA [H]

Synonym: pc1834

Alternate gene names: 46447468

Gene position: 2193181-2191751 (Counterclockwise)

Preceding gene: 46447470

Following gene: 46447462

Centisome position: 90.84

GC content: 38.5

Gene sequence:

>1431_bases
ATGTATTTCAAAGCTTATCTTTCCCGCGATGCACGTTTTGATGGGATTTTTTATGTGGGAGTCCTTTCTACTGGAATTTA
CTGTCGGTGTATTTGTCCTGCGAGAAGACCAAAAGAATCAAATTGTCGCTTTTTCAAAAGTACAGGGGAAGCTGAAAAAG
AATTTTTTCGACCTTGTTTACGCTGTCGCCCAGAGCTAGCACCGGGTCAGTCCCCAGTGGATGATGCTCATCGCATTGCC
AATCTTTTGATTTCTTCTATGCAAGAAGGGTTGTTCGATGAATTAGGACTTGAAGATATTGCCTTAAAATTCAATTTAAG
TTCTCGTCAAATTCGGCGTGTCATTCAGAAAGAATTTGGTGTCTCACCTATTCAATTATTATTAACTCGCCGGTTACTTT
TAGCAAAGCAGTTATTGACGGATACCGATTTGAGTGTGATAGATATTGCTTTTGCTAGTGGATTTTCTAGCCTGCGTCGT
TTCAATGATGCTTTTAAAAAGCATTATCGCATGCCTCCCAACCAATTAAAAAAAGAAGGTCAACGAAAGAAAACTTTTTT
TCCTTCCGCATACATTACGCTGCAATTGAATTATCGTCCCCCCTATGATTGGATAGGTTTTTTAAATTTTTTATCTATTC
GTTCTTTAAAGGGGATAGAGCTAGTTAAAAATAATTGTTATTTACGTACTGTACAGATTAGAGAGTATAAAGGCTGGATT
CACGTTTCTCATGTAGAGGATAAACATTGCCTAAGAGTCAAAATTGCTTCTTCTTTAGTTCCTGTGCTAGCGATATTATT
AGAGCGAATTCGCAATTTTTTTGATTTAAATGCTAGGCCTGATAAAATTTCTGTCCAACTTGAGCAAGATCCCTTTTTGG
CAGAAGAAGTGGCAAAAAATCCAGGTTTGAGAGTTCCTGGAACATTTGACGGATTTGAGCTAGCATTTAGAGCTATTTTA
GGGCAACAAATTACTGTCAAAGCAGCCACTACACTCGCTAGTCGATTTGTAAAAGCATTTGGTGAGGAATTTAAAACTCC
CTTTGCAGAGTTGCATTATTTATGTCCAAGTTCTCAACGCATTTCTTCTTTAAAGTGGGAAGAAATTGCGACTATTGGAA
TTATTCGTGCAAGAGCTCAAACAATAATTGAATTAGCCAAACAAATGTCATCAAATACTCTTAAATTAGAAGCAGGCGTT
AATCTGAGATTGACAATTAAACAATTAACATCGATAGCAGGGATTGGCCAATGGACGGCTCATTATATTGCTTTACGAGC
TCTCCAATGGCCTGATGCGTTTCCAAAAGAAGATGTGGCATTGCGCAAAAAACTTGGCAAAGTAACGGCGAAGCAAGCGG
AGAAATTATCACAAGTGTGGCGTCCGTGGCGCAGCTATGCAACGTTGTACCTGTGGCAAAAAAAGGACTAA

Upstream 100 bases:

>100_bases
CATCAAATCTTTTAACCCTTTCCTAACTTTAATGTCCGAAAGTGGCTAGAAAATTTTCTGAAAATCTTTTACCATAAAAA
AGATGAAGATAGAACAACAA

Downstream 100 bases:

>100_bases
AATAGCTGATTCAAATTTAACGAACTTTAATTAATCATCTAGTTGTTTGAGAATTTTTTCCAAATTTATTCCTTTACCAA
GAATTGGTTTGAATAAATCA

Product: putative DNA-3-methyladenine glycosidase II

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MYFKAYLSRDARFDGIFYVGVLSTGIYCRCICPARRPKESNCRFFKSTGEAEKEFFRPCLRCRPELAPGQSPVDDAHRIA
NLLISSMQEGLFDELGLEDIALKFNLSSRQIRRVIQKEFGVSPIQLLLTRRLLLAKQLLTDTDLSVIDIAFASGFSSLRR
FNDAFKKHYRMPPNQLKKEGQRKKTFFPSAYITLQLNYRPPYDWIGFLNFLSIRSLKGIELVKNNCYLRTVQIREYKGWI
HVSHVEDKHCLRVKIASSLVPVLAILLERIRNFFDLNARPDKISVQLEQDPFLAEEVAKNPGLRVPGTFDGFELAFRAIL
GQQITVKAATTLASRFVKAFGEEFKTPFAELHYLCPSSQRISSLKWEEIATIGIIRARAQTIIELAKQMSSNTLKLEAGV
NLRLTIKQLTSIAGIGQWTAHYIALRALQWPDAFPKEDVALRKKLGKVTAKQAEKLSQVWRPWRSYATLYLWQKKD

Sequences:

>Translated_476_residues
MYFKAYLSRDARFDGIFYVGVLSTGIYCRCICPARRPKESNCRFFKSTGEAEKEFFRPCLRCRPELAPGQSPVDDAHRIA
NLLISSMQEGLFDELGLEDIALKFNLSSRQIRRVIQKEFGVSPIQLLLTRRLLLAKQLLTDTDLSVIDIAFASGFSSLRR
FNDAFKKHYRMPPNQLKKEGQRKKTFFPSAYITLQLNYRPPYDWIGFLNFLSIRSLKGIELVKNNCYLRTVQIREYKGWI
HVSHVEDKHCLRVKIASSLVPVLAILLERIRNFFDLNARPDKISVQLEQDPFLAEEVAKNPGLRVPGTFDGFELAFRAIL
GQQITVKAATTLASRFVKAFGEEFKTPFAELHYLCPSSQRISSLKWEEIATIGIIRARAQTIIELAKQMSSNTLKLEAGV
NLRLTIKQLTSIAGIGQWTAHYIALRALQWPDAFPKEDVALRKKLGKVTAKQAEKLSQVWRPWRSYATLYLWQKKD
>Mature_476_residues
MYFKAYLSRDARFDGIFYVGVLSTGIYCRCICPARRPKESNCRFFKSTGEAEKEFFRPCLRCRPELAPGQSPVDDAHRIA
NLLISSMQEGLFDELGLEDIALKFNLSSRQIRRVIQKEFGVSPIQLLLTRRLLLAKQLLTDTDLSVIDIAFASGFSSLRR
FNDAFKKHYRMPPNQLKKEGQRKKTFFPSAYITLQLNYRPPYDWIGFLNFLSIRSLKGIELVKNNCYLRTVQIREYKGWI
HVSHVEDKHCLRVKIASSLVPVLAILLERIRNFFDLNARPDKISVQLEQDPFLAEEVAKNPGLRVPGTFDGFELAFRAIL
GQQITVKAATTLASRFVKAFGEEFKTPFAELHYLCPSSQRISSLKWEEIATIGIIRARAQTIIELAKQMSSNTLKLEAGV
NLRLTIKQLTSIAGIGQWTAHYIALRALQWPDAFPKEDVALRKKLGKVTAKQAEKLSQVWRPWRSYATLYLWQKKD

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=278, Percent_Identity=33.8129496402878, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI1788542, Length=182, Percent_Identity=26.9230769230769, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790339, Length=98, Percent_Identity=30.6122448979592, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 54634; Mature: 54634

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00516 ALKYLBASE_DNA_GLYCOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFKAYLSRDARFDGIFYVGVLSTGIYCRCICPARRPKESNCRFFKSTGEAEKEFFRPCL
CCCHHHHCCCCCCCCEEEEEHHCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHH
RCRPELAPGQSPVDDAHRIANLLISSMQEGLFDELGLEDIALKFNLSSRQIRRVIQKEFG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHC
VSPIQLLLTRRLLLAKQLLTDTDLSVIDIAFASGFSSLRRFNDAFKKHYRMPPNQLKKEG
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
QRKKTFFPSAYITLQLNYRPPYDWIGFLNFLSIRSLKGIELVKNNCYLRTVQIREYKGWI
CHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEEEEEECCEE
HVSHVEDKHCLRVKIASSLVPVLAILLERIRNFFDLNARPDKISVQLEQDPFLAEEVAKN
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHCC
PGLRVPGTFDGFELAFRAILGQQITVKAATTLASRFVKAFGEEFKTPFAELHYLCPSSQR
CCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHH
ISSLKWEEIATIGIIRARAQTIIELAKQMSSNTLKLEAGVNLRLTIKQLTSIAGIGQWTA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHCCHHHHH
HYIALRALQWPDAFPKEDVALRKKLGKVTAKQAEKLSQVWRPWRSYATLYLWQKKD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
>Mature Secondary Structure
MYFKAYLSRDARFDGIFYVGVLSTGIYCRCICPARRPKESNCRFFKSTGEAEKEFFRPCL
CCCHHHHCCCCCCCCEEEEEHHCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHH
RCRPELAPGQSPVDDAHRIANLLISSMQEGLFDELGLEDIALKFNLSSRQIRRVIQKEFG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHC
VSPIQLLLTRRLLLAKQLLTDTDLSVIDIAFASGFSSLRRFNDAFKKHYRMPPNQLKKEG
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
QRKKTFFPSAYITLQLNYRPPYDWIGFLNFLSIRSLKGIELVKNNCYLRTVQIREYKGWI
CHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEEEEEECCEE
HVSHVEDKHCLRVKIASSLVPVLAILLERIRNFFDLNARPDKISVQLEQDPFLAEEVAKN
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHCC
PGLRVPGTFDGFELAFRAILGQQITVKAATTLASRFVKAFGEEFKTPFAELHYLCPSSQR
CCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHH
ISSLKWEEIATIGIIRARAQTIIELAKQMSSNTLKLEAGVNLRLTIKQLTSIAGIGQWTA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHCCHHHHH
HYIALRALQWPDAFPKEDVALRKKLGKVTAKQAEKLSQVWRPWRSYATLYLWQKKD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]