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The map label for this gene is nagA [H]

Identifier: 46447459

GI number: 46447459

Start: 2179640

End: 2181292

Strand: Reverse

Name: nagA [H]

Synonym: pc1825

Alternate gene names: 46447459

Gene position: 2181292-2179640 (Counterclockwise)

Preceding gene: 46447461

Following gene: 46447458

Centisome position: 90.34

GC content: 33.76

Gene sequence:

>1653_bases
ATGTTTACTATCATTTCAAAAAAGTTTCCATTCATTTACAAAAAAAATTTATTATTATTATTTTTTTTGTTTGTTTCATT
AAAGGCAGAAAATACTGTTCCTAAGCTTGAAGATTTAACTTTAGAAGAAAAAATTGGTCAATTATTAATCGTTCATTTTC
GAGGGGAAGATTGTAATAAAGAAGCTCTTCAAATGATATATGAAGCCCATGTAGGAGGAGTTATTTATTATCCATGGTCA
AATGGTTTAAACTCTCCTCTTCAAATACAACATTTAAGCGCCCATTTACAAGCTGCGGCATTTCAAAAAAAAAATAAAAT
CCCTTTATTAATTTCAGTGGATCAAGAAGGGGGGGTTGTCAATCGTTTAACCAAAGGGATGACTCTTTTTCCTGGTAACT
ATGCTTTAGGTCAAACGAAGGATCCTCAATTAGCCTTTGAGTATGCGAGTGCGTCAGCAAATGAACTTCTAGCGATGGGA
ATCAATATGAATTTGGCTCCTGTTATAGATGTCAACACTCAACCTTGTAATCCAGTCATTGGGATTCGCTCTTTTAGTAC
TTGTCCAGATGAAATTGTTCGATTTGGTTCAAAAGTAGTTGAAGGATTTAACAAGGCTGGATTGATTGCAACTCTAAAGC
ATTTTCCTGGCCATGGTGATACAAACTCTGATTCGCATCATACTTTGCCTATCGTTAACAAAACGCTTGTTGAATTAGAA
AAAGTCGAACTTGTTCCCTTTAAAGCCCTCGCTTCTCAAACACCTGTTGTCATGACTGCTCATTTGCTTGTTCCTGCACT
TGATGCTTTGAATTGCGTAACATTTTCGAAATCTGCGATTGATGTTCTAAGAAAAGAGATCGGAGAAAAAGGAATCATTT
TAACAGATTCGCTTGTAATGCAAGGGGTATTGCAAGATGGCAAAACTGTAGAACAAGCTGCTTTAAAAAGCTTACAAGCA
GGACATGATCTCTTACTGCTAGGAGGTAAACGACTTTTAAATGAATCTAGCGAGTTTGAGTTGGAAGCTAAAGACGTTTT
AAAAATTCACCAATTTCTTGTTAATGCAGTTAGACAAAATCTTATTTCTGAAGCGAGAATTGATATCTCGGTAAAAAGGA
TTCTTGCTCTTAAGGAATCCTATCATCTTTTTCAATCAGAAGAAGATGATTTACAATTGAGGAGAAGAGAACTTTCCCTT
CAAAAAAATCAAGCTTTGGCAAAAAACATTGCAGAAAAAGCCTTGCATAATCTAAATGAGGATTTAAAAATTTCATTTCA
GAATCCAAGGGTTACTTTAATTGCACCTGACATTTTAAAAGAAGAATTAGAAAAAACAGATTGGTTAACACCTAATCAAA
ATGTGCAAGTCATTTTGTTTGACGCCAATTTAAAAGAATTTCAAACCGTTTTGGAAAAGTGTCAAGAAATTAATGTTTGT
GTATTCTTTTTTTACAATCATAAAAAATATCCGAATCATTTAGATTTCTTCAACTTTATTAAAGCTCATAAAAATAGCGA
AATGATCGCTGTCGTTGTTGCCGATTCCAATATCCAACATGATTTGGCAGATGTGACTCTATATACTTACAGTCCTTGCG
CGGTTTCACTTCAATGTGCTAAAGAATATTTAATGACAATTTTTGAAGAATAA

Upstream 100 bases:

>100_bases
AAATTTTTACGACTAATAAGAAGATTTTTAGAGAGACACGAATTGTTTGTTTTTTCCAATATCTGGTATAAGGATTATTT
TTCTTCTTTAGGTCTTTTTT

Downstream 100 bases:

>100_bases
AAGGAGAAAATGAAAAAAGTATTGTTGCGTCTATTACTTTTAATGTTTCTCATCTCTTTTAATTGTTCTGGTTTGGCAGT
AGTCATCAATCCTGCTTTTT

Product: putative beta-N-acetylglucosaminidase

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MFTIISKKFPFIYKKNLLLLFFLFVSLKAENTVPKLEDLTLEEKIGQLLIVHFRGEDCNKEALQMIYEAHVGGVIYYPWS
NGLNSPLQIQHLSAHLQAAAFQKKNKIPLLISVDQEGGVVNRLTKGMTLFPGNYALGQTKDPQLAFEYASASANELLAMG
INMNLAPVIDVNTQPCNPVIGIRSFSTCPDEIVRFGSKVVEGFNKAGLIATLKHFPGHGDTNSDSHHTLPIVNKTLVELE
KVELVPFKALASQTPVVMTAHLLVPALDALNCVTFSKSAIDVLRKEIGEKGIILTDSLVMQGVLQDGKTVEQAALKSLQA
GHDLLLLGGKRLLNESSEFELEAKDVLKIHQFLVNAVRQNLISEARIDISVKRILALKESYHLFQSEEDDLQLRRRELSL
QKNQALAKNIAEKALHNLNEDLKISFQNPRVTLIAPDILKEELEKTDWLTPNQNVQVILFDANLKEFQTVLEKCQEINVC
VFFFYNHKKYPNHLDFFNFIKAHKNSEMIAVVVADSNIQHDLADVTLYTYSPCAVSLQCAKEYLMTIFEE

Sequences:

>Translated_550_residues
MFTIISKKFPFIYKKNLLLLFFLFVSLKAENTVPKLEDLTLEEKIGQLLIVHFRGEDCNKEALQMIYEAHVGGVIYYPWS
NGLNSPLQIQHLSAHLQAAAFQKKNKIPLLISVDQEGGVVNRLTKGMTLFPGNYALGQTKDPQLAFEYASASANELLAMG
INMNLAPVIDVNTQPCNPVIGIRSFSTCPDEIVRFGSKVVEGFNKAGLIATLKHFPGHGDTNSDSHHTLPIVNKTLVELE
KVELVPFKALASQTPVVMTAHLLVPALDALNCVTFSKSAIDVLRKEIGEKGIILTDSLVMQGVLQDGKTVEQAALKSLQA
GHDLLLLGGKRLLNESSEFELEAKDVLKIHQFLVNAVRQNLISEARIDISVKRILALKESYHLFQSEEDDLQLRRRELSL
QKNQALAKNIAEKALHNLNEDLKISFQNPRVTLIAPDILKEELEKTDWLTPNQNVQVILFDANLKEFQTVLEKCQEINVC
VFFFYNHKKYPNHLDFFNFIKAHKNSEMIAVVVADSNIQHDLADVTLYTYSPCAVSLQCAKEYLMTIFEE
>Mature_550_residues
MFTIISKKFPFIYKKNLLLLFFLFVSLKAENTVPKLEDLTLEEKIGQLLIVHFRGEDCNKEALQMIYEAHVGGVIYYPWS
NGLNSPLQIQHLSAHLQAAAFQKKNKIPLLISVDQEGGVVNRLTKGMTLFPGNYALGQTKDPQLAFEYASASANELLAMG
INMNLAPVIDVNTQPCNPVIGIRSFSTCPDEIVRFGSKVVEGFNKAGLIATLKHFPGHGDTNSDSHHTLPIVNKTLVELE
KVELVPFKALASQTPVVMTAHLLVPALDALNCVTFSKSAIDVLRKEIGEKGIILTDSLVMQGVLQDGKTVEQAALKSLQA
GHDLLLLGGKRLLNESSEFELEAKDVLKIHQFLVNAVRQNLISEARIDISVKRILALKESYHLFQSEEDDLQLRRRELSL
QKNQALAKNIAEKALHNLNEDLKISFQNPRVTLIAPDILKEELEKTDWLTPNQNVQVILFDANLKEFQTVLEKCQEINVC
VFFFYNHKKYPNHLDFFNFIKAHKNSEMIAVVVADSNIQHDLADVTLYTYSPCAVSLQCAKEYLMTIFEE

Specific function: Hydrolyses the beta-1,4-glycosidic bond between N-acetylglucosamine and anhydro-N-acetylmuramic acid in cell wall degradation products following their importation into the cell during the process for recycling cell wall muropeptides [C]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=344, Percent_Identity=28.4883720930233, Blast_Score=132, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR002772
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3; PF01915 Glyco_hydro_3_C [H]

EC number: 3.2.1.52

Molecular weight: Translated: 61735; Mature: 61735

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTIISKKFPFIYKKNLLLLFFLFVSLKAENTVPKLEDLTLEEKIGQLLIVHFRGEDCNK
CEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHCCCEEEEEEECCCCCCH
EALQMIYEAHVGGVIYYPWSNGLNSPLQIQHLSAHLQAAAFQKKNKIPLLISVDQEGGVV
HHHHHHHHHHCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHH
NRLTKGMTLFPGNYALGQTKDPQLAFEYASASANELLAMGINMNLAPVIDVNTQPCNPVI
HHHHCCCEECCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCEEEEECCCCCCCCEE
GIRSFSTCPDEIVRFGSKVVEGFNKAGLIATLKHFPGHGDTNSDSHHTLPIVNKTLVELE
ECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KVELVPFKALASQTPVVMTAHLLVPALDALNCVTFSKSAIDVLRKEIGEKGIILTDSLVM
HHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEHHHHH
QGVLQDGKTVEQAALKSLQAGHDLLLLGGKRLLNESSEFELEAKDVLKIHQFLVNAVRQN
HHHHHCCCHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEECHHHHHHHHHHHHHHHHHH
LISEARIDISVKRILALKESYHLFQSEEDDLQLRRRELSLQKNQALAKNIAEKALHNLNE
HHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLKISFQNPRVTLIAPDILKEELEKTDWLTPNQNVQVILFDANLKEFQTVLEKCQEINVC
CEEEEECCCEEEEEEHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEE
VFFFYNHKKYPNHLDFFNFIKAHKNSEMIAVVVADSNIQHDLADVTLYTYSPCAVSLQCA
EEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHEEEEEECCCCEEHHHH
KEYLMTIFEE
HHHHHHHHCC
>Mature Secondary Structure
MFTIISKKFPFIYKKNLLLLFFLFVSLKAENTVPKLEDLTLEEKIGQLLIVHFRGEDCNK
CEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHCCCEEEEEEECCCCCCH
EALQMIYEAHVGGVIYYPWSNGLNSPLQIQHLSAHLQAAAFQKKNKIPLLISVDQEGGVV
HHHHHHHHHHCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHH
NRLTKGMTLFPGNYALGQTKDPQLAFEYASASANELLAMGINMNLAPVIDVNTQPCNPVI
HHHHCCCEECCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCEEEEECCCCCCCCEE
GIRSFSTCPDEIVRFGSKVVEGFNKAGLIATLKHFPGHGDTNSDSHHTLPIVNKTLVELE
ECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KVELVPFKALASQTPVVMTAHLLVPALDALNCVTFSKSAIDVLRKEIGEKGIILTDSLVM
HHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEHHHHH
QGVLQDGKTVEQAALKSLQAGHDLLLLGGKRLLNESSEFELEAKDVLKIHQFLVNAVRQN
HHHHHCCCHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEECHHHHHHHHHHHHHHHHHH
LISEARIDISVKRILALKESYHLFQSEEDDLQLRRRELSLQKNQALAKNIAEKALHNLNE
HHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLKISFQNPRVTLIAPDILKEELEKTDWLTPNQNVQVILFDANLKEFQTVLEKCQEINVC
CEEEEECCCEEEEEEHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEE
VFFFYNHKKYPNHLDFFNFIKAHKNSEMIAVVVADSNIQHDLADVTLYTYSPCAVSLQCA
EEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHEEEEEECCCCEEHHHH
KEYLMTIFEE
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8011666; 9274029; 9384377 [H]