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The map label for this gene is yohI [H]

Identifier: 46447213

GI number: 46447213

Start: 1895503

End: 1896483

Strand: Reverse

Name: yohI [H]

Synonym: pc1579

Alternate gene names: 46447213

Gene position: 1896483-1895503 (Counterclockwise)

Preceding gene: 46447214

Following gene: 46447209

Centisome position: 78.55

GC content: 37.92

Gene sequence:

>981_bases
ATGACTTTTCTTCCTAAAAATTCTTTTGGTTGTCCTTATTTGATCCTTGCCCCTATGGAAGGAGTCGGAGATCGCAGTTT
TAGAAAAGCTATGGCATCAGTGGGGGGATTTGACGAAGCTTGCACAGAATTTATGCGAGTGCCCAGTAATGCTCATGTTC
CTAGTTTAGCCAATCGTTATGAAGCGAATGAAACTTTTCCCATTCCCCAAGCTGCGCAATTGATGGGTTCGGATCCAGAG
CTTATGGCAGAAATGGCAAGAGCTGTAGAAAGGCGAGGAGCTCAGCGTATTGATTTAAATTGTGGTTGTCCCTCTAATAC
AGTAACCGGAAGAGGGGCTGGATCTAGTCTACTCAAAGAACCTGATCATTTATATAAAGTTGCTAAATCAATGGTAGAGG
CTGTAAATGTACCTGTAACAGCAAAGCTTCGTTCAGGTTTTACAGACACCTCTTTATTTCATGATAATCTCCTTGCAGCG
CAAGCCAGCGGAATCAAATATTTGACACTGCATCCTAGAACAAAAGTTGATGGATATGGACCTCCTGCGAAATGGGAATT
AATAGCACAAGCTAAGCAACTCTTAAAAATTCCTGTTGTGGGAAACGGCGATATTTTAACTGTCCAAGATGCATTGAACA
TGCTTAAGCAGACAAAATGTGATGGTTTAATGATTGGAAGGGGGAGTGTGATTAATCCTTTTATTTTTCATGAAATTAAA
AGTCATTTTTCCGCTCAGCCCTTTGAAAAAAAATGGGATGTATTTAGCCGCTATATCGACGTTTTTACTGAAGATTTAAA
AGAAATGTCTGTCAAAGGACAGCTTAATAAGTTAAAACAATTAATGAGTTTTTTATTTAAATTTAATAGTAAATTAAGAG
AAAAAAAACAAAAAATTCTTACTCACCAGACGGTAAATGCCCAAAGTTTTTTATCTTTTGCCTTGGAAAACTACCAAGAA
GATTGGATGGAAGCCAATTAA

Upstream 100 bases:

>100_bases
TTAAGGTGTATGCATTCACATTTGGAGCTAACCATTGATATTATCCTGAATAATGCATTTTTTTTATTTTTTAATTCAAC
ACTCGTTTTATGATGTAATG

Downstream 100 bases:

>100_bases
AATTTTGTAAGCTTAGACGTCCAATTATCGTTGTAATTGAATGTGGCATATTTAATACATTTAATTGTAGTAGTTCAATA
ATAAGTGACGTGTATTCGGA

Product: putative protein involved in tRNA-dihydrouridine synthesis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTFLPKNSFGCPYLILAPMEGVGDRSFRKAMASVGGFDEACTEFMRVPSNAHVPSLANRYEANETFPIPQAAQLMGSDPE
LMAEMARAVERRGAQRIDLNCGCPSNTVTGRGAGSSLLKEPDHLYKVAKSMVEAVNVPVTAKLRSGFTDTSLFHDNLLAA
QASGIKYLTLHPRTKVDGYGPPAKWELIAQAKQLLKIPVVGNGDILTVQDALNMLKQTKCDGLMIGRGSVINPFIFHEIK
SHFSAQPFEKKWDVFSRYIDVFTEDLKEMSVKGQLNKLKQLMSFLFKFNSKLREKKQKILTHQTVNAQSFLSFALENYQE
DWMEAN

Sequences:

>Translated_326_residues
MTFLPKNSFGCPYLILAPMEGVGDRSFRKAMASVGGFDEACTEFMRVPSNAHVPSLANRYEANETFPIPQAAQLMGSDPE
LMAEMARAVERRGAQRIDLNCGCPSNTVTGRGAGSSLLKEPDHLYKVAKSMVEAVNVPVTAKLRSGFTDTSLFHDNLLAA
QASGIKYLTLHPRTKVDGYGPPAKWELIAQAKQLLKIPVVGNGDILTVQDALNMLKQTKCDGLMIGRGSVINPFIFHEIK
SHFSAQPFEKKWDVFSRYIDVFTEDLKEMSVKGQLNKLKQLMSFLFKFNSKLREKKQKILTHQTVNAQSFLSFALENYQE
DWMEAN
>Mature_325_residues
TFLPKNSFGCPYLILAPMEGVGDRSFRKAMASVGGFDEACTEFMRVPSNAHVPSLANRYEANETFPIPQAAQLMGSDPEL
MAEMARAVERRGAQRIDLNCGCPSNTVTGRGAGSSLLKEPDHLYKVAKSMVEAVNVPVTAKLRSGFTDTSLFHDNLLAAQ
ASGIKYLTLHPRTKVDGYGPPAKWELIAQAKQLLKIPVVGNGDILTVQDALNMLKQTKCDGLMIGRGSVINPFIFHEIKS
HFSAQPFEKKWDVFSRYIDVFTEDLKEMSVKGQLNKLKQLMSFLFKFNSKLREKKQKILTHQTVNAQSFLSFALENYQED
WMEAN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=251, Percent_Identity=31.0756972111554, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI239788462, Length=251, Percent_Identity=31.0756972111554, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI31742496, Length=206, Percent_Identity=29.6116504854369, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI40807366, Length=246, Percent_Identity=30.0813008130081, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI8923374, Length=276, Percent_Identity=27.536231884058, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788462, Length=286, Percent_Identity=34.965034965035, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789660, Length=239, Percent_Identity=36.4016736401674, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693211, Length=181, Percent_Identity=30.3867403314917, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=253, Percent_Identity=30.4347826086957, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144369, Length=175, Percent_Identity=32, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=171, Percent_Identity=26.9005847953216, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=201, Percent_Identity=26.865671641791, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24585320, Length=290, Percent_Identity=28.2758620689655, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24580595, Length=296, Percent_Identity=27.7027027027027, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920448, Length=296, Percent_Identity=27.7027027027027, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921524, Length=177, Percent_Identity=31.638418079096, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45549423, Length=150, Percent_Identity=28.6666666666667, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36361; Mature: 36230

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLPKNSFGCPYLILAPMEGVGDRSFRKAMASVGGFDEACTEFMRVPSNAHVPSLANRY
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHH
EANETFPIPQAAQLMGSDPELMAEMARAVERRGAQRIDLNCGCPSNTVTGRGAGSSLLKE
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCC
PDHLYKVAKSMVEAVNVPVTAKLRSGFTDTSLFHDNLLAAQASGIKYLTLHPRTKVDGYG
HHHHHHHHHHHHHHHCCCEEEHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
PPAKWELIAQAKQLLKIPVVGNGDILTVQDALNMLKQTKCDGLMIGRGSVINPFIFHEIK
CCHHHHHHHHHHHHHCCCEECCCCEEEHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
SHFSAQPFEKKWDVFSRYIDVFTEDLKEMSVKGQLNKLKQLMSFLFKFNSKLREKKQKIL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THQTVNAQSFLSFALENYQEDWMEAN
HHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFLPKNSFGCPYLILAPMEGVGDRSFRKAMASVGGFDEACTEFMRVPSNAHVPSLANRY
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHH
EANETFPIPQAAQLMGSDPELMAEMARAVERRGAQRIDLNCGCPSNTVTGRGAGSSLLKE
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCC
PDHLYKVAKSMVEAVNVPVTAKLRSGFTDTSLFHDNLLAAQASGIKYLTLHPRTKVDGYG
HHHHHHHHHHHHHHHCCCEEEHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
PPAKWELIAQAKQLLKIPVVGNGDILTVQDALNMLKQTKCDGLMIGRGSVINPFIFHEIK
CCHHHHHHHHHHHHHCCCEECCCCEEEHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
SHFSAQPFEKKWDVFSRYIDVFTEDLKEMSVKGQLNKLKQLMSFLFKFNSKLREKKQKIL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THQTVNAQSFLSFALENYQEDWMEAN
HHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]