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The map label for this gene is ygcA [C]

Identifier: 46447178

GI number: 46447178

Start: 1849503

End: 1850831

Strand: Reverse

Name: ygcA [C]

Synonym: pc1544

Alternate gene names: 46447178

Gene position: 1850831-1849503 (Counterclockwise)

Preceding gene: 46447179

Following gene: 46447177

Centisome position: 76.66

GC content: 37.47

Gene sequence:

>1329_bases
GTGGAAGTTCCTTTTACGATTCCTGGGGATCAGGTCAATGCTATTTTATATAAAAAAACGAAAGACAAATATTTAAGCCG
TTTGTCAAAAGTAACCATGCCTTCAAATAATCGTATTGACGCGTTATGCATGCATTTTGGTTCTTGTGGAGGGTGTTGCT
GGCAACAATTGCCTTACGAGCTGCAGTTAAAGCAAAAAGAAAAATCTATTCAGAATCTTTTTAAACCCTACTTGAATGAA
AATGTAAAATGGCATTCAATTATTCCAAGTTCTCCTTGGCATTATCGGAATAAAATGGAATTGACATTTTCAAGCGATAA
AGCTGGTCGTCGCTATTTGGGATTGATTATGCAAGGAACACGCGGACATGTATTTCAAATGAAAGAATGTTTATTAGCGA
ATGGGTGGTTTGCTCAGGCTGCTAGAGCTATACAGAAATGGTGGGATAATTCAGATGTACAAGCTTACCATGCTATTAAA
GATAGAGGATCTTTAAGAACATTAACGCTACGAGAAGGCTTGAGGACAGGGGACCGCTTAGTAATGCTTACTGTATCTGG
TAATCCTGATTATGCCTTAACTAAATCCCAATTACAAAGTTTTGTTCAGGTTCTACGGGATTCTATAGAACTTTCTAATT
CTGATCAAAAGTTATCAGTTTTTCTACGAATACAACAAATTGCAAAGGGAAGACCTACCAACTTTTATGAAATGCTCCTG
TATGGTCCAGATCATATTCGAGAAAAACTCTATTTACCAGATTTCAATGAAGCTATTCAAACATTGAATTTTCGAATTAG
CCCTTCAGCATTTTTTCAACCTAATACTGAGCAAGCGGAAAAACTTTATAGCCAAGCTTTAAAGCTAGCCGATTTATCTG
CTTCTCATGTTGTTTACGATTTATATTGCGGAACAGGGACGCTGGGAATTTGCTCGGCTAAATATGTCAAAGAGGTTATT
GGAATAGAGTTAAGTCGAGAATCTGTGTTAGATGCAAGAGAAAATGTGAAAGAAAATGGTCTTTCGAATGTTACCATCAA
ATCGGGAGATGTGGGACAAGTTCTTGAAGCACTTTCGCAGGAAAACTCTCTAAAACCAGATGTTGTAATGGTAGATCCTC
CTCGGATCGGTTTAGATGCGAAAGCGATTAAACATATTTTAGACCTTAAAGCCCCTAAACTAGTTTACATTTCCTGTAAT
CCCAAAACTCAAGTGATGAATTTGGGCCCTCTCATAGATGGGGGATATCAATTGCAAATTGTCCAACCTGTTGACCAATT
CCCCCAAACTGTTCATGTGGAAAATATCACTTTGTTAACACTCCCATAA

Upstream 100 bases:

>100_bases
AATGAAACAACGTTCAATTGATGCCCAAATTAAAGAATTTTCAACCGATGGACACGGAATGGGCTCTACCCTCCTCCTAT
CAAATCAAAAAGAAATTCAA

Downstream 100 bases:

>100_bases
ATTTTTGTAAAATTGATTTTTAAATTTCTTCTATTATTAGGTTATTTCAAAAATTAATTCTTAATATTAGTATAAGTTTA
TTTCTATAATTTGGTATAAA

Product: putative rRNA methyltransferse

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MEVPFTIPGDQVNAILYKKTKDKYLSRLSKVTMPSNNRIDALCMHFGSCGGCCWQQLPYELQLKQKEKSIQNLFKPYLNE
NVKWHSIIPSSPWHYRNKMELTFSSDKAGRRYLGLIMQGTRGHVFQMKECLLANGWFAQAARAIQKWWDNSDVQAYHAIK
DRGSLRTLTLREGLRTGDRLVMLTVSGNPDYALTKSQLQSFVQVLRDSIELSNSDQKLSVFLRIQQIAKGRPTNFYEMLL
YGPDHIREKLYLPDFNEAIQTLNFRISPSAFFQPNTEQAEKLYSQALKLADLSASHVVYDLYCGTGTLGICSAKYVKEVI
GIELSRESVLDARENVKENGLSNVTIKSGDVGQVLEALSQENSLKPDVVMVDPPRIGLDAKAIKHILDLKAPKLVYISCN
PKTQVMNLGPLIDGGYQLQIVQPVDQFPQTVHVENITLLTLP

Sequences:

>Translated_442_residues
MEVPFTIPGDQVNAILYKKTKDKYLSRLSKVTMPSNNRIDALCMHFGSCGGCCWQQLPYELQLKQKEKSIQNLFKPYLNE
NVKWHSIIPSSPWHYRNKMELTFSSDKAGRRYLGLIMQGTRGHVFQMKECLLANGWFAQAARAIQKWWDNSDVQAYHAIK
DRGSLRTLTLREGLRTGDRLVMLTVSGNPDYALTKSQLQSFVQVLRDSIELSNSDQKLSVFLRIQQIAKGRPTNFYEMLL
YGPDHIREKLYLPDFNEAIQTLNFRISPSAFFQPNTEQAEKLYSQALKLADLSASHVVYDLYCGTGTLGICSAKYVKEVI
GIELSRESVLDARENVKENGLSNVTIKSGDVGQVLEALSQENSLKPDVVMVDPPRIGLDAKAIKHILDLKAPKLVYISCN
PKTQVMNLGPLIDGGYQLQIVQPVDQFPQTVHVENITLLTLP
>Mature_442_residues
MEVPFTIPGDQVNAILYKKTKDKYLSRLSKVTMPSNNRIDALCMHFGSCGGCCWQQLPYELQLKQKEKSIQNLFKPYLNE
NVKWHSIIPSSPWHYRNKMELTFSSDKAGRRYLGLIMQGTRGHVFQMKECLLANGWFAQAARAIQKWWDNSDVQAYHAIK
DRGSLRTLTLREGLRTGDRLVMLTVSGNPDYALTKSQLQSFVQVLRDSIELSNSDQKLSVFLRIQQIAKGRPTNFYEMLL
YGPDHIREKLYLPDFNEAIQTLNFRISPSAFFQPNTEQAEKLYSQALKLADLSASHVVYDLYCGTGTLGICSAKYVKEVI
GIELSRESVLDARENVKENGLSNVTIKSGDVGQVLEALSQENSLKPDVVMVDPPRIGLDAKAIKHILDLKAPKLVYISCN
PKTQVMNLGPLIDGGYQLQIVQPVDQFPQTVHVENITLLTLP

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family

Homologues:

Organism=Homo sapiens, GI34222389, Length=196, Percent_Identity=40.3061224489796, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI51173878, Length=196, Percent_Identity=40.3061224489796, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI269784680, Length=377, Percent_Identity=28.3819628647215, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI269784676, Length=377, Percent_Identity=28.3819628647215, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI20149688, Length=377, Percent_Identity=28.3819628647215, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI269784678, Length=216, Percent_Identity=34.7222222222222, Blast_Score=109, Evalue=7e-24,
Organism=Escherichia coli, GI1787083, Length=396, Percent_Identity=26.010101010101, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789148, Length=448, Percent_Identity=24.5535714285714, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1790403, Length=208, Percent_Identity=29.8076923076923, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71997438, Length=164, Percent_Identity=31.0975609756098, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=431, Percent_Identity=26.4501160092807, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24666579, Length=421, Percent_Identity=28.7410926365796, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1544_PARUW (Q6MAY1)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008543.1
- ProteinModelPortal:   Q6MAY1
- SMR:   Q6MAY1
- STRING:   Q6MAY1
- GeneID:   2781040
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1544
- NMPDR:   fig|264201.1.peg.1544
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   LNGLNNC
- PhylomeDB:   Q6MAY1
- ProtClustDB:   CLSK2762417
- BioCyc:   CPRO264201:PC1544-MONOMER
- InterPro:   IPR001566
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: 2.1.1.- [C]

Molecular weight: Translated: 49972; Mature: 49972

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 399-399 BINDING 273-273 BINDING 302-302 BINDING 323-323 BINDING 372-372

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVPFTIPGDQVNAILYKKTKDKYLSRLSKVTMPSNNRIDALCMHFGSCGGCCWQQLPYE
CCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHCCHH
LQLKQKEKSIQNLFKPYLNENVKWHSIIPSSPWHYRNKMELTFSSDKAGRRYLGLIMQGT
EEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEEEECC
RGHVFQMKECLLANGWFAQAARAIQKWWDNSDVQAYHAIKDRGSLRTLTLREGLRTGDRL
CCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEHHHHHCCCCEEEEEHHHCCCCCCEE
VMLTVSGNPDYALTKSQLQSFVQVLRDSIELSNSDQKLSVFLRIQQIAKGRPTNFYEMLL
EEEEECCCCCCEECHHHHHHHHHHHHHHHHCCCCCCHHHHHEEHHHHHCCCCCCEEEEEE
YGPDHIREKLYLPDFNEAIQTLNFRISPSAFFQPNTEQAEKLYSQALKLADLSASHVVYD
ECCHHHHHCCCCCCHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
LYCGTGTLGICSAKYVKEVIGIELSRESVLDARENVKENGLSNVTIKSGDVGQVLEALSQ
EEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
ENSLKPDVVMVDPPRIGLDAKAIKHILDLKAPKLVYISCNPKTQVMNLGPLIDGGYQLQI
CCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEECCCEECCCEEEEE
VQPVDQFPQTVHVENITLLTLP
ECCHHHCCCEEEECCEEEEECC
>Mature Secondary Structure
MEVPFTIPGDQVNAILYKKTKDKYLSRLSKVTMPSNNRIDALCMHFGSCGGCCWQQLPYE
CCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHCCHH
LQLKQKEKSIQNLFKPYLNENVKWHSIIPSSPWHYRNKMELTFSSDKAGRRYLGLIMQGT
EEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEEEECC
RGHVFQMKECLLANGWFAQAARAIQKWWDNSDVQAYHAIKDRGSLRTLTLREGLRTGDRL
CCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEHHHHHCCCCEEEEEHHHCCCCCCEE
VMLTVSGNPDYALTKSQLQSFVQVLRDSIELSNSDQKLSVFLRIQQIAKGRPTNFYEMLL
EEEEECCCCCCEECHHHHHHHHHHHHHHHHCCCCCCHHHHHEEHHHHHCCCCCCEEEEEE
YGPDHIREKLYLPDFNEAIQTLNFRISPSAFFQPNTEQAEKLYSQALKLADLSASHVVYD
ECCHHHHHCCCCCCHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
LYCGTGTLGICSAKYVKEVIGIELSRESVLDARENVKENGLSNVTIKSGDVGQVLEALSQ
EEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
ENSLKPDVVMVDPPRIGLDAKAIKHILDLKAPKLVYISCNPKTQVMNLGPLIDGGYQLQI
CCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEECCCEECCCEEEEE
VQPVDQFPQTVHVENITLLTLP
ECCHHHCCCEEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA