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The map label for this gene is ykfC [H]

Identifier: 46447135

GI number: 46447135

Start: 1789140

End: 1789943

Strand: Reverse

Name: ykfC [H]

Synonym: pc1501

Alternate gene names: 46447135

Gene position: 1789943-1789140 (Counterclockwise)

Preceding gene: 46447145

Following gene: 46447125

Centisome position: 74.13

GC content: 35.07

Gene sequence:

>804_bases
ATGTATGAGATGAACATTAATTATCCAGTTGCAAATTTATATCGATTACCAAATGAACACGTCGAGGTAATTTCGCAAGC
CATTTATGGTTGGAAAGTTAAGATTATCAAGAAAGATGACAAATTTTATTTAGTCGAAACGGTCGATGGTTACCAAGGAT
GGATCAATCCAATACAGATGATTGAGAGCAATACGATATCTTCTTTTCCGCTTATTAAAATTACATCGAATGCAGCACAT
ATTTATAAGTCTCCTCATGTAAATCGAGAAAAGCCTTTTTTAACCCTTCCTTTTGAAGTAGAATTACCTCTACTAGAAAC
ACTTGAAGAAGAAGAAGGAAGATGGTTACAAATCCAACTCATGAATGGGGAAAAAGGATGGATTCAAAAAGGTGATATCA
CACTGGACCTTTTTTCAACTAATTTAGAAAAAGTGGTAGATGGTAGCCAGCAGTTTTTAAACTTGCCTTACACGTGGGGG
GGAGTTTCGAGTTTTGGTTATGATTGCTCAGGTTTTATTCAAATGATATTTAGACAGGTGAAAATCATTCTTCCTCGAGA
TGCCTCTCAACAAATTACCTTTCCCCTTTTTCAGTTTATCGATTGGAATAATAAAGAAAGGGGAGATGTGATTTTTTTTG
GTTCTAATGATGACTCCATTAAACATGTGGGACTTTATTTAGGGAATGATCAATTGATTCATGCTTCTGTAAAACCCAAA
CCGACTTTGCAAATTTCTTCTTTAGAAGAGCCTTCCCTCAAAAATCGTTTTGGTTATCGTACTGTTCGTAGGCTTAAGAT
TTAA

Upstream 100 bases:

>100_bases
TTTGAAAATTAATTTTATAATTTTCAATCCAAGTTTATATTTATACGAAATTATTTGATTTATAAAAAGTAAAGTTATCA
ACAGAAAGTGATTATATACT

Downstream 100 bases:

>100_bases
AGGAAAAACAAAGGTATTCAATCCATATTAATTGATTGAAAGAGTGTGAAAGATCAAGGATAAACTTATATCAAGTCAGT
TAATGCAAATAATTCTATCC

Product: hypothetical protein

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MYEMNINYPVANLYRLPNEHVEVISQAIYGWKVKIIKKDDKFYLVETVDGYQGWINPIQMIESNTISSFPLIKITSNAAH
IYKSPHVNREKPFLTLPFEVELPLLETLEEEEGRWLQIQLMNGEKGWIQKGDITLDLFSTNLEKVVDGSQQFLNLPYTWG
GVSSFGYDCSGFIQMIFRQVKIILPRDASQQITFPLFQFIDWNNKERGDVIFFGSNDDSIKHVGLYLGNDQLIHASVKPK
PTLQISSLEEPSLKNRFGYRTVRRLKI

Sequences:

>Translated_267_residues
MYEMNINYPVANLYRLPNEHVEVISQAIYGWKVKIIKKDDKFYLVETVDGYQGWINPIQMIESNTISSFPLIKITSNAAH
IYKSPHVNREKPFLTLPFEVELPLLETLEEEEGRWLQIQLMNGEKGWIQKGDITLDLFSTNLEKVVDGSQQFLNLPYTWG
GVSSFGYDCSGFIQMIFRQVKIILPRDASQQITFPLFQFIDWNNKERGDVIFFGSNDDSIKHVGLYLGNDQLIHASVKPK
PTLQISSLEEPSLKNRFGYRTVRRLKI
>Mature_267_residues
MYEMNINYPVANLYRLPNEHVEVISQAIYGWKVKIIKKDDKFYLVETVDGYQGWINPIQMIESNTISSFPLIKITSNAAH
IYKSPHVNREKPFLTLPFEVELPLLETLEEEEGRWLQIQLMNGEKGWIQKGDITLDLFSTNLEKVVDGSQQFLNLPYTWG
GVSSFGYDCSGFIQMIFRQVKIILPRDASQQITFPLFQFIDWNNKERGDVIFFGSNDDSIKHVGLYLGNDQLIHASVKPK
PTLQISSLEEPSLKNRFGYRTVRRLKI

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1788501, Length=123, Percent_Identity=34.1463414634146, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786421, Length=156, Percent_Identity=29.4871794871795, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 30926; Mature: 30926

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEMNINYPVANLYRLPNEHVEVISQAIYGWKVKIIKKDDKFYLVETVDGYQGWINPIQM
CEEEEECCCHHHHHHCCHHHHHHHHHHHCCEEEEEEEECCCEEEEEECCCCCHHCCHHHH
IESNTISSFPLIKITSNAAHIYKSPHVNREKPFLTLPFEVELPLLETLEEEEGRWLQIQL
HHCCCCCCCEEEEEECCCEEEECCCCCCCCCCEEEECEEECCCHHHHHHHCCCCEEEEEE
MNGEKGWIQKGDITLDLFSTNLEKVVDGSQQFLNLPYTWGGVSSFGYDCSGFIQMIFRQV
ECCCCCCEEECCEEEEEECCCHHHHHCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHH
KIILPRDASQQITFPLFQFIDWNNKERGDVIFFGSNDDSIKHVGLYLGNDQLIHASVKPK
EEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEEECCCEEEEEECCCC
PTLQISSLEEPSLKNRFGYRTVRRLKI
CCEEECCCCCCCHHHHCCHHHHHEECC
>Mature Secondary Structure
MYEMNINYPVANLYRLPNEHVEVISQAIYGWKVKIIKKDDKFYLVETVDGYQGWINPIQM
CEEEEECCCHHHHHHCCHHHHHHHHHHHCCEEEEEEEECCCEEEEEECCCCCHHCCHHHH
IESNTISSFPLIKITSNAAHIYKSPHVNREKPFLTLPFEVELPLLETLEEEEGRWLQIQL
HHCCCCCCCEEEEEECCCEEEECCCCCCCCCCEEEECEEECCCHHHHHHHCCCCEEEEEE
MNGEKGWIQKGDITLDLFSTNLEKVVDGSQQFLNLPYTWGGVSSFGYDCSGFIQMIFRQV
ECCCCCCEEECCEEEEEECCCHHHHHCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHH
KIILPRDASQQITFPLFQFIDWNNKERGDVIFFGSNDDSIKHVGLYLGNDQLIHASVKPK
EEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEEECCCEEEEEECCCC
PTLQISSLEEPSLKNRFGYRTVRRLKI
CCEEECCCCCCCHHHHCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]