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The map label for this gene is ald [H]

Identifier: 46447120

GI number: 46447120

Start: 1766062

End: 1767174

Strand: Reverse

Name: ald [H]

Synonym: pc1486

Alternate gene names: 46447120

Gene position: 1767174-1766062 (Counterclockwise)

Preceding gene: 46447121

Following gene: 46447119

Centisome position: 73.19

GC content: 40.16

Gene sequence:

>1113_bases
ATGATTATTGGAATTCCGAAAGAGATTAAAAATCATGAATATCGTGTCGGAGCTACTCCTAGCATGGTGCGTTTATTAGT
AGATGCAGGCCATCAAGTATTTGTGCAATCCCTTGCTGGAGATAAAATTGGATATAATGATGAAATGTTCACCCGTGCAG
GCGCCAAAATTGTTCAGTCGGCAGAAGAGGTTTATAATTCTGAAATGATTATTAAAGTTAAGGAACCCCAACTTAGTGAA
TTTCCTCTTTTGAAAGAGGGGCAAATTTTATTTTGCTATTTACATTTAGCTCCAGATCCTGAGCAAACTAAACATCTGGT
AGAAAAAAAAGTTGTTGCTATTGCTTATGAAACTGTTACTGATGTTCAAGGGCGTTTACCTCTTCTTATTCCTATGAGTG
AAATTGCAGGTCGCATCGCTATTCAAGTGGGAGCTACAAGCTTACAACTCAATTACGGAGGGCAAGGGTTATTATTAGGT
GGTGTGCCTGGTGTTGCACCTGCTGAGGTTGTCGTTATTGGGGGTGGCGTTGTTGGAACAGAAGCAGCCCGTATGGCTCT
TGGGCTTGGAGCAAGTGTGACAATATTTGACAGAGATTTAAACCGTTTACGGTTTTTGGATGCACTATATGGACCTACTT
TGAAGACAATGTATTCTTCTCCTTTAACAATTGAGGAAGCGGTTTCTAAGGCAGCTCTCGTCATTGGTGCTGTCCTTATT
CCAGGTAAAACAGCTCCTAAGCTTGTAACGAGGAAAATGATCCAAAAAATGTCTCCCGGTACAGTAGTTGTCGATGTAGC
AATAGATCAAGGTGGATGTATGGAAACGTCAAGACCTACCACTCACACTGAACCTACTTATGTAGTTGATGGTGTTCTTC
ATTATTGTGTAACAAATATGCCGGGCACTTGTGCAAGAACATCAACTCAAGCATTAACAAATGCTACTACAGAATATGCT
CTTGTGATTGCCAATAAAGGATGGAAAAAAGCACTACTCGATCATGTGGGTTTACGTCATGGATTGAATGTTTGTTTTGG
GCAAATTACGAATGAGTCAGTGGCTTACGATTTAGGTTATACATATGTTCCAGCCGAAAATCTCTTATCTTAA

Upstream 100 bases:

>100_bases
AAAGGTGTTTCTAAATTGTTGTCATTAGAATTTACTCATCAAACTCCAGAAGTGACTTTAGTAGATTAATCAAACTGTTA
TTATAAATTGAGAGAGAATT

Downstream 100 bases:

>100_bases
ATTTTTCAAATAAAAAACGGTTAACAGGTTGCAAAACACCTCATTTTGAGTTTTGATACATGTTTTTCTTTCATTAAATT
CAAATTTAATTTTCTGAGGA

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MIIGIPKEIKNHEYRVGATPSMVRLLVDAGHQVFVQSLAGDKIGYNDEMFTRAGAKIVQSAEEVYNSEMIIKVKEPQLSE
FPLLKEGQILFCYLHLAPDPEQTKHLVEKKVVAIAYETVTDVQGRLPLLIPMSEIAGRIAIQVGATSLQLNYGGQGLLLG
GVPGVAPAEVVVIGGGVVGTEAARMALGLGASVTIFDRDLNRLRFLDALYGPTLKTMYSSPLTIEEAVSKAALVIGAVLI
PGKTAPKLVTRKMIQKMSPGTVVVDVAIDQGGCMETSRPTTHTEPTYVVDGVLHYCVTNMPGTCARTSTQALTNATTEYA
LVIANKGWKKALLDHVGLRHGLNVCFGQITNESVAYDLGYTYVPAENLLS

Sequences:

>Translated_370_residues
MIIGIPKEIKNHEYRVGATPSMVRLLVDAGHQVFVQSLAGDKIGYNDEMFTRAGAKIVQSAEEVYNSEMIIKVKEPQLSE
FPLLKEGQILFCYLHLAPDPEQTKHLVEKKVVAIAYETVTDVQGRLPLLIPMSEIAGRIAIQVGATSLQLNYGGQGLLLG
GVPGVAPAEVVVIGGGVVGTEAARMALGLGASVTIFDRDLNRLRFLDALYGPTLKTMYSSPLTIEEAVSKAALVIGAVLI
PGKTAPKLVTRKMIQKMSPGTVVVDVAIDQGGCMETSRPTTHTEPTYVVDGVLHYCVTNMPGTCARTSTQALTNATTEYA
LVIANKGWKKALLDHVGLRHGLNVCFGQITNESVAYDLGYTYVPAENLLS
>Mature_370_residues
MIIGIPKEIKNHEYRVGATPSMVRLLVDAGHQVFVQSLAGDKIGYNDEMFTRAGAKIVQSAEEVYNSEMIIKVKEPQLSE
FPLLKEGQILFCYLHLAPDPEQTKHLVEKKVVAIAYETVTDVQGRLPLLIPMSEIAGRIAIQVGATSLQLNYGGQGLLLG
GVPGVAPAEVVVIGGGVVGTEAARMALGLGASVTIFDRDLNRLRFLDALYGPTLKTMYSSPLTIEEAVSKAALVIGAVLI
PGKTAPKLVTRKMIQKMSPGTVVVDVAIDQGGCMETSRPTTHTEPTYVVDGVLHYCVTNMPGTCARTSTQALTNATTEYA
LVIANKGWKKALLDHVGLRHGLNVCFGQITNESVAYDLGYTYVPAENLLS

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=342, Percent_Identity=32.1637426900585, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI122939153, Length=342, Percent_Identity=32.1637426900585, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1787887, Length=332, Percent_Identity=34.9397590361446, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17550456, Length=342, Percent_Identity=30.9941520467836, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39721; Mature: 39721

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGIPKEIKNHEYRVGATPSMVRLLVDAGHQVFVQSLAGDKIGYNDEMFTRAGAKIVQS
CEECCCHHHCCCCEEECCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AEEVYNSEMIIKVKEPQLSEFPLLKEGQILFCYLHLAPDPEQTKHLVEKKVVAIAYETVT
HHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHEEHHHHH
DVQGRLPLLIPMSEIAGRIAIQVGATSLQLNYGGQGLLLGGVPGVAPAEVVVIGGGVVGT
CCCCCCEEEEEHHHHCCEEEEEECCEEEEEEECCCEEEEECCCCCCCCEEEEECCCEECH
EAARMALGLGASVTIFDRDLNRLRFLDALYGPTLKTMYSSPLTIEEAVSKAALVIGAVLI
HHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHEEE
PGKTAPKLVTRKMIQKMSPGTVVVDVAIDQGGCMETSRPTTHTEPTYVVDGVLHYCVTNM
CCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
PGTCARTSTQALTNATTEYALVIANKGWKKALLDHVGLRHGLNVCFGQITNESVAYDLGY
CCCCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCE
TYVPAENLLS
EEECHHHHCC
>Mature Secondary Structure
MIIGIPKEIKNHEYRVGATPSMVRLLVDAGHQVFVQSLAGDKIGYNDEMFTRAGAKIVQS
CEECCCHHHCCCCEEECCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AEEVYNSEMIIKVKEPQLSEFPLLKEGQILFCYLHLAPDPEQTKHLVEKKVVAIAYETVT
HHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHEEHHHHH
DVQGRLPLLIPMSEIAGRIAIQVGATSLQLNYGGQGLLLGGVPGVAPAEVVVIGGGVVGT
CCCCCCEEEEEHHHHCCEEEEEECCEEEEEEECCCEEEEECCCCCCCCEEEEECCCEECH
EAARMALGLGASVTIFDRDLNRLRFLDALYGPTLKTMYSSPLTIEEAVSKAALVIGAVLI
HHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHEEE
PGKTAPKLVTRKMIQKMSPGTVVVDVAIDQGGCMETSRPTTHTEPTYVVDGVLHYCVTNM
CCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
PGTCARTSTQALTNATTEYALVIANKGWKKALLDHVGLRHGLNVCFGQITNESVAYDLGY
CCCCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCE
TYVPAENLLS
EEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]