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The map label for this gene is prfB [H]

Identifier: 46446965

GI number: 46446965

Start: 1586897

End: 1587877

Strand: Reverse

Name: prfB [H]

Synonym: pc1331

Alternate gene names: 46446965

Gene position: 1587877-1586897 (Counterclockwise)

Preceding gene: 46446971

Following gene: 46446964

Centisome position: 65.77

GC content: 37.92

Gene sequence:

>981_bases
ATGGGAGAAAGCTCGTTTTGGAATAATTCTGAACAAGCGCAATTAATTATTAATGAATGCCGCGATTTGCGAGCTTGGAC
TGTCCCTTATCAGGAACTTAAAACAAAATTTGAAAATTGCAAAGAGTTACTTCCTGAAGCCTATGAATTAAATGAAGAAT
CTTTTATTGAAGAACTTTTAAGAGATTTAGATTCTATTGAGAAAGTGCTGGGAGATTTAGAAGTTCAGCGAATGTTATCT
GGAGAAATGGATAACAAAGATTGCTATTTGACGATTAATTCAGGAGCTGGGGGAACAGAAGCTTGTGATTGGGCTCAGAT
GTTGTCGAGGATGTACCAACGCTGGGCGGCTAAGAAGGGATGGAAAATTGAAATTATCGATTTTGTCGATGGAGACGTGG
TTGGTTTAAAAAGTATTACTCTGCGTTTTCAAGGCTCTTATGCTTATGGTTATAGCAAAGCTGAAAAAGGCGTACATCGT
TTAGTGCGCATTTCCCCTTTCGACAGTAATGCTAAGAGGCATACCAGTTTTGCATCAGTGGATGTTTCTCCAGAAATAAC
TACTGACATTCAAGTTGAAATTAAACCTGAGGATGTTAGAGTTGATACTTTTAGATCTTCAGGAGCTGGAGGGCAGCACG
TAAACAAAACAGATTCTGCTGTACGTTTAACCCACATGCCTACAGGAATTGTTGTTTCTTGTCAAACTCAACGAAGTCAA
GTACAAAATAAAGAAGCTTGTTATCAGATGCTTCGTTCAAAGTTATATGAAATGGAAGTTTTAGAGCGTGAAAAAGCCCT
AAATAACCTAAAAGGGGAAAAAAAAGAAAACGCTTGGGGTAGCCAAATCCGCAATTATGTTTTTCAACCTTATACGCTTG
TTAAAGACACAAGGACGAAAGCTGAAAGTGGTAACATACAAGCCGTAATGGATGGTGAATTAGATGAATTTGTTAACGCT
TATTTAAAGGAGTTTGGCTGA

Upstream 100 bases:

>100_bases
GAATTGAAAGTAAAAGGAATCGAAGAACGTCTTCTTCACATGTGGAGGTATCTTTGACTTGGCTGCGAAGGAAGCTAGGG
TAAAAGAATTAGAGACATTA

Downstream 100 bases:

>100_bases
TGAATAGTTCAAAGGCGATTCCTACTGGGATTGAAGTGCGCTATACGGAATCTGGAGATGCTAAGTACCTTAAAGAATGG
TTAATGGATCCAGAAATTCG

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MGESSFWNNSEQAQLIINECRDLRAWTVPYQELKTKFENCKELLPEAYELNEESFIEELLRDLDSIEKVLGDLEVQRMLS
GEMDNKDCYLTINSGAGGTEACDWAQMLSRMYQRWAAKKGWKIEIIDFVDGDVVGLKSITLRFQGSYAYGYSKAEKGVHR
LVRISPFDSNAKRHTSFASVDVSPEITTDIQVEIKPEDVRVDTFRSSGAGGQHVNKTDSAVRLTHMPTGIVVSCQTQRSQ
VQNKEACYQMLRSKLYEMEVLEREKALNNLKGEKKENAWGSQIRNYVFQPYTLVKDTRTKAESGNIQAVMDGELDEFVNA
YLKEFG

Sequences:

>Translated_326_residues
MGESSFWNNSEQAQLIINECRDLRAWTVPYQELKTKFENCKELLPEAYELNEESFIEELLRDLDSIEKVLGDLEVQRMLS
GEMDNKDCYLTINSGAGGTEACDWAQMLSRMYQRWAAKKGWKIEIIDFVDGDVVGLKSITLRFQGSYAYGYSKAEKGVHR
LVRISPFDSNAKRHTSFASVDVSPEITTDIQVEIKPEDVRVDTFRSSGAGGQHVNKTDSAVRLTHMPTGIVVSCQTQRSQ
VQNKEACYQMLRSKLYEMEVLEREKALNNLKGEKKENAWGSQIRNYVFQPYTLVKDTRTKAESGNIQAVMDGELDEFVNA
YLKEFG
>Mature_325_residues
GESSFWNNSEQAQLIINECRDLRAWTVPYQELKTKFENCKELLPEAYELNEESFIEELLRDLDSIEKVLGDLEVQRMLSG
EMDNKDCYLTINSGAGGTEACDWAQMLSRMYQRWAAKKGWKIEIIDFVDGDVVGLKSITLRFQGSYAYGYSKAEKGVHRL
VRISPFDSNAKRHTSFASVDVSPEITTDIQVEIKPEDVRVDTFRSSGAGGQHVNKTDSAVRLTHMPTGIVVSCQTQRSQV
QNKEACYQMLRSKLYEMEVLEREKALNNLKGEKKENAWGSQIRNYVFQPYTLVKDTRTKAESGNIQAVMDGELDEFVNAY
LKEFG

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=250, Percent_Identity=40, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI34577120, Length=323, Percent_Identity=34.3653250773994, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=42.2818791946309, Blast_Score=127, Evalue=9e-30,
Organism=Escherichia coli, GI2367172, Length=317, Percent_Identity=49.211356466877, Blast_Score=327, Evalue=6e-91,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=38.8663967611336, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=266, Percent_Identity=33.8345864661654, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=33.6477987421384, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19921226, Length=208, Percent_Identity=39.4230769230769, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37182; Mature: 37051

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGESSFWNNSEQAQLIINECRDLRAWTVPYQELKTKFENCKELLPEAYELNEESFIEELL
CCCCCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHH
RDLDSIEKVLGDLEVQRMLSGEMDNKDCYLTINSGAGGTEACDWAQMLSRMYQRWAAKKG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCC
WKIEIIDFVDGDVVGLKSITLRFQGSYAYGYSKAEKGVHRLVRISPFDSNAKRHTSFASV
CEEEEEEECCCCEEEEEEEEEEEECCEEECHHHHHHHHHHHEEECCCCCCCHHCCCEEEE
DVSPEITTDIQVEIKPEDVRVDTFRSSGAGGQHVNKTDSAVRLTHMPTGIVVSCQTQRSQ
ECCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECHHHHH
VQNKEACYQMLRSKLYEMEVLEREKALNNLKGEKKENAWGSQIRNYVFQPYTLVKDTRTK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
AESGNIQAVMDGELDEFVNAYLKEFG
CCCCCEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GESSFWNNSEQAQLIINECRDLRAWTVPYQELKTKFENCKELLPEAYELNEESFIEELL
CCCCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHH
RDLDSIEKVLGDLEVQRMLSGEMDNKDCYLTINSGAGGTEACDWAQMLSRMYQRWAAKKG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCC
WKIEIIDFVDGDVVGLKSITLRFQGSYAYGYSKAEKGVHRLVRISPFDSNAKRHTSFASV
CEEEEEEECCCCEEEEEEEEEEEECCEEECHHHHHHHHHHHEEECCCCCCCHHCCCEEEE
DVSPEITTDIQVEIKPEDVRVDTFRSSGAGGQHVNKTDSAVRLTHMPTGIVVSCQTQRSQ
ECCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECHHHHH
VQNKEACYQMLRSKLYEMEVLEREKALNNLKGEKKENAWGSQIRNYVFQPYTLVKDTRTK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
AESGNIQAVMDGELDEFVNAYLKEFG
CCCCCEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]