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The map label for this gene is miaA

Identifier: 46446877

GI number: 46446877

Start: 1481691

End: 1482725

Strand: Reverse

Name: miaA

Synonym: pc1243

Alternate gene names: 46446877

Gene position: 1482725-1481691 (Counterclockwise)

Preceding gene: 46446878

Following gene: 46446876

Centisome position: 61.41

GC content: 35.27

Gene sequence:

>1035_bases
GTGATTGGCAACTTTTCTTGTGAAACTGATGAAATCAAACGTATTGTTTTAAATTTTGCATTACAAGTTCAGAAAAAATT
TCCTTCTAATTTTCAAAAAAATAAGAAAAGAATAATAGTTATTGCAGGACCTACATGTTGTGGAAAATCAGCTTTAGCAT
TGAATTTAGCTCAAACTATGGATGGAGAAATCATCTCAGCTGATTCGATGCAAGTTTATCGAGGGATGGATATTGGGACG
GCTAAGGCAACTAAAGAAGAACGACTTTTTGTTCCCCATCATCTAATAGATATTAGAGATATTCAAGAAAGTTTTAATGT
CGTAGATTTTTATTATGAAGCTAGACAGGCTTGTCAGAAAATTTTAGACCAAGGGAATGTTCCGATTATTGCTGGAGGGT
CGGGCTTTTATTTACACGCTCTTTTATATGGTCCCCCCAGTGGCCCTCCTTCAGTTCCTGAGGTAAGAAAATCGTTTGAA
GATGAAATTGAACGATTGGGATCAGAAATACTTTATGAAAGGCTTTCTCAATTAGATCCTCAATATGCTAAAACAATCAC
AAAGAATGATAAACAAAAAATTGTGAGAGCTTTAGAAATTATGATGCTAACCAACAAAAAGGTTAGCAAGTTATCTTGGA
AAGGTAGAAGGAAACCCCAAAACTATGATTTTAGATGTTGGTTTCTTCATCGACCAAAAGAAAAATTATATGAACGTATT
GATAAACGTTGCGATAAGATGCTTGAGGAAGGGTTTATGGATGAAGTGCGACACCTAGATTCTTTAGGTATTCGAGGTAA
CTCATCAGCTTCACAGGCAATTGGTTATAGACAAGCGCTTAATTTTCTAAAGACTGAGCAAACAGCATCACAATACCAAG
AATTTATTCGATCTTTCAAGCAAGCGACAAGGCATTATGCTAAACGTCAATTTACATGGTTTCGAAAAGAACCATTGTTT
CGTTGGTTGGATGTCGATATGCATGATCCAGAAGTTGTTTTTGATATGATCCTGAAAGACTATGAACTGCTTTAA

Upstream 100 bases:

>100_bases
ATTTAAATTTTCATTTGTTAAATAAAAATTTAATTACAATTTTATTCGATACTGAATTGCTGTCGAAGTTTCAAAACAGA
CTAATCGTTGGATGGGTGAT

Downstream 100 bases:

>100_bases
TTTATTTTAAAGGCAAAAAGGTAATCTAATTTTTGTTTTCATTTGTATGAGTTGGTATCTCTGATTCAATAACTCTTATC
AATCATTTAAAGTTTGAAGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIGNFSCETDEIKRIVLNFALQVQKKFPSNFQKNKKRIIVIAGPTCCGKSALALNLAQTMDGEIISADSMQVYRGMDIGT
AKATKEERLFVPHHLIDIRDIQESFNVVDFYYEARQACQKILDQGNVPIIAGGSGFYLHALLYGPPSGPPSVPEVRKSFE
DEIERLGSEILYERLSQLDPQYAKTITKNDKQKIVRALEIMMLTNKKVSKLSWKGRRKPQNYDFRCWFLHRPKEKLYERI
DKRCDKMLEEGFMDEVRHLDSLGIRGNSSASQAIGYRQALNFLKTEQTASQYQEFIRSFKQATRHYAKRQFTWFRKEPLF
RWLDVDMHDPEVVFDMILKDYELL

Sequences:

>Translated_344_residues
MIGNFSCETDEIKRIVLNFALQVQKKFPSNFQKNKKRIIVIAGPTCCGKSALALNLAQTMDGEIISADSMQVYRGMDIGT
AKATKEERLFVPHHLIDIRDIQESFNVVDFYYEARQACQKILDQGNVPIIAGGSGFYLHALLYGPPSGPPSVPEVRKSFE
DEIERLGSEILYERLSQLDPQYAKTITKNDKQKIVRALEIMMLTNKKVSKLSWKGRRKPQNYDFRCWFLHRPKEKLYERI
DKRCDKMLEEGFMDEVRHLDSLGIRGNSSASQAIGYRQALNFLKTEQTASQYQEFIRSFKQATRHYAKRQFTWFRKEPLF
RWLDVDMHDPEVVFDMILKDYELL
>Mature_344_residues
MIGNFSCETDEIKRIVLNFALQVQKKFPSNFQKNKKRIIVIAGPTCCGKSALALNLAQTMDGEIISADSMQVYRGMDIGT
AKATKEERLFVPHHLIDIRDIQESFNVVDFYYEARQACQKILDQGNVPIIAGGSGFYLHALLYGPPSGPPSVPEVRKSFE
DEIERLGSEILYERLSQLDPQYAKTITKNDKQKIVRALEIMMLTNKKVSKLSWKGRRKPQNYDFRCWFLHRPKEKLYERI
DKRCDKMLEEGFMDEVRHLDSLGIRGNSSASQAIGYRQALNFLKTEQTASQYQEFIRSFKQATRHYAKRQFTWFRKEPLF
RWLDVDMHDPEVVFDMILKDYELL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=32.0512820512821, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=37.2549019607843, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=310, Percent_Identity=27.4193548387097, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=290, Percent_Identity=32.7586206896552, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24649830, Length=330, Percent_Identity=29.3939393939394, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PARUW (Q6MBT2)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008242.1
- ProteinModelPortal:   Q6MBT2
- SMR:   Q6MBT2
- STRING:   Q6MBT2
- GeneID:   2781014
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1243
- NMPDR:   fig|264201.1.peg.1243
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- PhylomeDB:   Q6MBT2
- ProtClustDB:   PRK00091
- BioCyc:   CPRO264201:PC1243-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 40058; Mature: 40058

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGNFSCETDEIKRIVLNFALQVQKKFPSNFQKNKKRIIVIAGPTCCGKSALALNLAQTM
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHC
DGEIISADSMQVYRGMDIGTAKATKEERLFVPHHLIDIRDIQESFNVVDFYYEARQACQK
CCCEECCCCHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILDQGNVPIIAGGSGFYLHALLYGPPSGPPSVPEVRKSFEDEIERLGSEILYERLSQLDP
HHHCCCCEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QYAKTITKNDKQKIVRALEIMMLTNKKVSKLSWKGRRKPQNYDFRCWFLHRPKEKLYERI
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHH
DKRCDKMLEEGFMDEVRHLDSLGIRGNSSASQAIGYRQALNFLKTEQTASQYQEFIRSFK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QATRHYAKRQFTWFRKEPLFRWLDVDMHDPEVVFDMILKDYELL
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGNFSCETDEIKRIVLNFALQVQKKFPSNFQKNKKRIIVIAGPTCCGKSALALNLAQTM
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHC
DGEIISADSMQVYRGMDIGTAKATKEERLFVPHHLIDIRDIQESFNVVDFYYEARQACQK
CCCEECCCCHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILDQGNVPIIAGGSGFYLHALLYGPPSGPPSVPEVRKSFEDEIERLGSEILYERLSQLDP
HHHCCCCEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QYAKTITKNDKQKIVRALEIMMLTNKKVSKLSWKGRRKPQNYDFRCWFLHRPKEKLYERI
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHH
DKRCDKMLEEGFMDEVRHLDSLGIRGNSSASQAIGYRQALNFLKTEQTASQYQEFIRSFK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QATRHYAKRQFTWFRKEPLFRWLDVDMHDPEVVFDMILKDYELL
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA