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The map label for this gene is tpiA

Identifier: 46446435

GI number: 46446435

Start: 980629

End: 981423

Strand: Direct

Name: tpiA

Synonym: pc0801

Alternate gene names: 46446435

Gene position: 980629-981423 (Clockwise)

Preceding gene: 46446433

Following gene: 46446436

Centisome position: 40.61

GC content: 38.36

Gene sequence:

>795_bases
ATGAGTCCATCGCATCGCCCTGTTGTCATTACAGGCAATTGGAAAATGTATAAAACCGTTAAAGAGGCTTGCACTTTTGC
AAAAGCCTTACTTCCAGTGGTTGAAGTAAGCCCTATTCAAGTATGGATTGCTGTTCCTTTTACAGCCATTTATCCTGTTA
AACAAGAGATTCGAAATTCGCGATTAGTAATTGGTGCGCAAAATATGAACGATGCCTCTGAAGGGGCTTTTACAGGCGAA
ATCGCAGGTAAAATGGTTAAAGAAGCAGGTGCTTCCTTTGTTTTATTAGGGCACTCTGAACGTCGCCATCTTTATAAAGA
AGATAATGCTTTTATTAATCGAAAAGTGAAGAAAGCTTTAGAAATTGGCCTCACTCCTGTGCTTTGTGTTGGTGAAACAT
TCGAAGAACGAAAATCCGGTGAAACACAACAAATCATTCGTACTCAAATACAAGAATGTTTAGCTGGATTAACAGCTGAA
GACTTAAAGACCTTAATAATTGCTTACGAACCTGTTTGGGCTATTGGGAATGGTCAAAACGCAAAACCTGAAGGGGCACA
AGAAATTCATCAGTTTTGTCGAAAAATCATTAAAGAAATTTTTTCTGAAGAGTTAGCCGAACAGATTGTCATTCAATATG
GCGGATCGGTGAATCCTTCTAACGCAATCTCATTACTTAAACAACCCGATATCGATGGTCTTTTAATCGGAGGCGCATCA
CTTTCTCTTGAAACTTTTGTTGAAATTGTGAATGATGGTGGGTCAATATTCAATCTGAAGGCAAAATTACTATGA

Upstream 100 bases:

>100_bases
GCTTGAATAAAAATGAAATTTCTTGAGTTTCATCTCTTCCTAGATTAGGATAACATATAAATATTTTCTTAAGAATTTGA
GATTAAAAGGAGAGCGTCGG

Downstream 100 bases:

>100_bases
CGTTCCTATATTTTTTTACTATTACGCTCTTTTTGATTATTTGCGCATTACTTTGTGCAGTTATTTTGATGCAAGAAAGC
AAAAGTTCTGGGCTCGGAGC

Product: triose-phosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSPSHRPVVITGNWKMYKTVKEACTFAKALLPVVEVSPIQVWIAVPFTAIYPVKQEIRNSRLVIGAQNMNDASEGAFTGE
IAGKMVKEAGASFVLLGHSERRHLYKEDNAFINRKVKKALEIGLTPVLCVGETFEERKSGETQQIIRTQIQECLAGLTAE
DLKTLIIAYEPVWAIGNGQNAKPEGAQEIHQFCRKIIKEIFSEELAEQIVIQYGGSVNPSNAISLLKQPDIDGLLIGGAS
LSLETFVEIVNDGGSIFNLKAKLL

Sequences:

>Translated_264_residues
MSPSHRPVVITGNWKMYKTVKEACTFAKALLPVVEVSPIQVWIAVPFTAIYPVKQEIRNSRLVIGAQNMNDASEGAFTGE
IAGKMVKEAGASFVLLGHSERRHLYKEDNAFINRKVKKALEIGLTPVLCVGETFEERKSGETQQIIRTQIQECLAGLTAE
DLKTLIIAYEPVWAIGNGQNAKPEGAQEIHQFCRKIIKEIFSEELAEQIVIQYGGSVNPSNAISLLKQPDIDGLLIGGAS
LSLETFVEIVNDGGSIFNLKAKLL
>Mature_263_residues
SPSHRPVVITGNWKMYKTVKEACTFAKALLPVVEVSPIQVWIAVPFTAIYPVKQEIRNSRLVIGAQNMNDASEGAFTGEI
AGKMVKEAGASFVLLGHSERRHLYKEDNAFINRKVKKALEIGLTPVLCVGETFEERKSGETQQIIRTQIQECLAGLTAED
LKTLIIAYEPVWAIGNGQNAKPEGAQEIHQFCRKIIKEIFSEELAEQIVIQYGGSVNPSNAISLLKQPDIDGLLIGGASL
SLETFVEIVNDGGSIFNLKAKLL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=251, Percent_Identity=39.0438247011952, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI226529917, Length=251, Percent_Identity=39.0438247011952, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1790353, Length=246, Percent_Identity=43.0894308943089, Blast_Score=197, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17536593, Length=250, Percent_Identity=43.2, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=38.6178861788618, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=43.3198380566802, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=43.3198380566802, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=43.3198380566802, Blast_Score=198, Evalue=4e-51,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PARUW (Q6MD24)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007800.1
- HSSP:   P36204
- ProteinModelPortal:   Q6MD24
- SMR:   Q6MD24
- STRING:   Q6MD24
- GeneID:   2780469
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0801
- NMPDR:   fig|264201.1.peg.801
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q6MD24
- ProtClustDB:   CLSK2762259
- BioCyc:   CPRO264201:PC0801-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28964; Mature: 28833

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 98-98 ACT_SITE 170-170 BINDING 13-13 BINDING 15-15

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSHRPVVITGNWKMYKTVKEACTFAKALLPVVEVSPIQVWIAVPFTAIYPVKQEIRNS
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCC
RLVIGAQNMNDASEGAFTGEIAGKMVKEAGASFVLLGHSERRHLYKEDNAFINRKVKKAL
EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCHHHHHHHHHHHH
EIGLTPVLCVGETFEERKSGETQQIIRTQIQECLAGLTAEDLKTLIIAYEPVWAIGNGQN
HHCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHEEHEEECCEEEECCCCC
AKPEGAQEIHQFCRKIIKEIFSEELAEQIVIQYGGSVNPSNAISLLKQPDIDGLLIGGAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCC
LSLETFVEIVNDGGSIFNLKAKLL
CCHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
SPSHRPVVITGNWKMYKTVKEACTFAKALLPVVEVSPIQVWIAVPFTAIYPVKQEIRNS
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCC
RLVIGAQNMNDASEGAFTGEIAGKMVKEAGASFVLLGHSERRHLYKEDNAFINRKVKKAL
EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCHHHHHHHHHHHH
EIGLTPVLCVGETFEERKSGETQQIIRTQIQECLAGLTAEDLKTLIIAYEPVWAIGNGQN
HHCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHEEHEEECCEEEECCCCC
AKPEGAQEIHQFCRKIIKEIFSEELAEQIVIQYGGSVNPSNAISLLKQPDIDGLLIGGAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCC
LSLETFVEIVNDGGSIFNLKAKLL
CCHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA