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The map label for this gene is ptsI [H]

Identifier: 46446110

GI number: 46446110

Start: 611040

End: 612818

Strand: Direct

Name: ptsI [H]

Synonym: pc0476

Alternate gene names: 46446110

Gene position: 611040-612818 (Clockwise)

Preceding gene: 46446109

Following gene: 46446113

Centisome position: 25.31

GC content: 37.1

Gene sequence:

>1779_bases
ATGCAGCTCAAGGCTGAAGAGATTATTTTAAAAGGCTCTCCCATCTGCCGTGGCATTGCAATTGGTCAAACATATTTTCT
CGATCGTGATGAATTTACTGTTTGTGAAAAGCAGATTGATTCTTCCGATTCTGAAAAAGAGGTTGAACGTTATCGTCACG
CCCTTTTTAGAAGTAGGCAAGATATCAAGCGATTACAAACACAACTTGAATCGGAATCAGCACAAGAAGCTATCCTTATC
TTAGAGGCTCAGCTAGAGATTCTACAAGATCCTTTATTGACAACTGAAATGGAATTGAATATTCGACAGGCTAATCAAAA
TGCTGAGTTTGTTTTTCAGCAATCTTTGGTTAAGCTTCAAACTCGTTTTGAATCTTTAAAAGATCCCTTTTTTGCAGAAA
GGTTTAAAGATTTACAAGATGTCTCACAAAGAGTCTTTAGCTATCTTTACGAAAGTGGAAGTTTGTCTTTAAAAGATGTT
CCTTACAATTCAATCGTTTGTGGAGTGGAATTAACAGCTTCGGATACTGCGGAAGCTAAAGTTTACCATGTGGGAGCATT
TTTAACGGAAAGTGGAGGAGCAACTTCTCATGCTGCGATTGTTGCTAAAGCAAAAGGAATTCCTTTTATTTCCAATATTA
ATCTTCATCTAATTAGAGAAAATGACAATCAAACGATTATTGTTGACGGGCGAACGGGTCAAGTGATCCTTAACCCAACG
GATGAGACTCTTCATCGTTACGAAACGTTGAAGAGAAAAATGCATACGCAAATTACTGCATTTGAAAACGATGTACATTG
GCCGGCTGAGACTTGTGATGGATATACCGTTAGGTTATATGGAAATTTAGATGATAGTCATCAAGTTGATTTGCTTAAGC
AATTAGGTGGCCAAGGGATAGGCCTTTTTCGGTCTGAATATATTTTTCTTCCTCAAAAAGAAATCCCAGGCGAAGAAGAA
CAATTCAACATTTATTCGCAAATTGTGAAAAAAATGGATGGCTTGCCTGTCGTCATTCGAACATTTGATTTAGGGGGAGA
TAAGGCAACTTTTCACGTTCCTTCAACAACTCAAAAAAATCCTTTTCTTGGTTCAAGGGCAACACGGTATTTTTTAAGGG
AACAGAAGTTATTGAAAAGTCAATTAAGAGCTATTGTCAGAGCAAGTATTTATGGGCAGGTTCGTATTTTGTTTCCCATG
ATTGCGACAGTTGCCGAATTAAGAGAAGCAAAAAAAATTCTGCAAGAAGTTTGTCAAGAACTGCACTTTGACCAACATAT
TCCGATGGGTTGCATGATCGAGGTTCCCTCGGCAGCTATGGTGGTGGATCATTTTGTTAAAGAATGTGATTTTCTTTCGA
TTGGGACGAATGATTTAGTTCAATATGCCTTAGCAATTGATCGGAAAGATCACTTGTTAAATGATTTTTATGAACCAACT
GACCCGAGCATTATACGCTTGATCAAGTTAGTTATTTCTGAAGGAAATAAAGCTCATGTCCCTGTTTCCGTATGTGGAGA
AATCGCCTCTGATCCTCGTTTTATTCCTTTATTACTAGGACTTGGAGTTCAAGAATTATCTGTAGCTCCACGCTATTTAC
CTGTTATCAAAAATGTTATTCGTAGTACGTATATTGTTGATGCTGTTCAATTGGCGGACTTAGCTTTACATCTGACCACT
GCACAAGAAGTTTTAAACTTGTTAATCCAGGAATATCAAAAAAGTGTGCCTCATGATTTATTTTATAATGTATCTTATCC
TCCTCAAACATATATTTAA

Upstream 100 bases:

>100_bases
AAAAAATTCTAAAATTACAATAGCAGTGGAAGGGGAAGATGCAAATGACACCATGGATAAACTGATTGAGGCGTTTGAGT
CTCAATTCGGAGAGTAAGTA

Downstream 100 bases:

>100_bases
ACTTCGGATACAAAGTAATTTTGTTTTGAAATTAAGAAAACATTACCTGTAAAATGTTTTCTCCTCTAGCATGGAATTAG
GCTAGAAGTTAAAGCATATT

Product: putative phosphoenolpyruvate-protein phosphotransferase (PTS enzyme I)

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MQLKAEEIILKGSPICRGIAIGQTYFLDRDEFTVCEKQIDSSDSEKEVERYRHALFRSRQDIKRLQTQLESESAQEAILI
LEAQLEILQDPLLTTEMELNIRQANQNAEFVFQQSLVKLQTRFESLKDPFFAERFKDLQDVSQRVFSYLYESGSLSLKDV
PYNSIVCGVELTASDTAEAKVYHVGAFLTESGGATSHAAIVAKAKGIPFISNINLHLIRENDNQTIIVDGRTGQVILNPT
DETLHRYETLKRKMHTQITAFENDVHWPAETCDGYTVRLYGNLDDSHQVDLLKQLGGQGIGLFRSEYIFLPQKEIPGEEE
QFNIYSQIVKKMDGLPVVIRTFDLGGDKATFHVPSTTQKNPFLGSRATRYFLREQKLLKSQLRAIVRASIYGQVRILFPM
IATVAELREAKKILQEVCQELHFDQHIPMGCMIEVPSAAMVVDHFVKECDFLSIGTNDLVQYALAIDRKDHLLNDFYEPT
DPSIIRLIKLVISEGNKAHVPVSVCGEIASDPRFIPLLLGLGVQELSVAPRYLPVIKNVIRSTYIVDAVQLADLALHLTT
AQEVLNLLIQEYQKSVPHDLFYNVSYPPQTYI

Sequences:

>Translated_592_residues
MQLKAEEIILKGSPICRGIAIGQTYFLDRDEFTVCEKQIDSSDSEKEVERYRHALFRSRQDIKRLQTQLESESAQEAILI
LEAQLEILQDPLLTTEMELNIRQANQNAEFVFQQSLVKLQTRFESLKDPFFAERFKDLQDVSQRVFSYLYESGSLSLKDV
PYNSIVCGVELTASDTAEAKVYHVGAFLTESGGATSHAAIVAKAKGIPFISNINLHLIRENDNQTIIVDGRTGQVILNPT
DETLHRYETLKRKMHTQITAFENDVHWPAETCDGYTVRLYGNLDDSHQVDLLKQLGGQGIGLFRSEYIFLPQKEIPGEEE
QFNIYSQIVKKMDGLPVVIRTFDLGGDKATFHVPSTTQKNPFLGSRATRYFLREQKLLKSQLRAIVRASIYGQVRILFPM
IATVAELREAKKILQEVCQELHFDQHIPMGCMIEVPSAAMVVDHFVKECDFLSIGTNDLVQYALAIDRKDHLLNDFYEPT
DPSIIRLIKLVISEGNKAHVPVSVCGEIASDPRFIPLLLGLGVQELSVAPRYLPVIKNVIRSTYIVDAVQLADLALHLTT
AQEVLNLLIQEYQKSVPHDLFYNVSYPPQTYI
>Mature_592_residues
MQLKAEEIILKGSPICRGIAIGQTYFLDRDEFTVCEKQIDSSDSEKEVERYRHALFRSRQDIKRLQTQLESESAQEAILI
LEAQLEILQDPLLTTEMELNIRQANQNAEFVFQQSLVKLQTRFESLKDPFFAERFKDLQDVSQRVFSYLYESGSLSLKDV
PYNSIVCGVELTASDTAEAKVYHVGAFLTESGGATSHAAIVAKAKGIPFISNINLHLIRENDNQTIIVDGRTGQVILNPT
DETLHRYETLKRKMHTQITAFENDVHWPAETCDGYTVRLYGNLDDSHQVDLLKQLGGQGIGLFRSEYIFLPQKEIPGEEE
QFNIYSQIVKKMDGLPVVIRTFDLGGDKATFHVPSTTQKNPFLGSRATRYFLREQKLLKSQLRAIVRASIYGQVRILFPM
IATVAELREAKKILQEVCQELHFDQHIPMGCMIEVPSAAMVVDHFVKECDFLSIGTNDLVQYALAIDRKDHLLNDFYEPT
DPSIIRLIKLVISEGNKAHVPVSVCGEIASDPRFIPLLLGLGVQELSVAPRYLPVIKNVIRSTYIVDAVQLADLALHLTT
AQEVLNLLIQEYQKSVPHDLFYNVSYPPQTYI

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=556, Percent_Identity=32.9136690647482, Blast_Score=332, Evalue=5e-92,
Organism=Escherichia coli, GI1789193, Length=570, Percent_Identity=29.1228070175439, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI1788726, Length=582, Percent_Identity=28.1786941580756, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI48994992, Length=511, Percent_Identity=30.5283757338552, Blast_Score=235, Evalue=7e-63,
Organism=Escherichia coli, GI1787994, Length=391, Percent_Identity=23.2736572890026, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 67067; Mature: 67067

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00742 PEP_ENZYMES_2 ; PS00449 ATPASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKAEEIILKGSPICRGIAIGQTYFLDRDEFTVCEKQIDSSDSEKEVERYRHALFRSRQ
CCCCHHHEEECCCCCCCEEEECCEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCHH
DIKRLQTQLESESAQEAILILEAQLEILQDPLLTTEMELNIRQANQNAEFVFQQSLVKLQ
HHHHHHHHHHHCCHHHEEEHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
TRFESLKDPFFAERFKDLQDVSQRVFSYLYESGSLSLKDVPYNSIVCGVELTASDTAEAK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCCCCCCE
VYHVGAFLTESGGATSHAAIVAKAKGIPFISNINLHLIRENDNQTIIVDGRTGQVILNPT
EEEEEEEEECCCCCCCHHEEEEECCCCCEEECCEEEEEEECCCCEEEEECCCCEEEECCC
DETLHRYETLKRKMHTQITAFENDVHWPAETCDGYTVRLYGNLDDSHQVDLLKQLGGQGI
HHHHHHHHHHHHHHHHHHEEECCCCCCCHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCE
GLFRSEYIFLPQKEIPGEEEQFNIYSQIVKKMDGLPVVIRTFDLGGDKATFHVPSTTQKN
EEECCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCCCCC
PFLGSRATRYFLREQKLLKSQLRAIVRASIYGQVRILFPMIATVAELREAKKILQEVCQE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHFDQHIPMGCMIEVPSAAMVVDHFVKECDFLSIGTNDLVQYALAIDRKDHLLNDFYEPT
HCCCCCCCCCEEEECCCHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCHHHHHHHCCCCC
DPSIIRLIKLVISEGNKAHVPVSVCGEIASDPRFIPLLLGLGVQELSVAPRYLPVIKNVI
CHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHCCCHHHHHCCCHHHHHHHHHH
RSTYIVDAVQLADLALHLTTAQEVLNLLIQEYQKSVPHDLFYNVSYPPQTYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MQLKAEEIILKGSPICRGIAIGQTYFLDRDEFTVCEKQIDSSDSEKEVERYRHALFRSRQ
CCCCHHHEEECCCCCCCEEEECCEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCHH
DIKRLQTQLESESAQEAILILEAQLEILQDPLLTTEMELNIRQANQNAEFVFQQSLVKLQ
HHHHHHHHHHHCCHHHEEEHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
TRFESLKDPFFAERFKDLQDVSQRVFSYLYESGSLSLKDVPYNSIVCGVELTASDTAEAK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCCCCCCE
VYHVGAFLTESGGATSHAAIVAKAKGIPFISNINLHLIRENDNQTIIVDGRTGQVILNPT
EEEEEEEEECCCCCCCHHEEEEECCCCCEEECCEEEEEEECCCCEEEEECCCCEEEECCC
DETLHRYETLKRKMHTQITAFENDVHWPAETCDGYTVRLYGNLDDSHQVDLLKQLGGQGI
HHHHHHHHHHHHHHHHHHEEECCCCCCCHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCE
GLFRSEYIFLPQKEIPGEEEQFNIYSQIVKKMDGLPVVIRTFDLGGDKATFHVPSTTQKN
EEECCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCCCCC
PFLGSRATRYFLREQKLLKSQLRAIVRASIYGQVRILFPMIATVAELREAKKILQEVCQE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHFDQHIPMGCMIEVPSAAMVVDHFVKECDFLSIGTNDLVQYALAIDRKDHLLNDFYEPT
HCCCCCCCCCEEEECCCHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCHHHHHHHCCCCC
DPSIIRLIKLVISEGNKAHVPVSVCGEIASDPRFIPLLLGLGVQELSVAPRYLPVIKNVI
CHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHCCCHHHHHCCCHHHHHHHHHH
RSTYIVDAVQLADLALHLTTAQEVLNLLIQEYQKSVPHDLFYNVSYPPQTYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7686067; 9384377; 10568751; 2497294; 8449881; 1495386 [H]