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The map label for this gene is rpoA

Identifier: 46446067

GI number: 46446067

Start: 560293

End: 561408

Strand: Direct

Name: rpoA

Synonym: pc0433

Alternate gene names: 46446067

Gene position: 560293-561408 (Clockwise)

Preceding gene: 46446066

Following gene: 46446068

Centisome position: 23.21

GC content: 38.08

Gene sequence:

>1116_bases
ATGTCAGTAAAATACGGCAAATTTGAAATGCCTCATAAGATTACAGTAGATCAAGAGTCTCCTGAATCTAATTTTGCCCG
TTATGTGGCTGAGCCTTTCGAAAGAGGATTTGGACATACAATTGGAAATGCATTGCGTAGAATGATGTTGTCGTCTTTAG
AAGCTCCAGCTATCATTTCTGTTCGCGTAGAGGGCATTCCTCACGAATATATGGCTATTGAAGGAATTTCTGAGGATATG
ACTAATATTATCCTCAACTTTAAAGGAGCTTTGCTACGCAAACTTCCTACTGAAGAAACTCCTAGAGATACGCGTATTTT
GACAAAAGTCGTCGAAGTGACACAAGACGACTTAGATCGCAATCAGGGGCAATACTGCGTTACATTGCAAGATGTGGTTC
AAGAAGGCAATTTTGAAATTGTTAATCCAGAATTACATCTTTTTACTGTGACTAAGCCTATGCGTCGTCAAGTAGATCTT
CGAATTGCCTTTGGACGCGGTTATGTCCCGTCTGAGCGTCACGTGGTACGTGATAAAACGTCCGATGAAATTTTAGTTGA
TGCAGCTTTTTCACCTGTTCGTTTAATCAATTATTTTATCGAGAATACACGGGTAGGTCAAGATACTGATTTTGATCGAC
TCATCATGGAAGTTACCACTGATGGGCGTATTACTCCTGCTGAAGCTTTGAGTTTTGCAGTTCAAATTGGGCTTAAACAT
TTTGAAGTGTTTAATCAATTTAATAATTACGCCCTTTCTTTTGACGAAAAAGATGGAGATCGTAACGGTGATCAAGATGA
GTTAATGGATAAACTTTCTTTAGGAATTGATGAAATCGAGTTATCAGTTCGTTCAGCTAACTGCTTAACTGGTGCTAATA
TCGAGACACTCGCAGAATTGGTTTGCATTCCAGAACGTAGGATGTTAGAATTCAGAAACTTCGGTAAAAAATCCTTAAAT
GAAATTAAAGCTAAACTTCATGAGATGTCATTGCACTTAGGCATGGACTTGAGTCGTTTTGGGGTTTCACCTGATAATGT
CAAAGATAAAATCAAGCAGTACCGTGAAGAAAAGAAAAAGAAAAAAGAATTAGTTAAACACGAAGATGCTAAGTAG

Upstream 100 bases:

>100_bases
GTATCAGCATTAAAAGGCTTATTAATAGACTGCTCATTTGCTTCATACGATCAAGAGCCAAACGTTCATCTCAACTATTA
CTATTTTTAGGAGTCACTTC

Downstream 100 bases:

>100_bases
GTAAACAGTTATGAGACACCTCAATCAAACATGTAAGCTCAATCGAACCACGTCTCATAGACGTTGCATGTTCGCCAACA
TGTTAAAATCTTTAATATCG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSVKYGKFEMPHKITVDQESPESNFARYVAEPFERGFGHTIGNALRRMMLSSLEAPAIISVRVEGIPHEYMAIEGISEDM
TNIILNFKGALLRKLPTEETPRDTRILTKVVEVTQDDLDRNQGQYCVTLQDVVQEGNFEIVNPELHLFTVTKPMRRQVDL
RIAFGRGYVPSERHVVRDKTSDEILVDAAFSPVRLINYFIENTRVGQDTDFDRLIMEVTTDGRITPAEALSFAVQIGLKH
FEVFNQFNNYALSFDEKDGDRNGDQDELMDKLSLGIDEIELSVRSANCLTGANIETLAELVCIPERRMLEFRNFGKKSLN
EIKAKLHEMSLHLGMDLSRFGVSPDNVKDKIKQYREEKKKKKELVKHEDAK

Sequences:

>Translated_371_residues
MSVKYGKFEMPHKITVDQESPESNFARYVAEPFERGFGHTIGNALRRMMLSSLEAPAIISVRVEGIPHEYMAIEGISEDM
TNIILNFKGALLRKLPTEETPRDTRILTKVVEVTQDDLDRNQGQYCVTLQDVVQEGNFEIVNPELHLFTVTKPMRRQVDL
RIAFGRGYVPSERHVVRDKTSDEILVDAAFSPVRLINYFIENTRVGQDTDFDRLIMEVTTDGRITPAEALSFAVQIGLKH
FEVFNQFNNYALSFDEKDGDRNGDQDELMDKLSLGIDEIELSVRSANCLTGANIETLAELVCIPERRMLEFRNFGKKSLN
EIKAKLHEMSLHLGMDLSRFGVSPDNVKDKIKQYREEKKKKKELVKHEDAK
>Mature_370_residues
SVKYGKFEMPHKITVDQESPESNFARYVAEPFERGFGHTIGNALRRMMLSSLEAPAIISVRVEGIPHEYMAIEGISEDMT
NIILNFKGALLRKLPTEETPRDTRILTKVVEVTQDDLDRNQGQYCVTLQDVVQEGNFEIVNPELHLFTVTKPMRRQVDLR
IAFGRGYVPSERHVVRDKTSDEILVDAAFSPVRLINYFIENTRVGQDTDFDRLIMEVTTDGRITPAEALSFAVQIGLKHF
EVFNQFNNYALSFDEKDGDRNGDQDELMDKLSLGIDEIELSVRSANCLTGANIETLAELVCIPERRMLEFRNFGKKSLNE
IKAKLHEMSLHLGMDLSRFGVSPDNVKDKIKQYREEKKKKKELVKHEDAK

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=340, Percent_Identity=37.0588235294118, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_PARUW (Q6ME42)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007432.1
- ProteinModelPortal:   Q6ME42
- STRING:   Q6ME42
- GeneID:   2781452
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0433
- NMPDR:   fig|264201.1.peg.433
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   CPRO264201:PC0433-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 42445; Mature: 42314

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKYGKFEMPHKITVDQESPESNFARYVAEPFERGFGHTIGNALRRMMLSSLEAPAIIS
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
VRVEGIPHEYMAIEGISEDMTNIILNFKGALLRKLPTEETPRDTRILTKVVEVTQDDLDR
EEECCCCCHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
NQGQYCVTLQDVVQEGNFEIVNPELHLFTVTKPMRRQVDLRIAFGRGYVPSERHVVRDKT
CCCCEEEEHHHHHHCCCEEEECCCEEEEEECHHHHHCEEEEEEECCCCCCCCCCEECCCC
SDEILVDAAFSPVRLINYFIENTRVGQDTDFDRLIMEVTTDGRITPAEALSFAVQIGLKH
CCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHH
FEVFNQFNNYALSFDEKDGDRNGDQDELMDKLSLGIDEIELSVRSANCLTGANIETLAEL
HHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHEEEEECCCCCCCCCHHHHHHH
VCIPERRMLEFRNFGKKSLNEIKAKLHEMSLHLGMDLSRFGVSPDNVKDKIKQYREEKKK
HHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHH
KKELVKHEDAK
HHHHHHCCCCC
>Mature Secondary Structure 
SVKYGKFEMPHKITVDQESPESNFARYVAEPFERGFGHTIGNALRRMMLSSLEAPAIIS
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
VRVEGIPHEYMAIEGISEDMTNIILNFKGALLRKLPTEETPRDTRILTKVVEVTQDDLDR
EEECCCCCHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
NQGQYCVTLQDVVQEGNFEIVNPELHLFTVTKPMRRQVDLRIAFGRGYVPSERHVVRDKT
CCCCEEEEHHHHHHCCCEEEECCCEEEEEECHHHHHCEEEEEEECCCCCCCCCCEECCCC
SDEILVDAAFSPVRLINYFIENTRVGQDTDFDRLIMEVTTDGRITPAEALSFAVQIGLKH
CCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHH
FEVFNQFNNYALSFDEKDGDRNGDQDELMDKLSLGIDEIELSVRSANCLTGANIETLAEL
HHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHEEEEECCCCCCCCCHHHHHHH
VCIPERRMLEFRNFGKKSLNEIKAKLHEMSLHLGMDLSRFGVSPDNVKDKIKQYREEKKK
HHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHH
KKELVKHEDAK
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA