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The map label for this gene is purT

Identifier: 45659230

GI number: 45659230

Start: 4186510

End: 4187673

Strand: Reverse

Name: purT

Synonym: LIC13413

Alternate gene names: 45659230

Gene position: 4187673-4186510 (Counterclockwise)

Preceding gene: 45659231

Following gene: 45659228

Centisome position: 97.91

GC content: 39.6

Gene sequence:

>1164_bases
ATGAAAAAGAAAATCCTTCTCTTAGGTTCCGGTGAACTCGGTAAAGAATTTGTAATTGCGGCTCAGAGGTTGGGGCAATA
TGTTATTGCAGTTGATTCTTACGATGATGCCCCTGCTATGCAAGTCGCTCACGAAAAAGAAATCATCAATATGTTAGACG
GAAACCTTTTGGATCAGATTATAGCGAAACATAAACCGGATTTGATTGTTCCAGAAATCGAAGCGATTAAAACAGAACGT
TTTTACGAATATGAAAAACAGGGTTATCAAGTTGTTCCCTCGGCAAAAGCTGCCAATTTTACTATGAATCGTAAATCGAT
CCGAGATCTTGCTGCTAAGGATCTAAAACTTCTCACAGCTAAATATGCATATGCTTCTTCTATAGACGAACTGATAAAAG
CAACGGAAATATTAGGTTTTCCTTGTGTTGTAAAGCCACTCATGTCTTCTTCTGGAAAAGGGCAATCGGTTATCCAGTCT
CGAGAAGAAATTTCTAAAGCTTGGGAAGCGTCTCAAACAAAAGGTCGTGCCGGTGCCGCAGAAATTATCGTGGAAGAGTT
TATTCCTTTTGAATCGGAAATTACTCTTTTAACGGTTACTCAGAAAAATGGTAAAACTTTATTTTGCCCACCGATCGGCC
ATCGACAAGAAAGAGGGGATTATCAAGAAAGTTGGCAACCGGCCGCAATCTCGGAAGTTCAACTTAAAGAAGCCCAGAGG
ATGGCAGACGCAGTGACAAAAGAACTGACTGGTTTTGGCATTTGGGGAGTGGAATTTTTTTTAACCGAGGATAAGGTTTA
TTTTTCGGAACTTTCTCCAAGGCCGCACGATACTGGAATGGTTACGTTAGCCGGAACACAAAATTTCAACGAGTTCGAAC
TTCACCTTAGAGCGATTTTAGGAATTCCAATTTTAGAAATTACTCTGGAAAGAAAAGGTGCAAGCGCTGTAATTCTTGCG
AGTACTGAAAATAAAACCCCCGAAATTAGTGGGTTAGATATTGCATCTGGAATGTCTGAATCAGATTTTAGAATTTTTGG
AAAACCTATTACAAGGCCGTATCGCAGAATGGGTGTTACTCTTTCTTATTCTACAAAAGGAGAGGAAATTTCTTCTCTTC
GTAAACGTGCGGTTCTTTTAGCTTCTAAAATCAAAGTCGATTGA

Upstream 100 bases:

>100_bases
GTAAGAACAGCTATAATTTCAAGTTGGACAATAGAATTTTGTTGTTGGTTTTAATTTTCTCTTTGATCTTTCTGTTCATT
CTTTCAATCTGTCCCAGACT

Downstream 100 bases:

>100_bases
ATGAATCTCTATAAAATTCAAGGTAACGATTTTCAATTTAGTTTTGCGTAAAATTTGTGTTTGGTGATTCGTGCTACAAA
ATTGTAATTTCGTGTCAAAG

Product: phosphoribosylglycinamide formyltransferase 2

Products: tetrahydrofolate; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKKKILLLGSGELGKEFVIAAQRLGQYVIAVDSYDDAPAMQVAHEKEIINMLDGNLLDQIIAKHKPDLIVPEIEAIKTER
FYEYEKQGYQVVPSAKAANFTMNRKSIRDLAAKDLKLLTAKYAYASSIDELIKATEILGFPCVVKPLMSSSGKGQSVIQS
REEISKAWEASQTKGRAGAAEIIVEEFIPFESEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAAISEVQLKEAQR
MADAVTKELTGFGIWGVEFFLTEDKVYFSELSPRPHDTGMVTLAGTQNFNEFELHLRAILGIPILEITLERKGASAVILA
STENKTPEISGLDIASGMSESDFRIFGKPITRPYRRMGVTLSYSTKGEEISSLRKRAVLLASKIKVD

Sequences:

>Translated_387_residues
MKKKILLLGSGELGKEFVIAAQRLGQYVIAVDSYDDAPAMQVAHEKEIINMLDGNLLDQIIAKHKPDLIVPEIEAIKTER
FYEYEKQGYQVVPSAKAANFTMNRKSIRDLAAKDLKLLTAKYAYASSIDELIKATEILGFPCVVKPLMSSSGKGQSVIQS
REEISKAWEASQTKGRAGAAEIIVEEFIPFESEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAAISEVQLKEAQR
MADAVTKELTGFGIWGVEFFLTEDKVYFSELSPRPHDTGMVTLAGTQNFNEFELHLRAILGIPILEITLERKGASAVILA
STENKTPEISGLDIASGMSESDFRIFGKPITRPYRRMGVTLSYSTKGEEISSLRKRAVLLASKIKVD
>Mature_387_residues
MKKKILLLGSGELGKEFVIAAQRLGQYVIAVDSYDDAPAMQVAHEKEIINMLDGNLLDQIIAKHKPDLIVPEIEAIKTER
FYEYEKQGYQVVPSAKAANFTMNRKSIRDLAAKDLKLLTAKYAYASSIDELIKATEILGFPCVVKPLMSSSGKGQSVIQS
REEISKAWEASQTKGRAGAAEIIVEEFIPFESEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAAISEVQLKEAQR
MADAVTKELTGFGIWGVEFFLTEDKVYFSELSPRPHDTGMVTLAGTQNFNEFELHLRAILGIPILEITLERKGASAVILA
STENKTPEISGLDIASGMSESDFRIFGKPITRPYRRMGVTLSYSTKGEEISSLRKRAVLLASKIKVD

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=383, Percent_Identity=49.0861618798956, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1786733, Length=326, Percent_Identity=24.8466257668712, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=25.8855585831063, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_LEPIC (Q72LY0)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_003316.1
- HSSP:   P33221
- ProteinModelPortal:   Q72LY0
- GeneID:   2771152
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC13413
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- BioCyc:   LINT267671:LIC_13413-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.2

Molecular weight: Translated: 42953; Mature: 42953

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 72-72 BINDING 104-104 BINDING 145-145 BINDING 193-193 BINDING 258-258 BINDING 270-270 BINDING 277-277 BINDING 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKILLLGSGELGKEFVIAAQRLGQYVIAVDSYDDAPAMQVAHEKEIINMLDGNLLDQI
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
IAKHKPDLIVPEIEAIKTERFYEYEKQGYQVVPSAKAANFTMNRKSIRDLAAKDLKLLTA
HHHCCCCEECCCHHHHHHHHHHHHHHCCCEECCCCCCCCEEECHHHHHHHHHHHHHHHHH
KYAYASSIDELIKATEILGFPCVVKPLMSSSGKGQSVIQSREEISKAWEASQTKGRAGAA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
EIIVEEFIPFESEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAAISEVQLKEAQR
HHHHHHHCCCCCCEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCHHHHHHHHHHHH
MADAVTKELTGFGIWGVEFFLTEDKVYFSELSPRPHDTGMVTLAGTQNFNEFELHLRAIL
HHHHHHHHHCCCEEEEEEEEEECCEEEEHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
GIPILEITLERKGASAVILASTENKTPEISGLDIASGMSESDFRIFGKPITRPYRRMGVT
CCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHCCCCCCCCEEECCCCCCHHHHHCCE
LSYSTKGEEISSLRKRAVLLASKIKVD
EEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKKILLLGSGELGKEFVIAAQRLGQYVIAVDSYDDAPAMQVAHEKEIINMLDGNLLDQI
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
IAKHKPDLIVPEIEAIKTERFYEYEKQGYQVVPSAKAANFTMNRKSIRDLAAKDLKLLTA
HHHCCCCEECCCHHHHHHHHHHHHHHCCCEECCCCCCCCEEECHHHHHHHHHHHHHHHHH
KYAYASSIDELIKATEILGFPCVVKPLMSSSGKGQSVIQSREEISKAWEASQTKGRAGAA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
EIIVEEFIPFESEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAAISEVQLKEAQR
HHHHHHHCCCCCCEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCHHHHHHHHHHHH
MADAVTKELTGFGIWGVEFFLTEDKVYFSELSPRPHDTGMVTLAGTQNFNEFELHLRAIL
HHHHHHHHHCCCEEEEEEEEEECCEEEEHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
GIPILEITLERKGASAVILASTENKTPEISGLDIASGMSESDFRIFGKPITRPYRRMGVT
CCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHCCCCCCCCEEECCCCCCHHHHHCCE
LSYSTKGEEISSLRKRAVLLASKIKVD
EEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 10-formyltetrahydrofolate; N1-(5-phospho-D-ribosyl)glycinamide

Specific reaction: 10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA