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The map label for this gene is gltX

Identifier: 45659164

GI number: 45659164

Start: 4100683

End: 4102248

Strand: Reverse

Name: gltX

Synonym: LIC13345

Alternate gene names: 45659164

Gene position: 4102248-4100683 (Counterclockwise)

Preceding gene: 45659165

Following gene: 45659163

Centisome position: 95.91

GC content: 37.8

Gene sequence:

>1566_bases
ATGAATCAAAGTAAAGAAGTTCGTACCCGATTTGCGCCGTCTCCTAGCGGATTTTTACACGTAGGTGGAGCGCGAACCGC
TCTTTTTAATTATTTATACGCAAAGTCTCAAGGCGGAAAATTCATTTTAAGAATCGAAGATACGGATCAAAACAGATCCA
CGGAAGATTCTTTTAAAATCATATTAGAATCTTTGAAATGGTTGGGAGTAAATTGGGACGAAGGTCCTGAGGTTGGAGGA
GAATATGGTCCCTATATTCAAAGCCAACGTTTGAATATTTATAAAGAATATACTGAAAAACTTCTGAAGGAAAAAAAGGC
TTATCGTTGTTTTTGCACTCAAGAAGAATTAGAAGCCAAAAAAAAACAATCGGAAGCGATGGGAGTTCCTTATGTCTATG
ATGGACTTCATGCGAATATGTCTGATGAGGAAGTGCAAGAAAAACTCAAACAAGGGATTCCATATTCGGTTCGATTTAAA
ACTCCTTCTAAAACTTTGATCATCAATGACATTATTCAAGGAAAAGTAAAATTCGAAACAAAGTTGATCGGCGATTTTAT
TATCGTTAAGTCGGATGGATTTCCTTCTTACAATTACGCGGTCGTAGTAGACGACGCCCTTATGAAAATCACACATGTGA
TCCGAGGGGTGGGCCATCTTTCCAATACTCCGAGACAAATTTTATTATACGAAGCACTGGGTTATAATATTCCAGAATTT
GCACATGCTTCTGAAATCGTTGGAATGGATGGAAAAAAACTTTCTAAAAGAGCGGGAGCTACTTCGATTTTAGCGTTTAG
GGATTTAGGTTATTTACCAGAAACGTTTCGAAATTATATGGCTCTTCTGGGATGGACTTCTACAGACGGAAGAGAGTTTC
TTCCCGGAGATGAACTCGAAAAAATTTTTGACGTTCATCGTTGTTCTAAATCTCCTTCTACGTTTGACGTATTTAAAAAG
CCGAAAGGTAGTGATGAAGAAGTTGTTACGAACTTTTCGGATTTGACTCAGATTGCGGAGGCGATGAATCCTAAATCCAA
ACTCAATTGGTTGTCCAATCGAACGATTCGGGATTTGAATATATCCAAAGTATTAGAGAATCTTCTTCCCTTCCTGATGG
ATAGAAAAGATATTCCTGCCGAAGTTAAAAATGTAAACAACCCAATCCTTACTTCTATCGTAGAATCTGTAAGAGTGTAT
TTGGATAATCTAACACAGGCTCCGGATTATGTTGCAGAATTTTTTGTTACTGACTTAAAGATCCAGTCTGAAGAAACCAT
CGGGTTTTTAAAGGACGGCGAAGGTCCCAAAGTTGTAAATGAATTTTATGAAATATTGAAGAATTCCGATCCAAAAACCG
ATGAAGATTATAAGAATCTAATGTCTAAAGTCGGAGAGGTGACCGGACAAAAAGGAAAAACTCTTTATATGCCGATTCGG
GCAGCTACTACCGGAAAGTCGGCCGGATTGGAGTTACCGATTTTATTTCCACTTTTGGGGAAAGAAAAGCTACTCCAAAG
AATTGAAAAAACTTCAAAAGAAACCGGAATTTCGTTGTCGAATTGA

Upstream 100 bases:

>100_bases
TTTCATTTTACGATCTAATTACAAATAAACCATTTACCATAACAGCCAATTTCAAAAATTATCCTATCATAAGAATTTCC
AACGAGAAGAATCAATTCCC

Downstream 100 bases:

>100_bases
AAAATCAGATGTATTTTCGTTCAAATCGTTTGGTTGTAGTTTGTTGTCTGGGAGACGCTTGCGGGAACAAAAATGAGAGA
TTCTAGGGAATCAATGCTAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MNQSKEVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKIILESLKWLGVNWDEGPEVGG
EYGPYIQSQRLNIYKEYTEKLLKEKKAYRCFCTQEELEAKKKQSEAMGVPYVYDGLHANMSDEEVQEKLKQGIPYSVRFK
TPSKTLIINDIIQGKVKFETKLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALGYNIPEF
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSTDGREFLPGDELEKIFDVHRCSKSPSTFDVFKK
PKGSDEEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLNISKVLENLLPFLMDRKDIPAEVKNVNNPILTSIVESVRVY
LDNLTQAPDYVAEFFVTDLKIQSEETIGFLKDGEGPKVVNEFYEILKNSDPKTDEDYKNLMSKVGEVTGQKGKTLYMPIR
AATTGKSAGLELPILFPLLGKEKLLQRIEKTSKETGISLSN

Sequences:

>Translated_521_residues
MNQSKEVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKIILESLKWLGVNWDEGPEVGG
EYGPYIQSQRLNIYKEYTEKLLKEKKAYRCFCTQEELEAKKKQSEAMGVPYVYDGLHANMSDEEVQEKLKQGIPYSVRFK
TPSKTLIINDIIQGKVKFETKLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALGYNIPEF
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSTDGREFLPGDELEKIFDVHRCSKSPSTFDVFKK
PKGSDEEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLNISKVLENLLPFLMDRKDIPAEVKNVNNPILTSIVESVRVY
LDNLTQAPDYVAEFFVTDLKIQSEETIGFLKDGEGPKVVNEFYEILKNSDPKTDEDYKNLMSKVGEVTGQKGKTLYMPIR
AATTGKSAGLELPILFPLLGKEKLLQRIEKTSKETGISLSN
>Mature_521_residues
MNQSKEVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKIILESLKWLGVNWDEGPEVGG
EYGPYIQSQRLNIYKEYTEKLLKEKKAYRCFCTQEELEAKKKQSEAMGVPYVYDGLHANMSDEEVQEKLKQGIPYSVRFK
TPSKTLIINDIIQGKVKFETKLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALGYNIPEF
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSTDGREFLPGDELEKIFDVHRCSKSPSTFDVFKK
PKGSDEEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLNISKVLENLLPFLMDRKDIPAEVKNVNNPILTSIVESVRVY
LDNLTQAPDYVAEFFVTDLKIQSEETIGFLKDGEGPKVVNEFYEILKNSDPKTDEDYKNLMSKVGEVTGQKGKTLYMPIR
AATTGKSAGLELPILFPLLGKEKLLQRIEKTSKETGISLSN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=336, Percent_Identity=40.1785714285714, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI62241042, Length=259, Percent_Identity=25.4826254826255, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4826960, Length=304, Percent_Identity=24.6710526315789, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=514, Percent_Identity=34.2412451361868, Blast_Score=295, Evalue=6e-81,
Organism=Escherichia coli, GI226510925, Length=255, Percent_Identity=32.5490196078431, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786895, Length=219, Percent_Identity=23.2876712328767, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=306, Percent_Identity=41.5032679738562, Blast_Score=228, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6324540, Length=329, Percent_Identity=42.8571428571429, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321192, Length=240, Percent_Identity=28.75, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=116, Percent_Identity=32.7586206896552, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=317, Percent_Identity=43.5331230283912, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24649466, Length=257, Percent_Identity=25.6809338521401, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 58810; Mature: 58810

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSKEVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKI
CCCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
ILESLKWLGVNWDEGPEVGGEYGPYIQSQRLNIYKEYTEKLLKEKKAYRCFCTQEELEAK
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHH
KKQSEAMGVPYVYDGLHANMSDEEVQEKLKQGIPYSVRFKTPSKTLIINDIIQGKVKFET
HHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHCCCCEEEE
KLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALGYNIPEF
EEEEEEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCCCCH
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSTDGREFLPGDELE
HHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHH
KIFDVHRCSKSPSTFDVFKKPKGSDEEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLN
HHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
ISKVLENLLPFLMDRKDIPAEVKNVNNPILTSIVESVRVYLDNLTQAPDYVAEFFVTDLK
HHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHE
IQSEETIGFLKDGEGPKVVNEFYEILKNSDPKTDEDYKNLMSKVGEVTGQKGKTLYMPIR
ECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEE
AATTGKSAGLELPILFPLLGKEKLLQRIEKTSKETGISLSN
EECCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNQSKEVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKI
CCCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
ILESLKWLGVNWDEGPEVGGEYGPYIQSQRLNIYKEYTEKLLKEKKAYRCFCTQEELEAK
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHH
KKQSEAMGVPYVYDGLHANMSDEEVQEKLKQGIPYSVRFKTPSKTLIINDIIQGKVKFET
HHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHCCCCEEEE
KLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALGYNIPEF
EEEEEEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCCCCH
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSTDGREFLPGDELE
HHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHH
KIFDVHRCSKSPSTFDVFKKPKGSDEEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLN
HHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
ISKVLENLLPFLMDRKDIPAEVKNVNNPILTSIVESVRVYLDNLTQAPDYVAEFFVTDLK
HHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHE
IQSEETIGFLKDGEGPKVVNEFYEILKNSDPKTDEDYKNLMSKVGEVTGQKGKTLYMPIR
ECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEE
AATTGKSAGLELPILFPLLGKEKLLQRIEKTSKETGISLSN
EECCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA