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The map label for this gene is choD

Identifier: 45659024

GI number: 45659024

Start: 3928836

End: 3930560

Strand: Reverse

Name: choD

Synonym: LIC13202

Alternate gene names: NA

Gene position: 3930560-3928836 (Counterclockwise)

Preceding gene: 45659025

Following gene: 45659023

Centisome position: 91.9

GC content: 37.39

Gene sequence:

>1725_bases
ATGAAAAAATACGAAGCGGTTGTAATTGGTACTGGATTTGGCGGAGCCGTGAACGGTTGTAGATTGAGTAAAAAATGGCC
GGGTAAAGTTTTGATTTTAGAACGTGGAAAACGTTATCCCAAGGGCTCTTTCCCTCGTTCCCCTCACGATATGTCCAAGA
ATTTTTGGAATGTTCCAGAGGAGAACAGTTCCAAAGTTCGTCCTAAAAAACTTTCGAAATTAAATCAGACGGGTTTGTTT
GACATTCGAAATTACAAAAATATGGATGTGGTTATTTCTGCCGGACTTGGCGGAGGGTCCTTGATTTATGCGAATGTATT
TTTAGAACCTCCGGATCATATCTTTGATGAGCGTTGGCCTGCGAACATAAAAAAGAAAAGTTTAACACCGTATTATAAAA
TTGTAAAAGACGTTTTGGGCTCCAGGCCAATTCCTTTAAACGATGATCCTCGTAGAAAAATTGTTCGAACAACTCTTTAC
CAAAAGTTTGCAAAACACACTGGCAGAGAATCTAAATTGGCGGACATAAACGTATTTTTCGGAAACGATTTTAAAAAACC
AACTCCAATAGGACTTCAAGAAAAAAATAGATACGGAGCTGTTCAGACTTCTTGTACGTATTGCGCGGAATGTGATATAG
GATGTAATACTCATTCTAAAAATACTTTAGATCTAAATTATCTTTTTGTTGCGGAGAATAAGTATAAGGCGGAAGTCAGA
ACGGAACAGCTGGTTCAAAAAATTGTTCCTCTTGGGCCGGATGGAAATGATAATTCCTCTTATCACGGCGAAAACGGGTA
TCGTGTTTATTTTAGAAATTTTTCCGATCAAAAAAGGGAGCTTATCTCCGTCATTACAAAACGTGTAATAGTTTCCGCAG
GAACATTAGGAAGTACTGAACTTCTTTTAAAATGTAAGGAAGAATTTAAAACACTTCCAGACATTTCGGATCATCTAGGA
AAACAATTTTCAGGCAACGGAGATTTTTTATCTTTTGCGATCAAAGGAAAAGAACCTGCTAATCCGAATTACGGTCCGGT
GATCACTCAATATACCGATTATAATTTATTTAAGAGTTATAATCCTAAAAAGGCTTTTATACTTCAAGATGCTAGTTATC
CAGTCTTTGCTTCTTATTTTGCTGCGGCTTCTGTTCCATGGTTTTTAAGAATTGGATTTTTCTTTCATAAGTTCGTGGAA
CTTTTTAAAAAATTTCTAAATGGAAAAATTTTCGGAAAGATAGGTTATCTGATGGGCGAACTGTTGAAAGGTGACCTTTC
TTATACGTCTGCGGTGTTGTTGTGTATGGGAATCGATCGAGCGGATGGTACAATGGTTTTAGACCGTAAAAAGAATCTTC
AGATTCAATGGCCTCAGAAAAATAATATGTCTCTTTATGAAGCCATTTTAAGTGTGATGAAGGACTTTGCTTCGTTTATC
AAAACTGATTTTTATTTTCCACTTCCTACTTGGTCATGGCCGATTCGAAATAACGTTTCTGTGCATCCACTTGGTGGTTG
TATTCTTGGAAATTCTAAAACCAATTCGGTAACGTCTGCAGATTTTAAAACGTTTGGACAGGTTTTTTCGTATCAAGGCC
TTTACGTTGCAGATGGAAGTCTTTCTCCTACTGCGATTGGGGCAAATCCTTCTATGACAATTGCTGCTCTTTCTGAAAAA
GTCGCTGAGGGAATTACAGGAATCAAACCGACTGCCAATCTTTAG

Upstream 100 bases:

>100_bases
TTTGTTTCTTTTTTAGGAGCTTTGGTCATTGGCCTATACCTTCTGAATTATTTTGAATTTAGTTTATTTTAATATATAAA
TATTCTTATTCGGGGTTGTC

Downstream 100 bases:

>100_bases
CTAAGATGGATATAACTTTATAATATAGAGAATGTGGGAACTCATACTTTTTATAGGTTCTTGGATAAATCAAAATAAGG
AGACTTAATTTGTGGGAACT

Product: cholesterol oxidase precursor

Products: cholest-4-en-3-one; H2O2

Alternate protein names: Glucose-Methanol-Choline Oxidoreductase; FAD Dependent Oxidoreductase; GMC Oxidoreductase; Oxidoreductase; Choline Dehydrogenase-Like Flavoprotein; Choline Dehydrogenase; Cholesterol Oxidase ChoD; Oxidase; Oxidoreductase Glucose-Methanol-Choline Family; FAD-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase; Gmc Oxidoreductase Family Protein; Ferredoxin Domain Gmc Oxidoreductase; Choline Dehydrogenase And Related Flavoprotein-Like Protein; Cholesterol Oxidase ChoD; GMC Family Oxidoreductase; Cholesterol-O2 Oxidoreductase; Fad-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase; GMC Oxidoreductase Family Protein; Cholesterol Oxidase Chod; GMC Oxidoreductase Family; Cholesterol Oxidase Chob

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKKYEAVVIGTGFGGAVNGCRLSKKWPGKVLILERGKRYPKGSFPRSPHDMSKNFWNVPEENSSKVRPKKLSKLNQTGLF
DIRNYKNMDVVISAGLGGGSLIYANVFLEPPDHIFDERWPANIKKKSLTPYYKIVKDVLGSRPIPLNDDPRRKIVRTTLY
QKFAKHTGRESKLADINVFFGNDFKKPTPIGLQEKNRYGAVQTSCTYCAECDIGCNTHSKNTLDLNYLFVAENKYKAEVR
TEQLVQKIVPLGPDGNDNSSYHGENGYRVYFRNFSDQKRELISVITKRVIVSAGTLGSTELLLKCKEEFKTLPDISDHLG
KQFSGNGDFLSFAIKGKEPANPNYGPVITQYTDYNLFKSYNPKKAFILQDASYPVFASYFAAASVPWFLRIGFFFHKFVE
LFKKFLNGKIFGKIGYLMGELLKGDLSYTSAVLLCMGIDRADGTMVLDRKKNLQIQWPQKNNMSLYEAILSVMKDFASFI
KTDFYFPLPTWSWPIRNNVSVHPLGGCILGNSKTNSVTSADFKTFGQVFSYQGLYVADGSLSPTAIGANPSMTIAALSEK
VAEGITGIKPTANL

Sequences:

>Translated_574_residues
MKKYEAVVIGTGFGGAVNGCRLSKKWPGKVLILERGKRYPKGSFPRSPHDMSKNFWNVPEENSSKVRPKKLSKLNQTGLF
DIRNYKNMDVVISAGLGGGSLIYANVFLEPPDHIFDERWPANIKKKSLTPYYKIVKDVLGSRPIPLNDDPRRKIVRTTLY
QKFAKHTGRESKLADINVFFGNDFKKPTPIGLQEKNRYGAVQTSCTYCAECDIGCNTHSKNTLDLNYLFVAENKYKAEVR
TEQLVQKIVPLGPDGNDNSSYHGENGYRVYFRNFSDQKRELISVITKRVIVSAGTLGSTELLLKCKEEFKTLPDISDHLG
KQFSGNGDFLSFAIKGKEPANPNYGPVITQYTDYNLFKSYNPKKAFILQDASYPVFASYFAAASVPWFLRIGFFFHKFVE
LFKKFLNGKIFGKIGYLMGELLKGDLSYTSAVLLCMGIDRADGTMVLDRKKNLQIQWPQKNNMSLYEAILSVMKDFASFI
KTDFYFPLPTWSWPIRNNVSVHPLGGCILGNSKTNSVTSADFKTFGQVFSYQGLYVADGSLSPTAIGANPSMTIAALSEK
VAEGITGIKPTANL
>Mature_574_residues
MKKYEAVVIGTGFGGAVNGCRLSKKWPGKVLILERGKRYPKGSFPRSPHDMSKNFWNVPEENSSKVRPKKLSKLNQTGLF
DIRNYKNMDVVISAGLGGGSLIYANVFLEPPDHIFDERWPANIKKKSLTPYYKIVKDVLGSRPIPLNDDPRRKIVRTTLY
QKFAKHTGRESKLADINVFFGNDFKKPTPIGLQEKNRYGAVQTSCTYCAECDIGCNTHSKNTLDLNYLFVAENKYKAEVR
TEQLVQKIVPLGPDGNDNSSYHGENGYRVYFRNFSDQKRELISVITKRVIVSAGTLGSTELLLKCKEEFKTLPDISDHLG
KQFSGNGDFLSFAIKGKEPANPNYGPVITQYTDYNLFKSYNPKKAFILQDASYPVFASYFAAASVPWFLRIGFFFHKFVE
LFKKFLNGKIFGKIGYLMGELLKGDLSYTSAVLLCMGIDRADGTMVLDRKKNLQIQWPQKNNMSLYEAILSVMKDFASFI
KTDFYFPLPTWSWPIRNNVSVHPLGGCILGNSKTNSVTSADFKTFGQVFSYQGLYVADGSLSPTAIGANPSMTIAALSEK
VAEGITGIKPTANL

Specific function: Unknown

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.3.6

Molecular weight: Translated: 64302; Mature: 64302

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYEAVVIGTGFGGAVNGCRLSKKWPGKVLILERGKRYPKGSFPRSPHDMSKNFWNVPE
CCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCC
ENSSKVRPKKLSKLNQTGLFDIRNYKNMDVVISAGLGGGSLIYANVFLEPPDHIFDERWP
CCCCCCCHHHHHHCCCCCCEEECCCCCCEEEEEECCCCCEEEEEEEEECCCHHHHCCCCC
ANIKKKSLTPYYKIVKDVLGSRPIPLNDDPRRKIVRTTLYQKFAKHTGRESKLADINVFF
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
GNDFKKPTPIGLQEKNRYGAVQTSCTYCAECDIGCNTHSKNTLDLNYLFVAENKYKAEVR
CCCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHH
TEQLVQKIVPLGPDGNDNSSYHGENGYRVYFRNFSDQKRELISVITKRVIVSAGTLGSTE
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHH
LLLKCKEEFKTLPDISDHLGKQFSGNGDFLSFAIKGKEPANPNYGPVITQYTDYNLFKSY
HHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCHHHHCC
NPKKAFILQDASYPVFASYFAAASVPWFLRIGFFFHKFVELFKKFLNGKIFGKIGYLMGE
CCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHH
LLKGDLSYTSAVLLCMGIDRADGTMVLDRKKNLQIQWPQKNNMSLYEAILSVMKDFASFI
HHHCCHHHHHHHHHHEECCCCCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
KTDFYFPLPTWSWPIRNNVSVHPLGGCILGNSKTNSVTSADFKTFGQVFSYQGLYVADGS
HCCCEECCCCCCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCC
LSPTAIGANPSMTIAALSEKVAEGITGIKPTANL
CCCCEECCCCCEEHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKYEAVVIGTGFGGAVNGCRLSKKWPGKVLILERGKRYPKGSFPRSPHDMSKNFWNVPE
CCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCC
ENSSKVRPKKLSKLNQTGLFDIRNYKNMDVVISAGLGGGSLIYANVFLEPPDHIFDERWP
CCCCCCCHHHHHHCCCCCCEEECCCCCCEEEEEECCCCCEEEEEEEEECCCHHHHCCCCC
ANIKKKSLTPYYKIVKDVLGSRPIPLNDDPRRKIVRTTLYQKFAKHTGRESKLADINVFF
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
GNDFKKPTPIGLQEKNRYGAVQTSCTYCAECDIGCNTHSKNTLDLNYLFVAENKYKAEVR
CCCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHH
TEQLVQKIVPLGPDGNDNSSYHGENGYRVYFRNFSDQKRELISVITKRVIVSAGTLGSTE
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHH
LLLKCKEEFKTLPDISDHLGKQFSGNGDFLSFAIKGKEPANPNYGPVITQYTDYNLFKSY
HHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCHHHHCC
NPKKAFILQDASYPVFASYFAAASVPWFLRIGFFFHKFVELFKKFLNGKIFGKIGYLMGE
CCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHH
LLKGDLSYTSAVLLCMGIDRADGTMVLDRKKNLQIQWPQKNNMSLYEAILSVMKDFASFI
HHHCCHHHHHHHHHHEECCCCCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
KTDFYFPLPTWSWPIRNNVSVHPLGGCILGNSKTNSVTSADFKTFGQVFSYQGLYVADGS
HCCCEECCCCCCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCC
LSPTAIGANPSMTIAALSEKVAEGITGIKPTANL
CCCCEECCCCCEEHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cholesterol; O2

Specific reaction: cholesterol + O2 = cholest-4-en-3-one + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA