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The map label for this gene is lepA

Identifier: 45657863

GI number: 45657863

Start: 2419675

End: 2421477

Strand: Reverse

Name: lepA

Synonym: LIC12010

Alternate gene names: 45657863

Gene position: 2421477-2419675 (Counterclockwise)

Preceding gene: 45657864

Following gene: 45657862

Centisome position: 56.61

GC content: 38.77

Gene sequence:

>1803_bases
ATGAGTGATAAACAACAATTCATCCGAAACTTTTCAATCATAGCCCATATCGACCATGGAAAGTCTACTTTGGCAGACAG
ACTTTTAGAAATCGGTCAAGTAACAAACGATCGTACTAAAAAAGACCAGATTCTGGATTCGATGGACATAGAGAGAGAAA
GGGGAATCACAATCAAAGCCAATAATGCCACTTTTGATTATCTTGCGGAAGATGGAAATACATATATCATGAATCTTTTG
GATACTCCTGGTCACGTGGATTTTACCTACGAGGTTTCTCGTTCCTTAAAGGCATGTGAAGGAGTACTTTTAATTGTAGA
TGCAAGTCAGGGAGTAGAAGCACAAACTTTGGCAAACCTCTATCTCGCAATGGAGCAAGATTTGGAGATCCTTCCAGTAA
TGAACAAGATTGATCTTCCAGCTGCAGATGTGGAAAAAACGAAAATCCAAATCGAAGAAAGTTTAGGTTTGGATCCACAG
AAGGCGGTTGCGATTTCTGCAAAAACAGGACTCAATGTAAAAGAAGTTTTAGAACAAATCACCAAACAAATTCCATCACC
CAAGGGAAATTCGAATGCCCCTTTGAAGGCGCTCGTATATGATTCTTATTTTGATCCTTACATGGGAGTTGTGATTAAGA
TTCGAATTTTTGACGGTAGAATTAAAAAGGGTGATCGGATCTTGATGATGAGTACCGGAAAAGATTTTACGGTCAACGAA
GTAGGGATCAATCGAATTAATCTAACTCCTAAAGAAAGTTTAGAAACTGGGGAAGTAGGTTATATCATCGCCGGAATTAA
GAAGGTCTCGGACGCAAAAACTGGAGACACAGTTACGTTATTCTCTAATCCTACAAAAGAATCTGTGCCTGGTTATAAAG
AAGCCAAACCAATGGTATTTGCGGGTTTATTTCCGATTAATGGAGAACAATTTGACGAACTTGTGGACGCGATCGAAAAA
CTGAAACTTAACGATGCTGCTCTTGTTTTTGAAAAGGAAAGCTCTATTGCGTTAGGATTTGGATTTAGAGTTGGTTATTT
AGGGCTTCTGCATATGGAGATCGTTCAGGAAAGATTGGAAAGAGAATTCAATCTGGATCTGATCACCACTGCGCCTTCCG
TAAAATATATTATTCGAAGTAAGAATGGAGAAGTGGAAGAAATTGATAATCCTTCCCGTTTTCCGGAACCGATAACGATC
GAGTCTACGGAAGAACCTTATGTCAAAGCTACCGTAATCACACCGAACGAATACGTAGGGAATATCATGTCTCTTGCGAT
GGATAAAAGGGGAATTCAGTTAGATACTGTCTATCTTACACAAGATAAGGTGCAGTTAACGTATGAAATCCCTTTGGCCG
AACTTATATTCGAATTTTATGATAAACTCAAATCTTTTACGAGAGGTTATGCTTCTCTGGATTACGAACCTTCTGGATAT
AAGGCTTCTCAGCTCGTAAAAATGGATATTCTTGTAAATGGAGAGCCAGTAGACGCTCTTTCTATGATTGTACATAGGTC
TAAGGCGGAACAAAGAGGAAGAGAAATTATAGAGAAGTTAAAGGATCTGATTCCAAGGCATCAATTTATGATTCCTCTAC
AAGCCGCTGTAGGTGGAAAAATTCTGGCAAGAGAAAGTATTTCCGCTCTTCGTAAAAACGTAACTGCGAAGTGTTACGGA
GGGGATATTACTCGTAAGAAAAAACTATTAGAAAAACAAAAAGAAGGAAAAAAACGGATGAAACAGATCGGAAACGTAGA
AATTCCTCAGGAAGCGTTTTTGGCGGTTCTGAAAACAAGTTAA

Upstream 100 bases:

>100_bases
CTGTAAAATTCTAAATTGTAATAGTTCCCACAAATTTTATTACAAAAGTATTTTGCTTTTCAGACTAGGGTGGGACCAAA
AATCATCAGATTGAACCCTC

Downstream 100 bases:

>100_bases
TGTTTTTCTAATAAACTTTTGATTGCAACTTTTCATAAAATAAAATCTAGGAAGAACGGAATATAAGTTCTATTGCGTGG
TGAACCGTTTATGTTTGAGT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MSDKQQFIRNFSIIAHIDHGKSTLADRLLEIGQVTNDRTKKDQILDSMDIERERGITIKANNATFDYLAEDGNTYIMNLL
DTPGHVDFTYEVSRSLKACEGVLLIVDASQGVEAQTLANLYLAMEQDLEILPVMNKIDLPAADVEKTKIQIEESLGLDPQ
KAVAISAKTGLNVKEVLEQITKQIPSPKGNSNAPLKALVYDSYFDPYMGVVIKIRIFDGRIKKGDRILMMSTGKDFTVNE
VGINRINLTPKESLETGEVGYIIAGIKKVSDAKTGDTVTLFSNPTKESVPGYKEAKPMVFAGLFPINGEQFDELVDAIEK
LKLNDAALVFEKESSIALGFGFRVGYLGLLHMEIVQERLEREFNLDLITTAPSVKYIIRSKNGEVEEIDNPSRFPEPITI
ESTEEPYVKATVITPNEYVGNIMSLAMDKRGIQLDTVYLTQDKVQLTYEIPLAELIFEFYDKLKSFTRGYASLDYEPSGY
KASQLVKMDILVNGEPVDALSMIVHRSKAEQRGREIIEKLKDLIPRHQFMIPLQAAVGGKILARESISALRKNVTAKCYG
GDITRKKKLLEKQKEGKKRMKQIGNVEIPQEAFLAVLKTS

Sequences:

>Translated_600_residues
MSDKQQFIRNFSIIAHIDHGKSTLADRLLEIGQVTNDRTKKDQILDSMDIERERGITIKANNATFDYLAEDGNTYIMNLL
DTPGHVDFTYEVSRSLKACEGVLLIVDASQGVEAQTLANLYLAMEQDLEILPVMNKIDLPAADVEKTKIQIEESLGLDPQ
KAVAISAKTGLNVKEVLEQITKQIPSPKGNSNAPLKALVYDSYFDPYMGVVIKIRIFDGRIKKGDRILMMSTGKDFTVNE
VGINRINLTPKESLETGEVGYIIAGIKKVSDAKTGDTVTLFSNPTKESVPGYKEAKPMVFAGLFPINGEQFDELVDAIEK
LKLNDAALVFEKESSIALGFGFRVGYLGLLHMEIVQERLEREFNLDLITTAPSVKYIIRSKNGEVEEIDNPSRFPEPITI
ESTEEPYVKATVITPNEYVGNIMSLAMDKRGIQLDTVYLTQDKVQLTYEIPLAELIFEFYDKLKSFTRGYASLDYEPSGY
KASQLVKMDILVNGEPVDALSMIVHRSKAEQRGREIIEKLKDLIPRHQFMIPLQAAVGGKILARESISALRKNVTAKCYG
GDITRKKKLLEKQKEGKKRMKQIGNVEIPQEAFLAVLKTS
>Mature_599_residues
SDKQQFIRNFSIIAHIDHGKSTLADRLLEIGQVTNDRTKKDQILDSMDIERERGITIKANNATFDYLAEDGNTYIMNLLD
TPGHVDFTYEVSRSLKACEGVLLIVDASQGVEAQTLANLYLAMEQDLEILPVMNKIDLPAADVEKTKIQIEESLGLDPQK
AVAISAKTGLNVKEVLEQITKQIPSPKGNSNAPLKALVYDSYFDPYMGVVIKIRIFDGRIKKGDRILMMSTGKDFTVNEV
GINRINLTPKESLETGEVGYIIAGIKKVSDAKTGDTVTLFSNPTKESVPGYKEAKPMVFAGLFPINGEQFDELVDAIEKL
KLNDAALVFEKESSIALGFGFRVGYLGLLHMEIVQERLEREFNLDLITTAPSVKYIIRSKNGEVEEIDNPSRFPEPITIE
STEEPYVKATVITPNEYVGNIMSLAMDKRGIQLDTVYLTQDKVQLTYEIPLAELIFEFYDKLKSFTRGYASLDYEPSGYK
ASQLVKMDILVNGEPVDALSMIVHRSKAEQRGREIIEKLKDLIPRHQFMIPLQAAVGGKILARESISALRKNVTAKCYGG
DITRKKKLLEKQKEGKKRMKQIGNVEIPQEAFLAVLKTS

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=51.414309484193, Blast_Score=641, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=268, Percent_Identity=32.089552238806, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI4503483, Length=148, Percent_Identity=39.1891891891892, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306283, Length=155, Percent_Identity=40.6451612903226, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI19923640, Length=155, Percent_Identity=40.6451612903226, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI25306287, Length=155, Percent_Identity=40.6451612903226, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI217272894, Length=141, Percent_Identity=36.1702127659575, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI217272892, Length=141, Percent_Identity=36.1702127659575, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI18390331, Length=154, Percent_Identity=37.012987012987, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=40.3669724770642, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=40.3669724770642, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=40.3669724770642, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966752, Length=233, Percent_Identity=27.8969957081545, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI34147630, Length=289, Percent_Identity=26.2975778546713, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI53729339, Length=221, Percent_Identity=28.5067873303167, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI53729337, Length=221, Percent_Identity=28.5067873303167, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI223555963, Length=282, Percent_Identity=26.241134751773, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI5729864, Length=282, Percent_Identity=26.241134751773, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=57.4074074074074, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=499, Percent_Identity=28.0561122244489, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1789738, Length=160, Percent_Identity=35.625, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790835, Length=137, Percent_Identity=35.7664233576642, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789559, Length=296, Percent_Identity=27.3648648648649, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=41.5032679738562, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17556745, Length=462, Percent_Identity=24.8917748917749, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=34.5679012345679, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=142, Percent_Identity=38.7323943661972, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=36.5671641791045, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=36.5671641791045, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=152, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115532065, Length=278, Percent_Identity=24.8201438848921, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=251, Percent_Identity=29.8804780876494, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=228, Percent_Identity=26.7543859649123, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115532067, Length=278, Percent_Identity=24.8201438848921, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=45.3177257525084, Blast_Score=553, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=37.3333333333333, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=37.3333333333333, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323098, Length=190, Percent_Identity=33.6842105263158, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=46.4285714285714, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=189, Percent_Identity=31.7460317460317, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=289, Percent_Identity=24.9134948096886, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=44.2404006677796, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI28574573, Length=229, Percent_Identity=36.2445414847162, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI221458488, Length=159, Percent_Identity=41.5094339622642, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585711, Length=152, Percent_Identity=35.5263157894737, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585713, Length=152, Percent_Identity=35.5263157894737, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=35.0649350649351, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24582462, Length=137, Percent_Identity=42.3357664233577, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357743, Length=167, Percent_Identity=32.9341317365269, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572034, Length=224, Percent_Identity=28.125, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281363316, Length=289, Percent_Identity=28.3737024221453, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864358, Length=289, Percent_Identity=28.3737024221453, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921738, Length=279, Percent_Identity=26.5232974910394, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67058; Mature: 66927

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKQQFIRNFSIIAHIDHGKSTLADRLLEIGQVTNDRTKKDQILDSMDIERERGITIKA
CCCHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCEEEEE
NNATFDYLAEDGNTYIMNLLDTPGHVDFTYEVSRSLKACEGVLLIVDASQGVEAQTLANL
CCCEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
YLAMEQDLEILPVMNKIDLPAADVEKTKIQIEESLGLDPQKAVAISAKTGLNVKEVLEQI
HHHHHCCCCEEECCCCCCCCCCCCCHHEEEEHHHCCCCCCCEEEEECCCCCCHHHHHHHH
TKQIPSPKGNSNAPLKALVYDSYFDPYMGVVIKIRIFDGRIKKGDRILMMSTGKDFTVNE
HHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEEEC
VGINRINLTPKESLETGEVGYIIAGIKKVSDAKTGDTVTLFSNPTKESVPGYKEAKPMVF
CCCEEECCCCHHCCCCCCCEEEEECHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEE
AGLFPINGEQFDELVDAIEKLKLNDAALVFEKESSIALGFGFRVGYLGLLHMEIVQERLE
EEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHH
REFNLDLITTAPSVKYIIRSKNGEVEEIDNPSRFPEPITIESTEEPYVKATVITPNEYVG
HHCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCHHHHH
NIMSLAMDKRGIQLDTVYLTQDKVQLTYEIPLAELIFEFYDKLKSFTRGYASLDYEPSGY
HHHHHHHCCCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
KASQLVKMDILVNGEPVDALSMIVHRSKAEQRGREIIEKLKDLIPRHQFMIPLQAAVGGK
CHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCC
ILARESISALRKNVTAKCYGGDITRKKKLLEKQKEGKKRMKQIGNVEIPQEAFLAVLKTS
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SDKQQFIRNFSIIAHIDHGKSTLADRLLEIGQVTNDRTKKDQILDSMDIERERGITIKA
CCHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCEEEEE
NNATFDYLAEDGNTYIMNLLDTPGHVDFTYEVSRSLKACEGVLLIVDASQGVEAQTLANL
CCCEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
YLAMEQDLEILPVMNKIDLPAADVEKTKIQIEESLGLDPQKAVAISAKTGLNVKEVLEQI
HHHHHCCCCEEECCCCCCCCCCCCCHHEEEEHHHCCCCCCCEEEEECCCCCCHHHHHHHH
TKQIPSPKGNSNAPLKALVYDSYFDPYMGVVIKIRIFDGRIKKGDRILMMSTGKDFTVNE
HHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEEEC
VGINRINLTPKESLETGEVGYIIAGIKKVSDAKTGDTVTLFSNPTKESVPGYKEAKPMVF
CCCEEECCCCHHCCCCCCCEEEEECHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEE
AGLFPINGEQFDELVDAIEKLKLNDAALVFEKESSIALGFGFRVGYLGLLHMEIVQERLE
EEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHH
REFNLDLITTAPSVKYIIRSKNGEVEEIDNPSRFPEPITIESTEEPYVKATVITPNEYVG
HHCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCHHHHH
NIMSLAMDKRGIQLDTVYLTQDKVQLTYEIPLAELIFEFYDKLKSFTRGYASLDYEPSGY
HHHHHHHCCCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
KASQLVKMDILVNGEPVDALSMIVHRSKAEQRGREIIEKLKDLIPRHQFMIPLQAAVGGK
CHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCC
ILARESISALRKNVTAKCYGGDITRKKKLLEKQKEGKKRMKQIGNVEIPQEAFLAVLKTS
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA