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The map label for this gene is fliM

Identifier: 45657699

GI number: 45657699

Start: 2231806

End: 2232831

Strand: Reverse

Name: fliM

Synonym: LIC11836

Alternate gene names: 45657699

Gene position: 2232831-2231806 (Counterclockwise)

Preceding gene: 45657700

Following gene: 45657697

Centisome position: 52.2

GC content: 39.47

Gene sequence:

>1026_bases
ATGACAGAAATTTTATCCCAAGATGAAATTGACGCGTTACTTAGCGCCATCAGTTCCGGTGAAGTAAGCGAATCAGATTA
TGCTTCCGTTTCTGAACAAAAAAAAGTAAAGATCTACGATTTTAAACGTCCGGATAAATTTTCAAAAGACCAAATCCGTA
CTTTACAAATGATGCATGAAACCTTTGCGCGTCTTGCAACCACAGGGCTTTCTGCTCAGCTAAGAGCGCTTGTTTCGGTT
CACGTTGCTTCTGTGGATCAGTTGACTTACGAAGAGTTCATTCGTTCCATTCCAAATCCCACAACACTTGCAGTAATCAA
CATGGACCCTCTTAGAGGTTCTGCAATCTTAGAAATTGATCCATCAATTTCTTTTACGATCATCGATCGTCTGTTTGGTG
GTAAAGGAGAACAGGCAAAAATTTCCAGGGAACTTTCTGAAATAGAAATGAGCGTAATGGAAGGAATTATTGTAAGAATT
TTAGGAAACATGAGAGAATCGTGGTCCACAGTGATAGACTTAAGACCTAGGCTTGGAAACATTGAAACAAACCCTCAATT
TGCTCAAGTAGTTCCTCCAAACGACATGGTGGTTTTGATTACTCTGGAAACTAAAATCGGAGAAGTGGAAGGGATGACGA
ATCTTTGTATTCCTTATATCACGATCGAACCGATCATCAATAAACTATCAGCACAATATTGGTATTCTTCCATTCGTAAG
GGAGAATTGGATGAAAACCGCGCCGTGATTCAGGAAAGATTGGATCAAGTAGCCATTCCTTTGATTGCGGAAGTTGGGTC
TGTGGACGTTTCCATTAACGATTTTATGAATCTTTCTATTGGAGATGTAGTAAAACTCGAAAACACTTCTACAAGATCAG
AGATGATCGTAAAAGTAGGAGAAAGAAAAAAGTTCAAATGCCTTCCTGGAAGAGTAGGAAGCAGACTCGCCATTCAGATC
GGTGAAAGAGTAGAAGATATTCCAGATGAACTTTTGGGTTCTACTCGTTCTGAACAAGAATATTAG

Upstream 100 bases:

>100_bases
GTCTTCGTGCCAAATATGAATATACTTACACTCTCCGAAAAGTTCCTGATGAACAGGGAGGTTTTTGGAAAGTGTCTAAC
TTAGTAGCAAAGGTAAAGAA

Downstream 100 bases:

>100_bases
AATTCTTTGAAGTTATTGGTAGTATGTTTTAATTTCATTTTGAAATTTTAAAAACTCAATTTCTAAACTTCTAAAAATTT
TCAAAAAGATTTTTGAATGC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTEILSQDEIDALLSAISSGEVSESDYASVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHETFARLATTGLSAQLRALVSV
HVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRI
LGNMRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRK
GELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSIGDVVKLENTSTRSEMIVKVGERKKFKCLPGRVGSRLAIQI
GERVEDIPDELLGSTRSEQEY

Sequences:

>Translated_341_residues
MTEILSQDEIDALLSAISSGEVSESDYASVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHETFARLATTGLSAQLRALVSV
HVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRI
LGNMRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRK
GELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSIGDVVKLENTSTRSEMIVKVGERKKFKCLPGRVGSRLAIQI
GERVEDIPDELLGSTRSEQEY
>Mature_340_residues
TEILSQDEIDALLSAISSGEVSESDYASVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHETFARLATTGLSAQLRALVSVH
VASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRIL
GNMRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRKG
ELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSIGDVVKLENTSTRSEMIVKVGERKKFKCLPGRVGSRLAIQIG
ERVEDIPDELLGSTRSEQEY

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=339, Percent_Identity=28.9085545722714, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 38110; Mature: 37979

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEILSQDEIDALLSAISSGEVSESDYASVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHE
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
TFARLATTGLSAQLRALVSVHVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEID
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEC
PSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRILGNMRESWSTVIDLRPRLGN
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
IETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRK
CCCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHCC
GELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSIGDVVKLENTSTRSEMIVKVG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEEECCCCCHHHHHHHHC
ERKKFKCLPGRVGSRLAIQIGERVEDIPDELLGSTRSEQEY
CCCCEEECCCCCCCEEEEHHHHHHHHCCHHHHCCCCCCCCC
>Mature Secondary Structure 
TEILSQDEIDALLSAISSGEVSESDYASVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHE
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
TFARLATTGLSAQLRALVSVHVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEID
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEC
PSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRILGNMRESWSTVIDLRPRLGN
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
IETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRK
CCCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHCC
GELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSIGDVVKLENTSTRSEMIVKVG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEEECCCCCHHHHHHHHC
ERKKFKCLPGRVGSRLAIQIGERVEDIPDELLGSTRSEQEY
CCCCEEECCCCCCCEEEEHHHHHHHHCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]