Click here to switch to the map view.

The map label for this gene is uvrD

Identifier: 45657496

GI number: 45657496

Start: 1988168

End: 1990366

Strand: Direct

Name: uvrD

Synonym: LIC11624

Alternate gene names: 45657496

Gene position: 1988168-1990366 (Clockwise)

Preceding gene: 45657495

Following gene: 45657497

Centisome position: 46.48

GC content: 36.38

Gene sequence:

>2199_bases
GTGGACTCCTCATTTTTATCCGATTTAAACGAAGAACAAAAAAAAGCTGTCTTACAAGTAAACGGGCCGGTTTTAATTTT
GGCGGGAGCAGGTTCCGGTAAAACAAGAGTTATCACTCATAGAATTGCAAATCTTCTAATCAATCATGGGATCGATCGTA
TCTGTGCAGTAACCTTTACCAATAAGGCGGCTGCGGAGATGGTAGAAAGAGTAAAAAAAATAGTTCCTTTTTTACCCGCA
AACGTTCAGATCAAAACCTTCCATTCTCTTTGTTTGTATATTTTAAGAAGAGAAGCTTCCTTTTTCGGATTTGATAACGG
TTTTACAATTTATGATACCACTCTTCAAGAATCACTTTTAAAACAAGTAATTAAGGATCTTTCTTTGGATCCTAAATTTT
ATAAACCCTCCACACTTGGAAACTATATAAGCGGTTTGAAAGATAAAATGTTGTCTCCTGAAAGTTATTTGGAAAAAGAA
GGTCGCAACGATTTTTCTAAGGCCGTATCTGCAATTTATAAGGAATACGAAAAAAGAAAGGATGCAAATTATGCTTTCGA
TTTCGGAGATCTGATATGGAAAACGGTTCAACTATTTCAAAAATCTTCGGATGCAATTTCTAAATACCGTCATAAGTGGG
AATACGTGATGGTAGATGAATATCAAGATACGAATAAGGTTCAATACGAACTTGTACTTCTTCTTGCAGGAGAAAAAAGA
AACCTATGTGTGGTTGGAGACGATGATCAATCTATTTATTCTTGGAGAGGAGCTGACATAGGAAACATTCTGAATTTCGA
AAAAGATTTTCCAGAATCAGTGGTGATCAAACTGGAAGAAAACTATCGTTCTACATCTAATATTATATTAGCTGCTTCTA
ATGTAATTTCAAACAACACTCAGAGAAAAGAAAAGGAAATATTTACGAATAACCCGGAAGGTGCTCCGGTTGTGTTAAAC
GAATTTGAAAATGAATCTGAGGAGGCTCACGGAGTGATCACTAGAATACGCTCCGCGTATTCCGGAGGAACGGAATATAA
GAATATTGCAATATTCTACAGAACTAATTCTCAGTCCAGATATTTTGAAGAAGCTTTGAGAAATGTAGGAATTCCGTATA
AAATTTTCGGAGGTTTCCGTTTTTTTGACAGAGCGGAAATTAAGGATTTGATTGCATATTTAAACGTGGTTTCGAACCCT
CTGGATAGCGTTTCGCTTCTTAGAATCATAAATTATCCTCCGAGAGGAATTGGGGATTCTGGAGTTGAAAAAATTCGAGA
GTTTTCACTCGAAAAAGGAATTTCCATTTTGGAAGTTTTGGGTCAAGAAGACATTCCTCTCAAAAAAGCAGCTAAGTCTA
AAGGAAAGGAACTTTATAATTTATTTTGCGATTTGATCGAAAAATCGGAAAAAGGTTTGTCTCCTTCCGAAATCGCATTA
GAACTTTTGAATCGTTCCGGTCTCATGTCTCATCTCAAAGATGAAGGAACGGAAGAATCTGTAGCTCGTTTAGAAAACCT
TCAAGAATTAGTCAATTCGATAGAGGAATACGAAAAAAATTCGGATTCTCCTTCTTTGGAAGAATATTTAAATCAGATTA
GTTTGATTACAAGCGAAGAAGATTCTAAAGAACTCACCGATTATGTGAATCTAATGACTGTGCATAACGCAAAAGGACTT
GAATTTGAAGTCGTTTTTCTTTCTGGATTGGAAGAGGGAACTTTTCCACATAGTATGAGTTTAGAAGAATCTCATTTTGG
GGACGAAGAAGAAAGAAGGCTTTTTTACGTTGCTCTTACTCGGGCTCGTGAGGAATTATTTCTAAGTTATTGTAGGACTT
CCAGAAAGTTTGGTAAGGTGGAAGATAGAATTCCTTCTCGATTTTTATCTGAAATTCCAAGAAAATGTTTTGGAAATAGA
GGTTATGTAAGTAGAGAACGAACTGCTAGAAAACCTCAGGGCCCTCCAGTGGCTTCTAAAATTAGACAAGCGAATAACGA
ATTGGAATCACACCACGCACCACCACCTGATCCAAATAATCTCTATCTAAAACCGGGAGATCGGGTAAAACACAAACAAT
TCGGGATAGGAACAATTTTGACTATTTCCGGTAGGGAAAAAAATACAAAGGTCGCCATCCGTTTTGGAAACGTAGAAAAG
AACTTTTTTGTTGCCTATACCCCGCTTGAGAAATTATAA

Upstream 100 bases:

>100_bases
ATTAAAGTATACTGGGGTTGCGGATCACCCTTTCACTAAATTTGAATCTGATAATGCATTTCAGTTTGTGTTTGGAATTG
GGGACTATAGGTTTTAGTCT

Downstream 100 bases:

>100_bases
GAAAGTGGAAAGTTTGTCGTTTTAAAATCCGATAATAATTAACGAAAAACAGGAAACTCAAGATGAAGAAAATACTCGCA
ATTTGTCTTTTATTTTTCTT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MDSSFLSDLNEEQKKAVLQVNGPVLILAGAGSGKTRVITHRIANLLINHGIDRICAVTFTNKAAAEMVERVKKIVPFLPA
NVQIKTFHSLCLYILRREASFFGFDNGFTIYDTTLQESLLKQVIKDLSLDPKFYKPSTLGNYISGLKDKMLSPESYLEKE
GRNDFSKAVSAIYKEYEKRKDANYAFDFGDLIWKTVQLFQKSSDAISKYRHKWEYVMVDEYQDTNKVQYELVLLLAGEKR
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESVVIKLEENYRSTSNIILAASNVISNNTQRKEKEIFTNNPEGAPVVLN
EFENESEEAHGVITRIRSAYSGGTEYKNIAIFYRTNSQSRYFEEALRNVGIPYKIFGGFRFFDRAEIKDLIAYLNVVSNP
LDSVSLLRIINYPPRGIGDSGVEKIREFSLEKGISILEVLGQEDIPLKKAAKSKGKELYNLFCDLIEKSEKGLSPSEIAL
ELLNRSGLMSHLKDEGTEESVARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEEDSKELTDYVNLMTVHNAKGL
EFEVVFLSGLEEGTFPHSMSLEESHFGDEEERRLFYVALTRAREELFLSYCRTSRKFGKVEDRIPSRFLSEIPRKCFGNR
GYVSRERTARKPQGPPVASKIRQANNELESHHAPPPDPNNLYLKPGDRVKHKQFGIGTILTISGREKNTKVAIRFGNVEK
NFFVAYTPLEKL

Sequences:

>Translated_732_residues
MDSSFLSDLNEEQKKAVLQVNGPVLILAGAGSGKTRVITHRIANLLINHGIDRICAVTFTNKAAAEMVERVKKIVPFLPA
NVQIKTFHSLCLYILRREASFFGFDNGFTIYDTTLQESLLKQVIKDLSLDPKFYKPSTLGNYISGLKDKMLSPESYLEKE
GRNDFSKAVSAIYKEYEKRKDANYAFDFGDLIWKTVQLFQKSSDAISKYRHKWEYVMVDEYQDTNKVQYELVLLLAGEKR
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESVVIKLEENYRSTSNIILAASNVISNNTQRKEKEIFTNNPEGAPVVLN
EFENESEEAHGVITRIRSAYSGGTEYKNIAIFYRTNSQSRYFEEALRNVGIPYKIFGGFRFFDRAEIKDLIAYLNVVSNP
LDSVSLLRIINYPPRGIGDSGVEKIREFSLEKGISILEVLGQEDIPLKKAAKSKGKELYNLFCDLIEKSEKGLSPSEIAL
ELLNRSGLMSHLKDEGTEESVARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEEDSKELTDYVNLMTVHNAKGL
EFEVVFLSGLEEGTFPHSMSLEESHFGDEEERRLFYVALTRAREELFLSYCRTSRKFGKVEDRIPSRFLSEIPRKCFGNR
GYVSRERTARKPQGPPVASKIRQANNELESHHAPPPDPNNLYLKPGDRVKHKQFGIGTILTISGREKNTKVAIRFGNVEK
NFFVAYTPLEKL
>Mature_732_residues
MDSSFLSDLNEEQKKAVLQVNGPVLILAGAGSGKTRVITHRIANLLINHGIDRICAVTFTNKAAAEMVERVKKIVPFLPA
NVQIKTFHSLCLYILRREASFFGFDNGFTIYDTTLQESLLKQVIKDLSLDPKFYKPSTLGNYISGLKDKMLSPESYLEKE
GRNDFSKAVSAIYKEYEKRKDANYAFDFGDLIWKTVQLFQKSSDAISKYRHKWEYVMVDEYQDTNKVQYELVLLLAGEKR
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESVVIKLEENYRSTSNIILAASNVISNNTQRKEKEIFTNNPEGAPVVLN
EFENESEEAHGVITRIRSAYSGGTEYKNIAIFYRTNSQSRYFEEALRNVGIPYKIFGGFRFFDRAEIKDLIAYLNVVSNP
LDSVSLLRIINYPPRGIGDSGVEKIREFSLEKGISILEVLGQEDIPLKKAAKSKGKELYNLFCDLIEKSEKGLSPSEIAL
ELLNRSGLMSHLKDEGTEESVARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEEDSKELTDYVNLMTVHNAKGL
EFEVVFLSGLEEGTFPHSMSLEESHFGDEEERRLFYVALTRAREELFLSYCRTSRKFGKVEDRIPSRFLSEIPRKCFGNR
GYVSRERTARKPQGPPVASKIRQANNELESHHAPPPDPNNLYLKPGDRVKHKQFGIGTILTISGREKNTKVAIRFGNVEK
NFFVAYTPLEKL

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=746, Percent_Identity=37.5335120643432, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI48994965, Length=664, Percent_Identity=39.1566265060241, Blast_Score=391, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=367, Percent_Identity=26.7029972752044, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=761, Percent_Identity=28.2522996057819, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324477, Length=650, Percent_Identity=22.9230769230769, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83291; Mature: 83291

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSFLSDLNEEQKKAVLQVNGPVLILAGAGSGKTRVITHRIANLLINHGIDRICAVTFT
CCCHHHHHCCHHHHHEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
NKAAAEMVERVKKIVPFLPANVQIKTFHSLCLYILRREASFFGFDNGFTIYDTTLQESLL
CHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHH
KQVIKDLSLDPKFYKPSTLGNYISGLKDKMLSPESYLEKEGRNDFSKAVSAIYKEYEKRK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHC
DANYAFDFGDLIWKTVQLFQKSSDAISKYRHKWEYVMVDEYQDTNKVQYELVLLLAGEKR
CCCCEECHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEECCCC
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESVVIKLEENYRSTSNIILAASNVISNNT
CEEEECCCCCCHHEECCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECHHHCCCC
QRKEKEIFTNNPEGAPVVLNEFENESEEAHGVITRIRSAYSGGTEYKNIAIFYRTNSQSR
HHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHH
YFEEALRNVGIPYKIFGGFRFFDRAEIKDLIAYLNVVSNPLDSVSLLRIINYPPRGIGDS
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHH
GVEKIREFSLEKGISILEVLGQEDIPLKKAAKSKGKELYNLFCDLIEKSEKGLSPSEIAL
HHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELLNRSGLMSHLKDEGTEESVARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
DSKELTDYVNLMTVHNAKGLEFEVVFLSGLEEGTFPHSMSLEESHFGDEEERRLFYVALT
HHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHHHEEEEHHHH
RAREELFLSYCRTSRKFGKVEDRIPSRFLSEIPRKCFGNRGYVSRERTARKPQGPPVASK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHH
IRQANNELESHHAPPPDPNNLYLKPGDRVKHKQFGIGTILTISGREKNTKVAIRFGNVEK
HHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCCEEEEEEECCCCCCEEEEEECCCCC
NFFVAYTPLEKL
CEEEEECCHHCC
>Mature Secondary Structure
MDSSFLSDLNEEQKKAVLQVNGPVLILAGAGSGKTRVITHRIANLLINHGIDRICAVTFT
CCCHHHHHCCHHHHHEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
NKAAAEMVERVKKIVPFLPANVQIKTFHSLCLYILRREASFFGFDNGFTIYDTTLQESLL
CHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHH
KQVIKDLSLDPKFYKPSTLGNYISGLKDKMLSPESYLEKEGRNDFSKAVSAIYKEYEKRK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHC
DANYAFDFGDLIWKTVQLFQKSSDAISKYRHKWEYVMVDEYQDTNKVQYELVLLLAGEKR
CCCCEECHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEECCCC
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESVVIKLEENYRSTSNIILAASNVISNNT
CEEEECCCCCCHHEECCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECHHHCCCC
QRKEKEIFTNNPEGAPVVLNEFENESEEAHGVITRIRSAYSGGTEYKNIAIFYRTNSQSR
HHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHH
YFEEALRNVGIPYKIFGGFRFFDRAEIKDLIAYLNVVSNPLDSVSLLRIINYPPRGIGDS
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHH
GVEKIREFSLEKGISILEVLGQEDIPLKKAAKSKGKELYNLFCDLIEKSEKGLSPSEIAL
HHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELLNRSGLMSHLKDEGTEESVARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
DSKELTDYVNLMTVHNAKGLEFEVVFLSGLEEGTFPHSMSLEESHFGDEEERRLFYVALT
HHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHHHEEEEHHHH
RAREELFLSYCRTSRKFGKVEDRIPSRFLSEIPRKCFGNRGYVSRERTARKPQGPPVASK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHH
IRQANNELESHHAPPPDPNNLYLKPGDRVKHKQFGIGTILTISGREKNTKVAIRFGNVEK
HHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCCEEEEEEECCCCCCEEEEEECCCCC
NFFVAYTPLEKL
CEEEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]