Click here to switch to the map view.

The map label for this gene is fliN

Identifier: 45657248

GI number: 45657248

Start: 1687104

End: 1688168

Strand: Direct

Name: fliN

Synonym: LIC11370

Alternate gene names: 45657248

Gene position: 1687104-1688168 (Clockwise)

Preceding gene: 45657247

Following gene: 45657249

Centisome position: 39.44

GC content: 43.38

Gene sequence:

>1065_bases
ATGGGTGAAGGTTCCCTATCACAGGAAGAAATAGACGCATTACTCGCCGGAGCAAGCGATTCGTTTGATCCGGGGACTGT
GGCACCTGCAGCCACACAAAAAGAAGTTCCAGGGATGTCTCCTGTGGATCGGGATATTTTATCGGATCTTTTAGCTTCTT
GTTTTCAAGTTGCAGGAAACACGTTAGGCGCTGTTCTTTCTCGTTCTTCTGCATTTTTAAATCCAAACGTAGAAACTAAA
ACTCGAAAGGATATAGAATCCGAATTAAAATCCGGATCTTTTCTTTTGTATTCTACACTTTCTGGAAGTGTAAATGGAAG
AGTAGTTTTAGCAATGTCTGCCGAAAATGCGGTAAAAATTTCCAATTCAATGATGGGCGGTTTCGATTCTGGAGAATTGG
ACGAAGCTCAGATGCAAACACTTCGAGATTCTCTGACTCCTGTAATGGGAGCTTTGATTTCCCAGATCAGTACAAAAACG
GGTGGAGGAGTCAATGGATCTCCTTCCGAAACTAGAAATGTAACTTCTCCTGCGGCTTTGGTTTTGCCAGATGGGGAAAC
TTTGATTCGAGTATTCTTTAATCTTACGATCGAAAATATACCTTCTTTTAGAGTTCAATTTTTACTCTCACTTTCTACTG
CTTCGGATCTTTTAAATCTTTATCGCCGTTCCGGAAGTGCAGGTGGTGATATGAGTGGAATGGGGATGGGAGGTATGGGC
GGAATGGGAATGGCCCCCGGTTCCATGTCTGTCAAATCGGTCGCATTCCCTAATTTAGGAACTGCGAGTGGTGCTTCTAA
TACTCCCAATTTGAATCTACTGATGGACGTTCAGATGAGCGTTACTGTGGAACTGGGGAGAACCAAGATGTATATCAAGG
ACATTTTAGGTTTAGGGGAAGGTTCTATCATAGAGTTGGATAAACTCGCAGGTGAGCCGGTAGATCTTTTAGTAAATGGC
AAGCTGATTGCAAAAGGTGAGGTCGTGGTCATCGACGAAAATTTTGGAGTGCGTGTAACGGATATAGTAAGTCCTATCGA
TCGGATTAAGCCGGAGGGGAAGTGA

Upstream 100 bases:

>100_bases
ACCTAAAAATTGGATGAAAACAGACTGGAAAGAAAAAAAGAGGTGACAAAAACTCTTTTCTTATGTCTCATTAACTTAAA
ACTCCCGGAGAATCCCCAAA

Downstream 100 bases:

>100_bases
GATTTTTGAAAACTTCCCAAAGTAATGGAAAGAAAACGATTTCCGTTCTTTCTCTTTTGATTATATTCAATTTTACTGTT
ATTTCTTTATCGGCCCAATC

Product: flagellar motor switch protein

Products: NA

Alternate protein names: CheY-P phosphatase FliY [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MGEGSLSQEEIDALLAGASDSFDPGTVAPAATQKEVPGMSPVDRDILSDLLASCFQVAGNTLGAVLSRSSAFLNPNVETK
TRKDIESELKSGSFLLYSTLSGSVNGRVVLAMSAENAVKISNSMMGGFDSGELDEAQMQTLRDSLTPVMGALISQISTKT
GGGVNGSPSETRNVTSPAALVLPDGETLIRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSAGGDMSGMGMGGMG
GMGMAPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGEGSIIELDKLAGEPVDLLVNG
KLIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK

Sequences:

>Translated_354_residues
MGEGSLSQEEIDALLAGASDSFDPGTVAPAATQKEVPGMSPVDRDILSDLLASCFQVAGNTLGAVLSRSSAFLNPNVETK
TRKDIESELKSGSFLLYSTLSGSVNGRVVLAMSAENAVKISNSMMGGFDSGELDEAQMQTLRDSLTPVMGALISQISTKT
GGGVNGSPSETRNVTSPAALVLPDGETLIRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSAGGDMSGMGMGGMG
GMGMAPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGEGSIIELDKLAGEPVDLLVNG
KLIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK
>Mature_353_residues
GEGSLSQEEIDALLAGASDSFDPGTVAPAATQKEVPGMSPVDRDILSDLLASCFQVAGNTLGAVLSRSSAFLNPNVETKT
RKDIESELKSGSFLLYSTLSGSVNGRVVLAMSAENAVKISNSMMGGFDSGELDEAQMQTLRDSLTPVMGALISQISTKTG
GGVNGSPSETRNVTSPAALVLPDGETLIRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSAGGDMSGMGMGGMGG
MGMAPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGEGSIIELDKLAGEPVDLLVNGK
LIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK

Specific function: Component of the flagellar switch. Binds CheY-P and increases its hydrolysis rate in vitro. May function constitutively to remove CheY-P around the flagellar switch to maintain an optimal level of CheY-P whereas CheC may function after addition of an attr

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=The switch is localized at the base of the flagellar motor, in or near the C-ring, attached to the MS-ring (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=103, Percent_Identity=46.6019417475728, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007597
- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF04509 CheC; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36835; Mature: 36703

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEGSLSQEEIDALLAGASDSFDPGTVAPAATQKEVPGMSPVDRDILSDLLASCFQVAGN
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCH
TLGAVLSRSSAFLNPNVETKTRKDIESELKSGSFLLYSTLSGSVNGRVVLAMSAENAVKI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEEE
SNSMMGGFDSGELDEAQMQTLRDSLTPVMGALISQISTKTGGGVNGSPSETRNVTSPAAL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
VLPDGETLIRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSAGGDMSGMGMGGMG
EECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GMGMAPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGE
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHCCCC
GSIIELDKLAGEPVDLLVNGKLIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK
CCEEEHHHHCCCCEEEEECCEEEECCEEEEEECCCCEEHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GEGSLSQEEIDALLAGASDSFDPGTVAPAATQKEVPGMSPVDRDILSDLLASCFQVAGN
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCH
TLGAVLSRSSAFLNPNVETKTRKDIESELKSGSFLLYSTLSGSVNGRVVLAMSAENAVKI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEEE
SNSMMGGFDSGELDEAQMQTLRDSLTPVMGALISQISTKTGGGVNGSPSETRNVTSPAAL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
VLPDGETLIRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSAGGDMSGMGMGGMG
EECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GMGMAPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGE
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHCCCC
GSIIELDKLAGEPVDLLVNGKLIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK
CCEEEHHHHCCCCEEEEECCEEEECCEEEEEECCCCEEHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1447979; 9384377; 1898932 [H]